Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage

Objectives: This study was aimed at regenerating autologous elastic cartilage for future use in pediatric ear reconstruction surgery. Specific attentions were to characterize pediatric auricular chondrocyte growth in a combination culture medium and to assess the possibility of elastic cartilage re...

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Main Authors:	Idrus, Ruszymah, Saim, Lokman, Abdullah, Asma, Sha'ban, Munirah, Chua, Kien Hui, Abdul Latif, Mazlyzam, M. R., Isa, Nor Hussien, Fuzina, Saim, Aminuddin
Format:	Article
Language:	English
Published:	Elsevier B.V. 2007
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41866/ http://irep.iium.edu.my/41866/ http://irep.iium.edu.my/41866/1/INT_PAED_ORL_MAY_2007.pdf

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spelling	iium-418662015-04-17T07:18:52Z http://irep.iium.edu.my/41866/ Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage Idrus, Ruszymah Saim, Lokman Abdullah, Asma Sha'ban, Munirah Chua, Kien Hui Abdul Latif, Mazlyzam M. R., Isa Nor Hussien, Fuzina Saim, Aminuddin R Medicine (General) Objectives: This study was aimed at regenerating autologous elastic cartilage for future use in pediatric ear reconstruction surgery. Specific attentions were to characterize pediatric auricular chondrocyte growth in a combination culture medium and to assess the possibility of elastic cartilage regeneration using human fibrin. Study design: Laboratory experiment using human pediatric auricular chondrocytes.Methods: Pediatric auricular chondrocytes growth kinetics and quantitative gene expression profile in three different types of media were compared in primary culture and subsequent three passages. Large-scale culture-expanded chondrocytes from the combination medium were then mixed with human fibrin for the formation of elastic cartilage via tissue engineering technique. Results: The equal mixture of Ham’s F12 and Dulbecco’s Modified Eagle Medium (FD) promoted the best chondrocyte growth at every passage compared to the individual media. Chondrocytes differentiation index; ratio of type II to type I collagen gene expression level, aggrecan and elastin expression gradually decreased while passaging but they were then restored in engineered tissues after implantation. The engineered cartilage was glistening white in color and firm in consistency. Histological evaluation, immunohistochemistry analysis and quantitative gene expression assessment demonstrated that the engineered cartilage resemble the features of native elastic cartilage. Conclusion: Pediatric auricular chondrocytes proliferate better in the combination medium (FD) and the utilization of human fibrin as a biomaterial hold promises for the regeneration of an autologous elastic cartilage for future application in ear reconstructive surgery. Elsevier B.V. 2007 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41866/1/INT_PAED_ORL_MAY_2007.pdf Idrus, Ruszymah and Saim, Lokman and Abdullah, Asma and Sha'ban, Munirah and Chua, Kien Hui and Abdul Latif, Mazlyzam and M. R., Isa and Nor Hussien, Fuzina and Saim, Aminuddin (2007) Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage. International Journal of Pediatric Otorhinolaryngology , 71 (8). pp. 1225-1234. ISSN 0165-5876 http://www.journals.elsevier.com/international-journal-of-pediatric-otorhinolaryngology/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Idrus, Ruszymah Saim, Lokman Abdullah, Asma Sha'ban, Munirah Chua, Kien Hui Abdul Latif, Mazlyzam M. R., Isa Nor Hussien, Fuzina Saim, Aminuddin Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage
description	Objectives: This study was aimed at regenerating autologous elastic cartilage for future use in pediatric ear reconstruction surgery. Specific attentions were to characterize pediatric auricular chondrocyte growth in a combination culture medium and to assess the possibility of elastic cartilage regeneration using human fibrin. Study design: Laboratory experiment using human pediatric auricular chondrocytes.Methods: Pediatric auricular chondrocytes growth kinetics and quantitative gene expression profile in three different types of media were compared in primary culture and subsequent three passages. Large-scale culture-expanded chondrocytes from the combination medium were then mixed with human fibrin for the formation of elastic cartilage via tissue engineering technique. Results: The equal mixture of Ham’s F12 and Dulbecco’s Modified Eagle Medium (FD) promoted the best chondrocyte growth at every passage compared to the individual media. Chondrocytes differentiation index; ratio of type II to type I collagen gene expression level, aggrecan and elastin expression gradually decreased while passaging but they were then restored in engineered tissues after implantation. The engineered cartilage was glistening white in color and firm in consistency. Histological evaluation, immunohistochemistry analysis and quantitative gene expression assessment demonstrated that the engineered cartilage resemble the features of native elastic cartilage. Conclusion: Pediatric auricular chondrocytes proliferate better in the combination medium (FD) and the utilization of human fibrin as a biomaterial hold promises for the regeneration of an autologous elastic cartilage for future application in ear reconstructive surgery.
format	Article
author	Idrus, Ruszymah Saim, Lokman Abdullah, Asma Sha'ban, Munirah Chua, Kien Hui Abdul Latif, Mazlyzam M. R., Isa Nor Hussien, Fuzina Saim, Aminuddin
author_facet	Idrus, Ruszymah Saim, Lokman Abdullah, Asma Sha'ban, Munirah Chua, Kien Hui Abdul Latif, Mazlyzam M. R., Isa Nor Hussien, Fuzina Saim, Aminuddin
author_sort	Idrus, Ruszymah
title	Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage
title_short	Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage
title_full	Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage
title_fullStr	Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage
title_full_unstemmed	Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage
title_sort	pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage
publisher	Elsevier B.V.
publishDate	2007
url	http://irep.iium.edu.my/41866/ http://irep.iium.edu.my/41866/ http://irep.iium.edu.my/41866/1/INT_PAED_ORL_MAY_2007.pdf
first_indexed	2023-09-18T20:59:49Z
last_indexed	2023-09-18T20:59:49Z
_version_	1777410548271415296

Pediatric auricular chondrocytes gene expression analysis in monolayer culture and engineered elastic cartilage

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