Constitutive, institutive and up-regulation of carotenogenesis regulatory mechanism via in vitro culture model system and elicitors
Every single step in the pathway of carotenogenesis in the leaves or any part of the plants is regulated and controlled by specific genes. It was inferred that these genetic components would be inherited by either undifferentiated (callus) or differentiated (in vitro shoot) cells since they were i...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/42789/ http://irep.iium.edu.my/42789/ http://irep.iium.edu.my/42789/1/Constitutive%2C_instuitive_and_up-regulation-NATRO_5.pdf |
| Summary: | Every single step in the pathway of carotenogenesis in the leaves or any part of the plants is regulated and
controlled by specific genes. It was inferred that these genetic components would be inherited by either
undifferentiated (callus) or differentiated (in vitro shoot) cells since they were initiated from their respective intact
plants. Thus, the in vitro cultures are most likely to develop and respond similarly upon various internal and external
stimuli like the intact plants. Therefore, this study was aimed at establishing in vitro cultures as potential research
tool to study the regulatory mechanism of the carotenoid biosynthesis in medicinal species through a controlled
environment. This establishment would be beneficial as researchers in this field could overcome problems
encountered in researches using intact plants. Via HPLC analysis, it was found that different types of cultures
accumulated different types of carotenoid as compared to their intact plants, accordingly. The results obtained in this
study suggested that the key factors which involved in regulatory mechanisms of individual carotenoid biosynthesis
in a particular biology system of plants can be assessed and predicted. On top of that, further exploration and
manipulation of the environmental conditions is made possible for enhancement or enrichment of certain carotenoid
of interest in food crops without altering the genes and thus issues regarding the introduction of genetically modified
(GM) crops into natural environment and acceptability of consumers towards GM crops could be minimized. |
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