Antioxidant properties of soft coral Dendronephthya sp

Present study was conducted to determine the antioxidant property of soft corals belong to the genus: Dendronephthya (Family: Nephtheidae) using α, α-Diphenyl-β-PicrylHydrazyl (DPPH) radical-scavenging and Ferric Thiocyanate (FTC) methods using vitamin E as a positive control. Crude extracts were pr...

Full description

Bibliographic Details
Main Authors:	Saad, Shahbudin, Darnis, Deny Susanti, Tengku Abdul Hamid , Tengku Haziyamin, John, Akbar, Bakhtiar, M. Taher
Format:	Article
Language:	English
Published:	A N S I Network 2011
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/4303/ http://irep.iium.edu.my/4303/ http://irep.iium.edu.my/4303/1/International_journal_of_pharmacology.pdf

id	iium-4303
recordtype	eprints
spelling	iium-43032014-12-09T00:26:03Z http://irep.iium.edu.my/4303/ Antioxidant properties of soft coral Dendronephthya sp Saad, Shahbudin Darnis, Deny Susanti Tengku Abdul Hamid , Tengku Haziyamin John, Akbar Bakhtiar, M. Taher RS Pharmacy and materia medica Present study was conducted to determine the antioxidant property of soft corals belong to the genus: Dendronephthya (Family: Nephtheidae) using α, α-Diphenyl-β-PicrylHydrazyl (DPPH) radical-scavenging and Ferric Thiocyanate (FTC) methods using vitamin E as a positive control. Crude extracts were prepared from 4 Dendronephthya sp. using aqueous, dichoromethane: methanol and methanol extraction. All crude extracts of Dendronephthya sp. exhibited antioxidant properties and the white spots appeared during the rapid screening using Dot-Blot and 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) staining where the concentration of crude extract was 1.00 g mL-1 against the DPPH concentration of 0.4 mM. In DPPH assay, not all crude extracts showed a significant value in antioxidant activities thus it could be considered as weak free radical species scavenger. The crude extract were diluted to 1000, 500, 250, 125, 63, 31, 15, 7, 4 and 2 μg mL-1, respectively and tested against highly diluted DPPH (0.06 mM). The IC50 for all crude extracts were greater than 1000 μg mL-1. The percentage of free radical scavenging exhibited by the crude extract was at 2 μg mL-1 concentration (the lowest concentration in serial dilution) with 0.81 to 2.89%. Ferric Thiocyanate (FTC) assay, showed absorbance ranges for control, vitamin E and sample were recorded as 0.012-0.858, 0.001-0.315 and 0.001-0.886, respectively. Inhibition percentage of all the crude extract was closer to the control indicated that they are weak lipid peroxidation inhibitor. However, the aqueous extract of species A and C showed higher inhibition percentage from other extract with the percentage value of 10.8 and 10.5% , respectively. A N S I Network 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/4303/1/International_journal_of_pharmacology.pdf Saad, Shahbudin and Darnis, Deny Susanti and Tengku Abdul Hamid , Tengku Haziyamin and John, Akbar and Bakhtiar, M. Taher (2011) Antioxidant properties of soft coral Dendronephthya sp. International Journal of Pharmacology, 7 (2). pp. 263-267. ISSN ISSN: 1811-7775 http://scialert.net/previous.php?issn=1811-7775
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Saad, Shahbudin Darnis, Deny Susanti Tengku Abdul Hamid , Tengku Haziyamin John, Akbar Bakhtiar, M. Taher Antioxidant properties of soft coral Dendronephthya sp
description	Present study was conducted to determine the antioxidant property of soft corals belong to the genus: Dendronephthya (Family: Nephtheidae) using α, α-Diphenyl-β-PicrylHydrazyl (DPPH) radical-scavenging and Ferric Thiocyanate (FTC) methods using vitamin E as a positive control. Crude extracts were prepared from 4 Dendronephthya sp. using aqueous, dichoromethane: methanol and methanol extraction. All crude extracts of Dendronephthya sp. exhibited antioxidant properties and the white spots appeared during the rapid screening using Dot-Blot and 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) staining where the concentration of crude extract was 1.00 g mL-1 against the DPPH concentration of 0.4 mM. In DPPH assay, not all crude extracts showed a significant value in antioxidant activities thus it could be considered as weak free radical species scavenger. The crude extract were diluted to 1000, 500, 250, 125, 63, 31, 15, 7, 4 and 2 μg mL-1, respectively and tested against highly diluted DPPH (0.06 mM). The IC50 for all crude extracts were greater than 1000 μg mL-1. The percentage of free radical scavenging exhibited by the crude extract was at 2 μg mL-1 concentration (the lowest concentration in serial dilution) with 0.81 to 2.89%. Ferric Thiocyanate (FTC) assay, showed absorbance ranges for control, vitamin E and sample were recorded as 0.012-0.858, 0.001-0.315 and 0.001-0.886, respectively. Inhibition percentage of all the crude extract was closer to the control indicated that they are weak lipid peroxidation inhibitor. However, the aqueous extract of species A and C showed higher inhibition percentage from other extract with the percentage value of 10.8 and 10.5% , respectively.
format	Article
author	Saad, Shahbudin Darnis, Deny Susanti Tengku Abdul Hamid , Tengku Haziyamin John, Akbar Bakhtiar, M. Taher
author_facet	Saad, Shahbudin Darnis, Deny Susanti Tengku Abdul Hamid , Tengku Haziyamin John, Akbar Bakhtiar, M. Taher
author_sort	Saad, Shahbudin
title	Antioxidant properties of soft coral Dendronephthya sp
title_short	Antioxidant properties of soft coral Dendronephthya sp
title_full	Antioxidant properties of soft coral Dendronephthya sp
title_fullStr	Antioxidant properties of soft coral Dendronephthya sp
title_full_unstemmed	Antioxidant properties of soft coral Dendronephthya sp
title_sort	antioxidant properties of soft coral dendronephthya sp
publisher	A N S I Network
publishDate	2011
url	http://irep.iium.edu.my/4303/ http://irep.iium.edu.my/4303/ http://irep.iium.edu.my/4303/1/International_journal_of_pharmacology.pdf
first_indexed	2023-09-18T20:12:28Z
last_indexed	2023-09-18T20:12:28Z
_version_	1777407569598349312

Antioxidant properties of soft coral Dendronephthya sp

Similar Items