DNA barcoding of Lates calcarifer (Bloch, 1970)

An effort to assess the utility of 650 bp Cytochrome oxidase subunit I (DNA barcode) gene in delineating the members closely resembled marine fin fishes belonging to the family Latidae was made. To address the issue, all 40 barcode sequences of fishes belonging to Latidae family available at NCBI (N...

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Main Authors:	John, Akbar, Kumar, C. Prasanna, Lyla, P.S., Khan, S. Ajmal, Khan Chowdhury, Ahmed Jalal
Format:	Article
Language:	English
Published:	Medwell Journals 2010
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/4213/ http://irep.iium.edu.my/4213/ http://irep.iium.edu.my/4213/ http://irep.iium.edu.my/4213/1/414-419.pdf

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recordtype	eprints
spelling	iium-42132017-12-07T05:06:49Z http://irep.iium.edu.my/4213/ DNA barcoding of Lates calcarifer (Bloch, 1970) John, Akbar Kumar, C. Prasanna Lyla, P.S. Khan, S. Ajmal Khan Chowdhury, Ahmed Jalal QL Zoology An effort to assess the utility of 650 bp Cytochrome oxidase subunit I (DNA barcode) gene in delineating the members closely resembled marine fin fishes belonging to the family Latidae was made. To address the issue, all 40 barcode sequences of fishes belonging to Latidae family available at NCBI (National Centre for Biotechnological Information) along with the barcode data generated from commonly available fish Asian sea bass (Lates calcarifer) from Parangipettai coastal waters was used. The average GC content of selected three fishes belongs to Latidae was found to be 48.87%. Lates niloticus from Tanzanian waters showed less GC content (47.70%) whereas L. calcarifer from South China sea showed high GC content (49.61%) among the Latidae species studied. Compare to Australian, Indian and Singapore (48.1-48.89%) water L. calcarifer, Myanmar and South China species shows high average GC content (49.38-49.41%). The phylogenetic and genetic distance data showed that the maximum genetic distance is present in 3rd codon position in all the selected species. It is apparent from the genetic distance that the GD between L. calcarifer and L. niloticus was observed to be higher than the GD between P. waigiensis and L. niloticus in all the codon positions. The expected clustering of the species in separate internal branches was well observed in the constructed phylogram with high bootstraps. We conclude that COI sequencing (barcoding) in identifying the morphologically similar species of the family Latidae. Medwell Journals 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/4213/1/414-419.pdf John, Akbar and Kumar, C. Prasanna and Lyla, P.S. and Khan, S. Ajmal and Khan Chowdhury, Ahmed Jalal (2010) DNA barcoding of Lates calcarifer (Bloch, 1970). Research Journal of Biological Sciences, 5 (6). pp. 414-419. ISSN 1815-8846 (P), 1993-6087 (O) http://medwelljournals.com/abstract/?doi=rjbsci.2010.414.419 10.3923/rjbsci.2010.414.419
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QL Zoology
spellingShingle	QL Zoology John, Akbar Kumar, C. Prasanna Lyla, P.S. Khan, S. Ajmal Khan Chowdhury, Ahmed Jalal DNA barcoding of Lates calcarifer (Bloch, 1970)
description	An effort to assess the utility of 650 bp Cytochrome oxidase subunit I (DNA barcode) gene in delineating the members closely resembled marine fin fishes belonging to the family Latidae was made. To address the issue, all 40 barcode sequences of fishes belonging to Latidae family available at NCBI (National Centre for Biotechnological Information) along with the barcode data generated from commonly available fish Asian sea bass (Lates calcarifer) from Parangipettai coastal waters was used. The average GC content of selected three fishes belongs to Latidae was found to be 48.87%. Lates niloticus from Tanzanian waters showed less GC content (47.70%) whereas L. calcarifer from South China sea showed high GC content (49.61%) among the Latidae species studied. Compare to Australian, Indian and Singapore (48.1-48.89%) water L. calcarifer, Myanmar and South China species shows high average GC content (49.38-49.41%). The phylogenetic and genetic distance data showed that the maximum genetic distance is present in 3rd codon position in all the selected species. It is apparent from the genetic distance that the GD between L. calcarifer and L. niloticus was observed to be higher than the GD between P. waigiensis and L. niloticus in all the codon positions. The expected clustering of the species in separate internal branches was well observed in the constructed phylogram with high bootstraps. We conclude that COI sequencing (barcoding) in identifying the morphologically similar species of the family Latidae.
format	Article
author	John, Akbar Kumar, C. Prasanna Lyla, P.S. Khan, S. Ajmal Khan Chowdhury, Ahmed Jalal
author_facet	John, Akbar Kumar, C. Prasanna Lyla, P.S. Khan, S. Ajmal Khan Chowdhury, Ahmed Jalal
author_sort	John, Akbar
title	DNA barcoding of Lates calcarifer (Bloch, 1970)
title_short	DNA barcoding of Lates calcarifer (Bloch, 1970)
title_full	DNA barcoding of Lates calcarifer (Bloch, 1970)
title_fullStr	DNA barcoding of Lates calcarifer (Bloch, 1970)
title_full_unstemmed	DNA barcoding of Lates calcarifer (Bloch, 1970)
title_sort	dna barcoding of lates calcarifer (bloch, 1970)
publisher	Medwell Journals
publishDate	2010
url	http://irep.iium.edu.my/4213/ http://irep.iium.edu.my/4213/ http://irep.iium.edu.my/4213/ http://irep.iium.edu.my/4213/1/414-419.pdf
first_indexed	2023-09-18T20:12:20Z
last_indexed	2023-09-18T20:12:20Z
_version_	1777407561311453184

DNA barcoding of Lates calcarifer (Bloch, 1970)

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