High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector

Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). It is a class of drug that provides analgesic and antipyretic (fever reducing effect) and in higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic ac...

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Main Authors:	Helaluddin , Abul Bashar Mohammed, Mohamad , Huda Jamilah, Alaama, Mohammed, Amiruddin , Noor Syafawati
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/41453/ http://irep.iium.edu.my/41453/1/IPharME-2014.pdf

id	iium-41453
recordtype	eprints
spelling	iium-414532015-04-23T04:34:13Z http://irep.iium.edu.my/41453/ High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector Helaluddin , Abul Bashar Mohammed Mohamad , Huda Jamilah Alaama, Mohammed Amiruddin , Noor Syafawati QD Chemistry RS Pharmacy and materia medica Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). It is a class of drug that provides analgesic and antipyretic (fever reducing effect) and in higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma. A simple and sensitive high-performance liquid chromatography-UV detection method was developed for the detection of mefenamic acid. Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 (150 mm x 4.6 mm, i.d 3.5µm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored under 280 nm using variable-wavelength detector. The recovery was found to be 83% for MA. The method was validated for following the CDER (Centre for Drug Evaluation and Research) guideline. Calibration plot were linear within the range from 250 to 5000ng ml-1 with the correlation coefficient (r2) of ≥ 0.99. The result from analysis of quality control of mefenamic acid which were termed as low, medium and high were analysed to get the precision and accuracy. The accuracy for intra-day for low, medium and high were 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient of variance (CV) for intra-day precision for low, medium and high were found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study. Keyword Mefenamic acid, HPLC, plasma, protein precipitation technique. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/41453/1/IPharME-2014.pdf Helaluddin , Abul Bashar Mohammed and Mohamad , Huda Jamilah and Alaama, Mohammed and Amiruddin , Noor Syafawati (2014) High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector. In: International Conference on Pharmaceutical, Medical & Environmental Health Sciences (IPharME, 2014), 17-18 October, 2014, Park Royal, Penang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Helaluddin , Abul Bashar Mohammed Mohamad , Huda Jamilah Alaama, Mohammed Amiruddin , Noor Syafawati High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector
description	Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). It is a class of drug that provides analgesic and antipyretic (fever reducing effect) and in higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma. A simple and sensitive high-performance liquid chromatography-UV detection method was developed for the detection of mefenamic acid. Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 (150 mm x 4.6 mm, i.d 3.5µm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored under 280 nm using variable-wavelength detector. The recovery was found to be 83% for MA. The method was validated for following the CDER (Centre for Drug Evaluation and Research) guideline. Calibration plot were linear within the range from 250 to 5000ng ml-1 with the correlation coefficient (r2) of ≥ 0.99. The result from analysis of quality control of mefenamic acid which were termed as low, medium and high were analysed to get the precision and accuracy. The accuracy for intra-day for low, medium and high were 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient of variance (CV) for intra-day precision for low, medium and high were found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study. Keyword Mefenamic acid, HPLC, plasma, protein precipitation technique.
format	Conference or Workshop Item
author	Helaluddin , Abul Bashar Mohammed Mohamad , Huda Jamilah Alaama, Mohammed Amiruddin , Noor Syafawati
author_facet	Helaluddin , Abul Bashar Mohammed Mohamad , Huda Jamilah Alaama, Mohammed Amiruddin , Noor Syafawati
author_sort	Helaluddin , Abul Bashar Mohammed
title	High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector
title_short	High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector
title_full	High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector
title_fullStr	High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector
title_full_unstemmed	High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector
title_sort	high performance liquid chromatographic determination of mefenamic acid in human plasma using uv vis detector
publishDate	2014
url	http://irep.iium.edu.my/41453/ http://irep.iium.edu.my/41453/1/IPharME-2014.pdf
first_indexed	2023-09-18T20:59:22Z
last_indexed	2023-09-18T20:59:22Z
_version_	1777410519870734336

High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector

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