Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells

Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells

The cytotoxicity of cell-free culture filtrates of 31 isolates of Vibrio cholerae O1 and O139, 5 reference strains and 26 clinical isolates, was tested on Madin Darby Bovine Kidney (MDBK) cells and Vero cells. The 3-[4,5- dimethylthiazol-2-y]-2, 5-diphenyltetrazolium bromide (MTT) test was used to...

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Main Authors: Haque, Quazi Manjurul, Mohammad , Najmiyatul Fadilah, Helaluddin , Abul Bashar Mohammed, Saeed , Mohammad
Format: Article
Language:English
Published: Faculty of Pharmacy, University of Karachi 2010
Subjects:
QR355 Virology
Online Access:http://irep.iium.edu.my/4307/
http://irep.iium.edu.my/4307/
http://irep.iium.edu.my/4307/1/Paper_PAkjps.pdf
id iium-4307
recordtype eprints
spelling iium-43072013-10-29T07:57:36Z http://irep.iium.edu.my/4307/ Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells Haque, Quazi Manjurul Mohammad , Najmiyatul Fadilah Helaluddin , Abul Bashar Mohammed Saeed , Mohammad QR355 Virology The cytotoxicity of cell-free culture filtrates of 31 isolates of Vibrio cholerae O1 and O139, 5 reference strains and 26 clinical isolates, was tested on Madin Darby Bovine Kidney (MDBK) cells and Vero cells. The 3-[4,5- dimethylthiazol-2-y]-2, 5-diphenyltetrazolium bromide (MTT) test was used to detect the effect of the filtrates on the proliferation and viability of cultured cell populations. The filtrates were prepared from serial ten-fold dilutions of inoculated AKI and APW broth media with and without the addition of polymyxin B. The APW culture filtrates of both V. cholerae O1 and O139 with and without added polymyxin B showed greater toxicity to MDBK cells as compared to AKI filtrates. The cytotoxicity of AKI-grown V. cholerae O139 to MDBK cells was greater than that of V. cholerae O1 grown in the same medium. The cytotoxicity of APW filtrates on Vero cells was low and only noted when polymyxin was added to the medium. Keywords: Vibrio cholerae, MDBK cells, Vero cells, Cytotoxicity and MTT test. Faculty of Pharmacy, University of Karachi 2010-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/4307/1/Paper_PAkjps.pdf Haque, Quazi Manjurul and Mohammad , Najmiyatul Fadilah and Helaluddin , Abul Bashar Mohammed and Saeed , Mohammad (2010) Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells. Pakistan Journal of Pharmaceutical Sciences, 23 (4). pp. 393-397. ISSN 11011-601X http://www.pakmedinet.com/PJPS
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QR355 Virology
spellingShingle QR355 Virology
Haque, Quazi Manjurul
Mohammad , Najmiyatul Fadilah
Helaluddin , Abul Bashar Mohammed
Saeed , Mohammad
Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells
description The cytotoxicity of cell-free culture filtrates of 31 isolates of Vibrio cholerae O1 and O139, 5 reference strains and 26 clinical isolates, was tested on Madin Darby Bovine Kidney (MDBK) cells and Vero cells. The 3-[4,5- dimethylthiazol-2-y]-2, 5-diphenyltetrazolium bromide (MTT) test was used to detect the effect of the filtrates on the proliferation and viability of cultured cell populations. The filtrates were prepared from serial ten-fold dilutions of inoculated AKI and APW broth media with and without the addition of polymyxin B. The APW culture filtrates of both V. cholerae O1 and O139 with and without added polymyxin B showed greater toxicity to MDBK cells as compared to AKI filtrates. The cytotoxicity of AKI-grown V. cholerae O139 to MDBK cells was greater than that of V. cholerae O1 grown in the same medium. The cytotoxicity of APW filtrates on Vero cells was low and only noted when polymyxin was added to the medium. Keywords: Vibrio cholerae, MDBK cells, Vero cells, Cytotoxicity and MTT test.
format Article
author Haque, Quazi Manjurul
Mohammad , Najmiyatul Fadilah
Helaluddin , Abul Bashar Mohammed
Saeed , Mohammad
author_facet Haque, Quazi Manjurul
Mohammad , Najmiyatul Fadilah
Helaluddin , Abul Bashar Mohammed
Saeed , Mohammad
author_sort Haque, Quazi Manjurul
title Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells
title_short Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells
title_full Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells
title_fullStr Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells
title_full_unstemmed Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells
title_sort cytotoxicity of vibrio cholerae of madin darby bovine kidney cells
publisher Faculty of Pharmacy, University of Karachi
publishDate 2010
url http://irep.iium.edu.my/4307/
http://irep.iium.edu.my/4307/
http://irep.iium.edu.my/4307/1/Paper_PAkjps.pdf
first_indexed 2023-09-18T20:12:29Z
last_indexed 2023-09-18T20:12:29Z
_version_ 1777407569881464832

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