Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans

Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans

Tetrathionate hydrolase produced by Thiobacillus ferrooxidans has been known to be the enzyme responsible in the devulcanization of used rubber prior to recycling. The aim of this study was to statistically obtain a model that would yield an optimized media concentrations for the best production of...

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Main Authors: Yusof, Faridah, Mohd Dom, Azratul Ashimah Nur
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2014
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/41565/
http://irep.iium.edu.my/41565/
http://irep.iium.edu.my/41565/4/P_59.pdf
id iium-41565
recordtype eprints
spelling iium-415652018-01-04T08:48:01Z http://irep.iium.edu.my/41565/ Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans Yusof, Faridah Mohd Dom, Azratul Ashimah Nur TP248.13 Biotechnology Tetrathionate hydrolase produced by Thiobacillus ferrooxidans has been known to be the enzyme responsible in the devulcanization of used rubber prior to recycling. The aim of this study was to statistically obtain a model that would yield an optimized media concentrations for the best production of this bacterial enzyme. One-factor-at-a-time (OFAT) experimental design was applied to find the possible optimum range of five media components. Based on the OFAT results, a face centered central composite design (FCCCD) by Design Expert? 6.0.8 was applied using a 4-factors, 3-center points to find the optimum media concentrations to yield the highest tetrathionate hydrolase. Response Surface Methodology (RSM) predicted the level of factors for maximized response. In this study, it is concluded that the medium containing 0.4g/L MgSO4.7H2O, 4.0g/L (NH4)2SO4, 2.0g/L Na2S2O3.5H2O and 3.0 g/L KH2PO4 results in the production of the highest specific activity of tetrathionate hydrolase (295.7 U/mg Protein). Other parametric conditions used were 0.25g/L for CaCl2.2H2O with an initial pH buffer of 4, incubated at 25oC while agitating at a speed of 125 rpm. Keywords : Thiobacillusferrooxidans, tetrathionate hydrolase, OFAT, FCCCD, devulcanization of rubber. Journal of Pure and Applied Microbiology 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41565/4/P_59.pdf Yusof, Faridah and Mohd Dom, Azratul Ashimah Nur (2014) Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans. Journal of Pure and Applied Microbiology, 8 (6). pp. 4465-4469. ISSN 0973-7510 http://www.microbiologyjournal.org/index.php
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Yusof, Faridah
Mohd Dom, Azratul Ashimah Nur
Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans
description Tetrathionate hydrolase produced by Thiobacillus ferrooxidans has been known to be the enzyme responsible in the devulcanization of used rubber prior to recycling. The aim of this study was to statistically obtain a model that would yield an optimized media concentrations for the best production of this bacterial enzyme. One-factor-at-a-time (OFAT) experimental design was applied to find the possible optimum range of five media components. Based on the OFAT results, a face centered central composite design (FCCCD) by Design Expert? 6.0.8 was applied using a 4-factors, 3-center points to find the optimum media concentrations to yield the highest tetrathionate hydrolase. Response Surface Methodology (RSM) predicted the level of factors for maximized response. In this study, it is concluded that the medium containing 0.4g/L MgSO4.7H2O, 4.0g/L (NH4)2SO4, 2.0g/L Na2S2O3.5H2O and 3.0 g/L KH2PO4 results in the production of the highest specific activity of tetrathionate hydrolase (295.7 U/mg Protein). Other parametric conditions used were 0.25g/L for CaCl2.2H2O with an initial pH buffer of 4, incubated at 25oC while agitating at a speed of 125 rpm. Keywords : Thiobacillusferrooxidans, tetrathionate hydrolase, OFAT, FCCCD, devulcanization of rubber.
format Article
author Yusof, Faridah
Mohd Dom, Azratul Ashimah Nur
author_facet Yusof, Faridah
Mohd Dom, Azratul Ashimah Nur
author_sort Yusof, Faridah
title Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans
title_short Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans
title_full Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans
title_fullStr Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans
title_full_unstemmed Optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans
title_sort optimization of media components for tetrathionate hydrolase production from thiobacillus ferrooxidans
publisher Journal of Pure and Applied Microbiology
publishDate 2014
url http://irep.iium.edu.my/41565/
http://irep.iium.edu.my/41565/
http://irep.iium.edu.my/41565/4/P_59.pdf
first_indexed 2023-09-18T20:59:30Z
last_indexed 2023-09-18T20:59:30Z
_version_ 1777410528452280320

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