Endo sensor bacterial endotoxin detector in liquid biological samples

Endotoxins (Lipopolysaccharide) from gram-negative microorganisms initiates clot formation in blood when it is accidentally encountered by horseshoe crab blood stream. This property was extensively studied by various researchers as a result Limulus Amebocyte Lysate (LAL) test was established. The LA...

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Main Authors:	John, Akbar, Yunus, Kamaruzzaman, Khan Chowdhury, Ahmed Jalal, Kamaruddin, Zaleha
Format:	Conference or Workshop Item
Language:	English English
Published:	2011
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/4219/ http://irep.iium.edu.my/4219/1/Endo_sensor_ppt_IIUM_congress_2011.pptx http://irep.iium.edu.my/4219/2/Endo_sensor-_bacterial_endotoxin_detector_in_liquid_biological_samples.pdf

id	iium-4219
recordtype	eprints
spelling	iium-42192017-12-06T05:02:53Z http://irep.iium.edu.my/4219/ Endo sensor bacterial endotoxin detector in liquid biological samples John, Akbar Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Kamaruddin, Zaleha QL Zoology Endotoxins (Lipopolysaccharide) from gram-negative microorganisms initiates clot formation in blood when it is accidentally encountered by horseshoe crab blood stream. This property was extensively studied by various researchers as a result Limulus Amebocyte Lysate (LAL) test was established. The LAL tests in general, 3 to 300 times more sensitive than the United States Pharmacopeial (USP) rabbit pyrogen test method. It is apparent that major differences among the LAL preparations lie in the area of sensitivity. Differences, up to 100-fold, exist in the sensitivity of the various LAL preparations to the same endotoxin. Based on above perspective a portable Kit (Endo sensor) was developed to detect the presence of bacterial endotoxin in liquid biological samples using Tachypleus Amebocyte Lysate (TAL) as a source. Sensitivity of the Kit was determined using various concentrations of prepared endotoxin standards and pyrogen free water samples. It was observed that Endo sensor could detect up to nano gram level of endotoxin in liquid biological samples which could be expressed in (EU/ml) and the labeled sensitivity of the lysated product was 0.125 EU/ml. The gel clotting principle method was utilized for the detection of bacterial endotoxin in liquid biological samples. 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/4219/1/Endo_sensor_ppt_IIUM_congress_2011.pptx application/pdf en http://irep.iium.edu.my/4219/2/Endo_sensor-_bacterial_endotoxin_detector_in_liquid_biological_samples.pdf John, Akbar and Yunus, Kamaruzzaman and Khan Chowdhury, Ahmed Jalal and Kamaruddin, Zaleha (2011) Endo sensor bacterial endotoxin detector in liquid biological samples. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QL Zoology
spellingShingle	QL Zoology John, Akbar Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Kamaruddin, Zaleha Endo sensor bacterial endotoxin detector in liquid biological samples
description	Endotoxins (Lipopolysaccharide) from gram-negative microorganisms initiates clot formation in blood when it is accidentally encountered by horseshoe crab blood stream. This property was extensively studied by various researchers as a result Limulus Amebocyte Lysate (LAL) test was established. The LAL tests in general, 3 to 300 times more sensitive than the United States Pharmacopeial (USP) rabbit pyrogen test method. It is apparent that major differences among the LAL preparations lie in the area of sensitivity. Differences, up to 100-fold, exist in the sensitivity of the various LAL preparations to the same endotoxin. Based on above perspective a portable Kit (Endo sensor) was developed to detect the presence of bacterial endotoxin in liquid biological samples using Tachypleus Amebocyte Lysate (TAL) as a source. Sensitivity of the Kit was determined using various concentrations of prepared endotoxin standards and pyrogen free water samples. It was observed that Endo sensor could detect up to nano gram level of endotoxin in liquid biological samples which could be expressed in (EU/ml) and the labeled sensitivity of the lysated product was 0.125 EU/ml. The gel clotting principle method was utilized for the detection of bacterial endotoxin in liquid biological samples.
format	Conference or Workshop Item
author	John, Akbar Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Kamaruddin, Zaleha
author_facet	John, Akbar Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Kamaruddin, Zaleha
author_sort	John, Akbar
title	Endo sensor bacterial endotoxin detector in liquid biological samples
title_short	Endo sensor bacterial endotoxin detector in liquid biological samples
title_full	Endo sensor bacterial endotoxin detector in liquid biological samples
title_fullStr	Endo sensor bacterial endotoxin detector in liquid biological samples
title_full_unstemmed	Endo sensor bacterial endotoxin detector in liquid biological samples
title_sort	endo sensor bacterial endotoxin detector in liquid biological samples
publishDate	2011
url	http://irep.iium.edu.my/4219/ http://irep.iium.edu.my/4219/1/Endo_sensor_ppt_IIUM_congress_2011.pptx http://irep.iium.edu.my/4219/2/Endo_sensor-_bacterial_endotoxin_detector_in_liquid_biological_samples.pdf
first_indexed	2023-09-18T20:12:21Z
last_indexed	2023-09-18T20:12:21Z
_version_	1777407562137731072

Endo sensor bacterial endotoxin detector in liquid biological samples

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