Exploring three dimensional culture system for cartilage tissue engineering application: a review

Articular cartilage is a unique tissue with limited self-repair ability. The field of articular cartilage tissue engineering has emerged as a significant complementary solution that involves multidisciplinary field and refers to the practice of combining scaffolds, cells, and signalling molecules in...

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Main Authors:	Abdul Rahman, Rozlin, Mohamad Sukri, Norhamiza, Md Nazir, Noorhidayah, Ahmad Radzi, Muhammad Aa'zamuddin, Mohamad, Mohd Yusof, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41804/ http://irep.iium.edu.my/41804/ http://irep.iium.edu.my/41804/1/KAHS_RW2014_ROZLIN.pdf

id	iium-41804
recordtype	eprints
spelling	iium-418042018-06-29T03:11:01Z http://irep.iium.edu.my/41804/ Exploring three dimensional culture system for cartilage tissue engineering application: a review Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Mohamad, Mohd Yusof Sha'ban, Munirah R Medicine (General) Articular cartilage is a unique tissue with limited self-repair ability. The field of articular cartilage tissue engineering has emerged as a significant complementary solution that involves multidisciplinary field and refers to the practice of combining scaffolds, cells, and signalling molecules into functional tissues. The main goal of tissue engineering is to assemble functional constructs or human spare parts that are able to repair, restore, maintain, or improve damaged tissues or whole organs. In mimicking the in vivo microenvironment to resemble physiological conditions, both conventional two-dimensional (2D) and three-dimensional (3D) biomaterial based culture are important in a wide range of cartilage research. Two dimension cell culture techniques have been developed for decades and have become the standard technique of cell culturing across the globe. In the absence of systematic variations from in vivo system, 2D cell culture gives the ability to study the physiological effects of various stimuli, enhancing our understanding of basic cell biology including the cause and effect relationship. However, cell cultured in this system lacks 3D tissue architecture and does not provide a true resemblance of living tissue. The limited proliferative ability and chances of dedifferentiation during in vitro 2D expansion could possibly hinder the chondrocyte and stem cell-based cartilage tissue engineering. This limitation can be overcome by tissue engineering models. Three-dimensional in vitro cartilage models allow the study of cell-cell and cell-extracellular matrix interactions with addition to the influence of the micro environment on cellular differentiation and proliferation. It simulates normal cell morphology, proliferation, differentiation and migrations, better than the 2D cell culture. Dynamic 3D cultures using scaffolds be it natural or synthetic scaffolds are currently being explored. With continuous research, it is hoped that the employment of 3D cultures will provide promising insight into developing effective strategies and successful intervention in managing cartilage disorders. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/41804/1/KAHS_RW2014_ROZLIN.pdf Abdul Rahman, Rozlin and Mohamad Sukri, Norhamiza and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Mohamad, Mohd Yusof and Sha'ban, Munirah (2014) Exploring three dimensional culture system for cartilage tissue engineering application: a review. In: K.A.H.S. Research Week 2014, 17-21 Nov. 2014, KAHS IIUM, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/kahs/events/kahs-research-week-2014
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Mohamad, Mohd Yusof Sha'ban, Munirah Exploring three dimensional culture system for cartilage tissue engineering application: a review
description	Articular cartilage is a unique tissue with limited self-repair ability. The field of articular cartilage tissue engineering has emerged as a significant complementary solution that involves multidisciplinary field and refers to the practice of combining scaffolds, cells, and signalling molecules into functional tissues. The main goal of tissue engineering is to assemble functional constructs or human spare parts that are able to repair, restore, maintain, or improve damaged tissues or whole organs. In mimicking the in vivo microenvironment to resemble physiological conditions, both conventional two-dimensional (2D) and three-dimensional (3D) biomaterial based culture are important in a wide range of cartilage research. Two dimension cell culture techniques have been developed for decades and have become the standard technique of cell culturing across the globe. In the absence of systematic variations from in vivo system, 2D cell culture gives the ability to study the physiological effects of various stimuli, enhancing our understanding of basic cell biology including the cause and effect relationship. However, cell cultured in this system lacks 3D tissue architecture and does not provide a true resemblance of living tissue. The limited proliferative ability and chances of dedifferentiation during in vitro 2D expansion could possibly hinder the chondrocyte and stem cell-based cartilage tissue engineering. This limitation can be overcome by tissue engineering models. Three-dimensional in vitro cartilage models allow the study of cell-cell and cell-extracellular matrix interactions with addition to the influence of the micro environment on cellular differentiation and proliferation. It simulates normal cell morphology, proliferation, differentiation and migrations, better than the 2D cell culture. Dynamic 3D cultures using scaffolds be it natural or synthetic scaffolds are currently being explored. With continuous research, it is hoped that the employment of 3D cultures will provide promising insight into developing effective strategies and successful intervention in managing cartilage disorders.
format	Conference or Workshop Item
author	Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Mohamad, Mohd Yusof Sha'ban, Munirah
author_facet	Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Mohamad, Mohd Yusof Sha'ban, Munirah
author_sort	Abdul Rahman, Rozlin
title	Exploring three dimensional culture system for cartilage tissue engineering application: a review
title_short	Exploring three dimensional culture system for cartilage tissue engineering application: a review
title_full	Exploring three dimensional culture system for cartilage tissue engineering application: a review
title_fullStr	Exploring three dimensional culture system for cartilage tissue engineering application: a review
title_full_unstemmed	Exploring three dimensional culture system for cartilage tissue engineering application: a review
title_sort	exploring three dimensional culture system for cartilage tissue engineering application: a review
publishDate	2014
url	http://irep.iium.edu.my/41804/ http://irep.iium.edu.my/41804/ http://irep.iium.edu.my/41804/1/KAHS_RW2014_ROZLIN.pdf
first_indexed	2023-09-18T20:59:45Z
last_indexed	2023-09-18T20:59:45Z
_version_	1777410543741566976

Exploring three dimensional culture system for cartilage tissue engineering application: a review

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