Recombinant enzymes—from basic science to commercialization
on the productivity, efficiency, durability and its applications have been conducted by many researchers worldwide. However, suitable and focus research on a larger scale production is rarely discussed in detail because it is kept as secret by giant companies producing recombinant enzyme. Often t...
| Other Authors: | |
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| Format: | Book |
| Language: | English |
| Published: |
Springer International Publishing
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/42328/ http://irep.iium.edu.my/42328/1/Recombinant_Enzyme.pdf |
| Summary: | on the productivity, efficiency, durability and its applications have been conducted
by many researchers worldwide. However, suitable and focus research on a larger
scale production is rarely discussed in detail because it is kept as secret by giant
companies producing recombinant enzyme. Often the good findings on the latest
discoveries in the laboratory were put on shelf and not suitable to be commercialized.
Therefore, this book aims to share basic science that should be emphasized
by the researchers so that their bench scale results will translates into a commercial
product in the future.
In the first chapter, the reader will be introduced to the recombinant enzyme and
why it is so important in our daily lives. This chapter is also discussing the importance
of research and commercialization strategies. The second chapter highlights
the recombinant DNA strategies in producing recombinant enzyme clone.
The fourth and fifth chapters discuss in more detailed studies that need to be
carried out on the recombinant enzyme especially on the characterization and purification
stages. When the basic rules have been strengthened, research methods in
the up-stream processing of fermentation condition including media formulation
that is able to produce high productivity is discusses in the fifth chapter, followed
by strategies to scale up the production volume to higher scale is discuss in Chap. 7.
Later, Chap. 8 discuss the downstream processing of recombinant enzyme. In
this chapter there are four stages of processes that need to carry out to ensure that
the resulting recombinant enzyme has high quality and purity. To ensure a product
is suitable to be produced in large scale manufacturing plant, engineering design is
required. Chapter nine highlights procedure on how to produce engineering design
along with appropriate software. The important of economic and environmental
evaluation analysis carried out by the software are also discussed.
Finally, Chaps. 10, 11 and 12 bring readers to the case study of recombinant bromelain.
This enzyme has been cloned by recombinant DNA methods and has gone
through phases of many research stages those discussed in chapters two to nine.
Finally, this book would not be a reality if there are no supports from all parties
involved, namely editors, authors and publisher. We hope this book will encourage
researchers to keep continue their basic research related to recombinant enzymeand finally able to commercialize it. This book is dedicated to all recombinant
enzyme researchers especially those who are in Malaysia. Editor dreams that one
day Malaysia will have our own recombinant enzyme manufacturing plant where
many students and biochemical engineer will be able to practice their knowledge. |
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