Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals

Diabetes mellitus is a chronic disorder together with other metabolic abnormalities, usually developed due to insulin resistance or deficiency as well as increased hepatic glucose output [1]. The prevalence of diabetes mellitus among Malaysians adults more than 30 years of age has escalated by more...

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Main Authors:	Ahmed, Qamar Uddin, Afrin, Mehnaz, Mat So'ad, Siti Zaiton, Wan Sulaiman, Wan Azizi, Bakhtiar, M. Taher, Latip, Jalifah, Saxena, Anil Kumar
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41543/ http://irep.iium.edu.my/41543/ http://irep.iium.edu.my/41543/2/isolation.pdf

id	iium-41543
recordtype	eprints
spelling	iium-415432018-06-11T05:59:05Z http://irep.iium.edu.my/41543/ Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals Ahmed, Qamar Uddin Afrin, Mehnaz Mat So'ad, Siti Zaiton Wan Sulaiman, Wan Azizi Bakhtiar, M. Taher Latip, Jalifah Saxena, Anil Kumar R Medicine (General) Diabetes mellitus is a chronic disorder together with other metabolic abnormalities, usually developed due to insulin resistance or deficiency as well as increased hepatic glucose output [1]. The prevalence of diabetes mellitus among Malaysians adults more than 30 years of age has escalated by more than two fold over a 20-year period [2]. On the other hand, many plants have been used for the treatment of diabetes mellitus in modern system of medicine and in other ancient systems of the world. As a result, plants are now-a-days regarded as promising and significantly attractive natural sources to enrich the current therapy options against diabetes. However, so far, not many active agents have been successfully isolated and extensively studied [3]. Hence, we prompted to evaluate the antidiabetic potential of a flavonoid (5,7-dihydroxy-8-methoxyflavone [4], Figure 1) isolated from the leaves of Tetracera indica Merr., a Malaysian medicinal plant which is traditionally used to treat diabetes in Malaysia. The study was carried out using Sprague-Dawley rats (diabetic as well as normal) to evaluate antidiabetic potential of 5,7-dihydroxy-8-methoxyflavone at three different concentrations, viz. 1 mg/kg b.w., 5 mg/kg b.w. and 25 mg/kg b.w. At 5 mg/kg b.w. and 25 mg/kg b.w., it was found to exhibit significant anti-hyperglycemic activity in alloxan induced diabetic rats and in normal rats, no hypoglycemic activity was observed at all concentrations, when compared with +ve and –ve controlled groups. The antidiabetic activity was found to be comparable with glibenclamide (GLBC), a known oral hypoglycemic agent (50 mg/kg b.w.) (Table 1). The LD50 of 5,7-dihydroxy-8-methoxyflavone was found to be more than 500 mg/kg b.w. and no lethal toxicity was observed within this range. Three weeks later, the in-vivo study, histopathology of kidney and pancreas from alloxan-induced rats demonstrated the clinical manifestation of diabetic affected kidney and pancreas (Figures 2 & 3). It is concluded that the 5,7-dihydroxy-8-methoxyflavone from the leaves of T. indica is a safe and promising antidiabetic agent that could prove useful in the management of diabetes and might also provide lead for the synthesis of a new class of safe antidiabetic drugs. Keywords: 5,7-dihydroxy-8-methoxyflavone, Tetracera indica, antidiabetic agent 2014-08-16 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/41543/2/isolation.pdf Ahmed, Qamar Uddin and Afrin, Mehnaz and Mat So'ad, Siti Zaiton and Wan Sulaiman, Wan Azizi and Bakhtiar, M. Taher and Latip, Jalifah and Saxena, Anil Kumar (2014) Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals. In: 1st International Conference on Industrial Pharmacy (ICIP) 2014, 16th-17th August 2014, Swiss Garden Resort & Spa, Kuantan, Pahang, Malaysia. (Unpublished) http://www.iium.edu.my/icip2014/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Ahmed, Qamar Uddin Afrin, Mehnaz Mat So'ad, Siti Zaiton Wan Sulaiman, Wan Azizi Bakhtiar, M. Taher Latip, Jalifah Saxena, Anil Kumar Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals
description	Diabetes mellitus is a chronic disorder together with other metabolic abnormalities, usually developed due to insulin resistance or deficiency as well as increased hepatic glucose output [1]. The prevalence of diabetes mellitus among Malaysians adults more than 30 years of age has escalated by more than two fold over a 20-year period [2]. On the other hand, many plants have been used for the treatment of diabetes mellitus in modern system of medicine and in other ancient systems of the world. As a result, plants are now-a-days regarded as promising and significantly attractive natural sources to enrich the current therapy options against diabetes. However, so far, not many active agents have been successfully isolated and extensively studied [3]. Hence, we prompted to evaluate the antidiabetic potential of a flavonoid (5,7-dihydroxy-8-methoxyflavone [4], Figure 1) isolated from the leaves of Tetracera indica Merr., a Malaysian medicinal plant which is traditionally used to treat diabetes in Malaysia. The study was carried out using Sprague-Dawley rats (diabetic as well as normal) to evaluate antidiabetic potential of 5,7-dihydroxy-8-methoxyflavone at three different concentrations, viz. 1 mg/kg b.w., 5 mg/kg b.w. and 25 mg/kg b.w. At 5 mg/kg b.w. and 25 mg/kg b.w., it was found to exhibit significant anti-hyperglycemic activity in alloxan induced diabetic rats and in normal rats, no hypoglycemic activity was observed at all concentrations, when compared with +ve and –ve controlled groups. The antidiabetic activity was found to be comparable with glibenclamide (GLBC), a known oral hypoglycemic agent (50 mg/kg b.w.) (Table 1). The LD50 of 5,7-dihydroxy-8-methoxyflavone was found to be more than 500 mg/kg b.w. and no lethal toxicity was observed within this range. Three weeks later, the in-vivo study, histopathology of kidney and pancreas from alloxan-induced rats demonstrated the clinical manifestation of diabetic affected kidney and pancreas (Figures 2 & 3). It is concluded that the 5,7-dihydroxy-8-methoxyflavone from the leaves of T. indica is a safe and promising antidiabetic agent that could prove useful in the management of diabetes and might also provide lead for the synthesis of a new class of safe antidiabetic drugs. Keywords: 5,7-dihydroxy-8-methoxyflavone, Tetracera indica, antidiabetic agent
format	Conference or Workshop Item
author	Ahmed, Qamar Uddin Afrin, Mehnaz Mat So'ad, Siti Zaiton Wan Sulaiman, Wan Azizi Bakhtiar, M. Taher Latip, Jalifah Saxena, Anil Kumar
author_facet	Ahmed, Qamar Uddin Afrin, Mehnaz Mat So'ad, Siti Zaiton Wan Sulaiman, Wan Azizi Bakhtiar, M. Taher Latip, Jalifah Saxena, Anil Kumar
author_sort	Ahmed, Qamar Uddin
title	Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals
title_short	Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals
title_full	Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals
title_fullStr	Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals
title_full_unstemmed	Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals
title_sort	isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals
publishDate	2014
url	http://irep.iium.edu.my/41543/ http://irep.iium.edu.my/41543/ http://irep.iium.edu.my/41543/2/isolation.pdf
first_indexed	2023-09-18T20:59:29Z
last_indexed	2023-09-18T20:59:29Z
_version_	1777410526967496704

Isolation of a promising antidiabetic compound from the leaves of tetracera indica merr., and in vivo toxicological studies in diabetes induced experimental animals

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