An S188V mutation alters substrate specificity of non-stereospecific α-haloalkanoic acid dehalogenase E (DehE)
The non-stereospecific α-haloalkanoic acid dehalogenase E (DehE) degrades many halo- genated compounds but is ineffective against β-halogenated compounds such as 3- chloropropionic acid (3CP). Using molecular dynamics (MD) simulations and site-directed mutagenesis we show here that introducing th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Public Library of Science (PLoS)
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/42599/ http://irep.iium.edu.my/42599/ http://irep.iium.edu.my/42599/ http://irep.iium.edu.my/42599/1/42599.pdf |