Autologous versus pooled human serum for articular chondrocyte growth

Purpose. To evaluate the effect of autologous human serum (AHS) versus pooled human serum (PHS) versus foetal bovine serum (FBS) for growth of articular chondrocytes and formation of chondrocytefibrin constructs. Methods. Experiments with monolayer culture expansion of human articular chondroc...

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Main Authors:	Sha'ban, Munirah, Idrus, Ruszymah, Osman Cassim, Samsudin, M. Yusuf, Badrul Akmal Hisham, Baharudin, Azmi, Saim, Aminuddin
Format:	Article
Language:	English
Published:	BioMed Central Ltd. 2008
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41837/ http://irep.iium.edu.my/41837/ http://irep.iium.edu.my/41837/1/AHS_versus_PHS_2008_MUNIRAH_ET_AL.pdf

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spelling	iium-418372015-03-13T03:21:06Z http://irep.iium.edu.my/41837/ Autologous versus pooled human serum for articular chondrocyte growth Sha'ban, Munirah Idrus, Ruszymah Osman Cassim, Samsudin M. Yusuf, Badrul Akmal Hisham Baharudin, Azmi Saim, Aminuddin R Medicine (General) Purpose. To evaluate the effect of autologous human serum (AHS) versus pooled human serum (PHS) versus foetal bovine serum (FBS) for growth of articular chondrocytes and formation of chondrocytefibrin constructs. Methods. Experiments with monolayer culture expansion of human articular chondrocytes were performed using basic culture media supplemented with 10% AHS, PHS, or FBS. Growth kinetics and specific phenotypic expression of the serially expanded chondrocytes were evaluated. Largescale culture expansion was used to obtain about 30 million cells to form chondrocyte-fibrin constructs. All constructs were implanted subcutaneously at the dorsum part of athymic nude mice for 8 weeks. The in vivo constructs were evaluated using histological and gene expression studies. Results. The morphology of primary cultured chondrocytes (P0) was polygonal and became more elongated and larger after serial passages (P1, P2, and P3). This was comparable for AHS, PHS, and FBS. Total cell yields accumulated for AHS (28 million) and PHS (41 million) were significantly higher than those for FBS (4 million). After 8 weeks of implantation, in vivo chondrocyte-fibrin constructs demonstrated a glistening white and firm texture, comparable to normal hyaline cartilage. All constructs exhibited histo-architectural characteristics of well-distributed cartilage-isolated cells embedded within basophilic ground substance. Presence of accumulated proteoglycans cartilage-rich matrix was indicated by positive orange-red Safranin O staining. During monolayer culture expansion, collagen type II gene expression was down-regulated, while collagen type I gene expression was up-regulated. Collagen type II—the specific chondrogenesis marker—was reexpressed in the in vivo chondrocyte-fibrin construct. Conclusion. AHS and PHS are better than FBS for in vitro cultivation of human articular chondrocytes. BioMed Central Ltd. 2008 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41837/1/AHS_versus_PHS_2008_MUNIRAH_ET_AL.pdf Sha'ban, Munirah and Idrus, Ruszymah and Osman Cassim, Samsudin and M. Yusuf, Badrul Akmal Hisham and Baharudin, Azmi and Saim, Aminuddin (2008) Autologous versus pooled human serum for articular chondrocyte growth. Journal of Orthopaedic Surgery and Research, 16 (2). pp. 220-229. ISSN 1749-799X http://www.josonline.org/index.php/JOS
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Sha'ban, Munirah Idrus, Ruszymah Osman Cassim, Samsudin M. Yusuf, Badrul Akmal Hisham Baharudin, Azmi Saim, Aminuddin Autologous versus pooled human serum for articular chondrocyte growth
description	Purpose. To evaluate the effect of autologous human serum (AHS) versus pooled human serum (PHS) versus foetal bovine serum (FBS) for growth of articular chondrocytes and formation of chondrocytefibrin constructs. Methods. Experiments with monolayer culture expansion of human articular chondrocytes were performed using basic culture media supplemented with 10% AHS, PHS, or FBS. Growth kinetics and specific phenotypic expression of the serially expanded chondrocytes were evaluated. Largescale culture expansion was used to obtain about 30 million cells to form chondrocyte-fibrin constructs. All constructs were implanted subcutaneously at the dorsum part of athymic nude mice for 8 weeks. The in vivo constructs were evaluated using histological and gene expression studies. Results. The morphology of primary cultured chondrocytes (P0) was polygonal and became more elongated and larger after serial passages (P1, P2, and P3). This was comparable for AHS, PHS, and FBS. Total cell yields accumulated for AHS (28 million) and PHS (41 million) were significantly higher than those for FBS (4 million). After 8 weeks of implantation, in vivo chondrocyte-fibrin constructs demonstrated a glistening white and firm texture, comparable to normal hyaline cartilage. All constructs exhibited histo-architectural characteristics of well-distributed cartilage-isolated cells embedded within basophilic ground substance. Presence of accumulated proteoglycans cartilage-rich matrix was indicated by positive orange-red Safranin O staining. During monolayer culture expansion, collagen type II gene expression was down-regulated, while collagen type I gene expression was up-regulated. Collagen type II—the specific chondrogenesis marker—was reexpressed in the in vivo chondrocyte-fibrin construct. Conclusion. AHS and PHS are better than FBS for in vitro cultivation of human articular chondrocytes.
format	Article
author	Sha'ban, Munirah Idrus, Ruszymah Osman Cassim, Samsudin M. Yusuf, Badrul Akmal Hisham Baharudin, Azmi Saim, Aminuddin
author_facet	Sha'ban, Munirah Idrus, Ruszymah Osman Cassim, Samsudin M. Yusuf, Badrul Akmal Hisham Baharudin, Azmi Saim, Aminuddin
author_sort	Sha'ban, Munirah
title	Autologous versus pooled human serum for articular chondrocyte growth
title_short	Autologous versus pooled human serum for articular chondrocyte growth
title_full	Autologous versus pooled human serum for articular chondrocyte growth
title_fullStr	Autologous versus pooled human serum for articular chondrocyte growth
title_full_unstemmed	Autologous versus pooled human serum for articular chondrocyte growth
title_sort	autologous versus pooled human serum for articular chondrocyte growth
publisher	BioMed Central Ltd.
publishDate	2008
url	http://irep.iium.edu.my/41837/ http://irep.iium.edu.my/41837/ http://irep.iium.edu.my/41837/1/AHS_versus_PHS_2008_MUNIRAH_ET_AL.pdf
first_indexed	2023-09-18T20:59:46Z
last_indexed	2023-09-18T20:59:46Z
_version_	1777410545508417536

Autologous versus pooled human serum for articular chondrocyte growth

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