Host-specific microbial communities in three sympatric North Sea sponges

The establishment of next-generation technology sequencing has deepened our knowledge of marine sponge-associated microbiota with the identification of at least 32 phyla of Bacteria and Archaea from a large number of sponge species. In this study, we assessed the diversity of the microbial communiti...

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Main Authors:	Naim, Mohd Azrul, Morillo, Jose A., Sørensen, Søren J., Waleed, Abu Al-Soud, Smidt, Hauke, Sipkema, Detmer
Format:	Article
Language:	English English
Published:	John Wiley & Sons Ltd. 2014
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/42287/ http://irep.iium.edu.my/42287/ http://irep.iium.edu.my/42287/ http://irep.iium.edu.my/42287/1/fem12400.pdf http://irep.iium.edu.my/42287/4/42287_Host-specific%20microbial%20communities.SCOPUSpdf.pdf

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spelling	iium-422872018-06-11T02:59:54Z http://irep.iium.edu.my/42287/ Host-specific microbial communities in three sympatric North Sea sponges Naim, Mohd Azrul Morillo, Jose A. Sørensen, Søren J. Waleed, Abu Al-Soud Smidt, Hauke Sipkema, Detmer QR Microbiology The establishment of next-generation technology sequencing has deepened our knowledge of marine sponge-associated microbiota with the identification of at least 32 phyla of Bacteria and Archaea from a large number of sponge species. In this study, we assessed the diversity of the microbial communities hosted by three sympatric sponges living in a semi-enclosed North Sea environment using pyrosequencing of bacterial and archaeal 16S ribosomal RNA gene fragments. The three sponges harbor species-specific communities each dominated by a different class of Proteobacteria. An -proteobacterial Rhodobacter-like phylotype was confirmed as the predominant symbiont of Halichondria panicea. The microbial communities of Haliclona xena and H.oculata are described for the first time in this study and are dominated by Gammaproteobacteria and Betaproteobacteria, respectively. Several common phylotypes belonging to Chlamydiae, TM6, Actinobacteria, and Betaproteobacteria were detected in all sponge samples. A number of phylotypes of the phylum Chlamydiae were present at an unprecedentedly high relative abundance of up to 14.4 +/- 1.4% of the total reads, which suggests an important ecological role in North Sea sponges. These Chlamydiae-affiliated operational taxonomic units may represent novel lineages at least at the genus level as they are only 86-92% similar to known sequences. John Wiley & Sons Ltd. 2014-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/42287/1/fem12400.pdf application/pdf en http://irep.iium.edu.my/42287/4/42287_Host-specific%20microbial%20communities.SCOPUSpdf.pdf Naim, Mohd Azrul and Morillo, Jose A. and Sørensen, Søren J. and Waleed, Abu Al-Soud and Smidt, Hauke and Sipkema, Detmer (2014) Host-specific microbial communities in three sympatric North Sea sponges. FEMS Microbiology Ecology, 90 (2). pp. 390-403. ISSN 0168-6496 http://femsec.oxfordjournals.org/content/90/2/390 10.1111/1574-6941.12400
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Naim, Mohd Azrul Morillo, Jose A. Sørensen, Søren J. Waleed, Abu Al-Soud Smidt, Hauke Sipkema, Detmer Host-specific microbial communities in three sympatric North Sea sponges
description	The establishment of next-generation technology sequencing has deepened our knowledge of marine sponge-associated microbiota with the identification of at least 32 phyla of Bacteria and Archaea from a large number of sponge species. In this study, we assessed the diversity of the microbial communities hosted by three sympatric sponges living in a semi-enclosed North Sea environment using pyrosequencing of bacterial and archaeal 16S ribosomal RNA gene fragments. The three sponges harbor species-specific communities each dominated by a different class of Proteobacteria. An -proteobacterial Rhodobacter-like phylotype was confirmed as the predominant symbiont of Halichondria panicea. The microbial communities of Haliclona xena and H.oculata are described for the first time in this study and are dominated by Gammaproteobacteria and Betaproteobacteria, respectively. Several common phylotypes belonging to Chlamydiae, TM6, Actinobacteria, and Betaproteobacteria were detected in all sponge samples. A number of phylotypes of the phylum Chlamydiae were present at an unprecedentedly high relative abundance of up to 14.4 +/- 1.4% of the total reads, which suggests an important ecological role in North Sea sponges. These Chlamydiae-affiliated operational taxonomic units may represent novel lineages at least at the genus level as they are only 86-92% similar to known sequences.
format	Article
author	Naim, Mohd Azrul Morillo, Jose A. Sørensen, Søren J. Waleed, Abu Al-Soud Smidt, Hauke Sipkema, Detmer
author_facet	Naim, Mohd Azrul Morillo, Jose A. Sørensen, Søren J. Waleed, Abu Al-Soud Smidt, Hauke Sipkema, Detmer
author_sort	Naim, Mohd Azrul
title	Host-specific microbial communities in three sympatric North Sea sponges
title_short	Host-specific microbial communities in three sympatric North Sea sponges
title_full	Host-specific microbial communities in three sympatric North Sea sponges
title_fullStr	Host-specific microbial communities in three sympatric North Sea sponges
title_full_unstemmed	Host-specific microbial communities in three sympatric North Sea sponges
title_sort	host-specific microbial communities in three sympatric north sea sponges
publisher	John Wiley & Sons Ltd.
publishDate	2014
url	http://irep.iium.edu.my/42287/ http://irep.iium.edu.my/42287/ http://irep.iium.edu.my/42287/ http://irep.iium.edu.my/42287/1/fem12400.pdf http://irep.iium.edu.my/42287/4/42287_Host-specific%20microbial%20communities.SCOPUSpdf.pdf
first_indexed	2023-09-18T21:00:18Z
last_indexed	2023-09-18T21:00:18Z
_version_	1777410578847891456

Host-specific microbial communities in three sympatric North Sea sponges

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