Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum

Tissue engineering is a new biotechnological field that has emerged recently, providing an alternative to the treatment of damages and defects of human tissues. Amongst the most researched tissues is cartilage. In this study, the general characteristics of chondrocytes derived from three different...

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Main Authors:	Idrus, Ruszymah, Chua, Kien Hui, Sha'ban, Munirah, Ahmad Noruddin, Nur Adelina, Saim, Aminuddin
Format:	Article
Language:	English
Published:	Islamic World Academy of Sciences 2005
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41877/ http://irep.iium.edu.my/41877/ http://irep.iium.edu.my/41877/1/IdrusWCHMRKPUAE60185.pdf

id	iium-41877
recordtype	eprints
spelling	iium-418772015-06-29T04:39:02Z http://irep.iium.edu.my/41877/ Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum Idrus, Ruszymah Chua, Kien Hui Sha'ban, Munirah Ahmad Noruddin, Nur Adelina Saim, Aminuddin R Medicine (General) Tissue engineering is a new biotechnological field that has emerged recently, providing an alternative to the treatment of damages and defects of human tissues. Amongst the most researched tissues is cartilage. In this study, the general characteristics of chondrocytes derived from three different sources in the body: articular, auricular and nasal septum cartilage, were investigated, looking both at cellular and tissue construct levels. With the approval of Research and Ethical Committee of Medical Faculty, Universiti Kebangsaan Malaysia, redundant cartilages from human articular, auricular and nasal septum were obtained from several patients during surgical procedures. Samples were digested and chondrocytes obtained were cultured in a mixture of Ham's F12 : DMEM medium. All three chondrocyte sources were found to behave quite similarly in terms of cell morphology, gene expressions and histological analysis. No significant difference of the growth rates was shown by the three chondrocyte sources. Histological analysis via H and E and Safranin O stainings of the tissue-engineered cartilages demonstrate resemblance to the native cartilage. Gene expression results showed that collagen type II expression reduces after every passage, and collagen type I gene is expressed as early as P0, however, there were re-expressions of Collagen Type II gene in the in vivo construct. We have successfully engineered cartilage tissues which are similar to the native cartilage, using sources from articular, auricular and nasal septum chondrocytes. Islamic World Academy of Sciences 2005 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41877/1/IdrusWCHMRKPUAE60185.pdf Idrus, Ruszymah and Chua, Kien Hui and Sha'ban, Munirah and Ahmad Noruddin, Nur Adelina and Saim, Aminuddin (2005) Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum. Medical Journal of Islamic World Academy of Sciences, 15 (1). pp. 5-12. ISSN 1016-3360 http://www.medicaljournal-ias.org/tr/Icerik.aspx?IcerikID=102
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Idrus, Ruszymah Chua, Kien Hui Sha'ban, Munirah Ahmad Noruddin, Nur Adelina Saim, Aminuddin Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum
description	Tissue engineering is a new biotechnological field that has emerged recently, providing an alternative to the treatment of damages and defects of human tissues. Amongst the most researched tissues is cartilage. In this study, the general characteristics of chondrocytes derived from three different sources in the body: articular, auricular and nasal septum cartilage, were investigated, looking both at cellular and tissue construct levels. With the approval of Research and Ethical Committee of Medical Faculty, Universiti Kebangsaan Malaysia, redundant cartilages from human articular, auricular and nasal septum were obtained from several patients during surgical procedures. Samples were digested and chondrocytes obtained were cultured in a mixture of Ham's F12 : DMEM medium. All three chondrocyte sources were found to behave quite similarly in terms of cell morphology, gene expressions and histological analysis. No significant difference of the growth rates was shown by the three chondrocyte sources. Histological analysis via H and E and Safranin O stainings of the tissue-engineered cartilages demonstrate resemblance to the native cartilage. Gene expression results showed that collagen type II expression reduces after every passage, and collagen type I gene is expressed as early as P0, however, there were re-expressions of Collagen Type II gene in the in vivo construct. We have successfully engineered cartilage tissues which are similar to the native cartilage, using sources from articular, auricular and nasal septum chondrocytes.
format	Article
author	Idrus, Ruszymah Chua, Kien Hui Sha'ban, Munirah Ahmad Noruddin, Nur Adelina Saim, Aminuddin
author_facet	Idrus, Ruszymah Chua, Kien Hui Sha'ban, Munirah Ahmad Noruddin, Nur Adelina Saim, Aminuddin
author_sort	Idrus, Ruszymah
title	Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum
title_short	Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum
title_full	Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum
title_fullStr	Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum
title_full_unstemmed	Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum
title_sort	tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum
publisher	Islamic World Academy of Sciences
publishDate	2005
url	http://irep.iium.edu.my/41877/ http://irep.iium.edu.my/41877/ http://irep.iium.edu.my/41877/1/IdrusWCHMRKPUAE60185.pdf
first_indexed	2023-09-18T20:59:50Z
last_indexed	2023-09-18T20:59:50Z
_version_	1777410549281193984

Tissue engineered cartilage with different human chondrocyte sources: articular, auricular and nasal septum

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