Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties

The present study demonstrates the antioxidant activity and cell migration effect of a standardized extract of C. asiatica. The ethanol extract was fractionated into seven fractions via vacuum liquid chromatography. The interest compound in the fractions was qualitatively identified using thin layer...

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Main Authors:	Abdul Aziz, Huwaida, Bakhtiar, M. Taher, Sh. Ahmad, Afnan, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/41436/ http://irep.iium.edu.my/41436/ http://irep.iium.edu.my/41436/1/41436.pdf

id	iium-41436
recordtype	eprints
spelling	iium-414362016-05-23T03:11:54Z http://irep.iium.edu.my/41436/ Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties Abdul Aziz, Huwaida Bakhtiar, M. Taher Sh. Ahmad, Afnan Darnis, Deny Susanti RS Pharmacy and materia medica The present study demonstrates the antioxidant activity and cell migration effect of a standardized extract of C. asiatica. The ethanol extract was fractionated into seven fractions via vacuum liquid chromatography. The interest compound in the fractions was qualitatively identified using thin layer chromatography and the positive fraction with asiaticoside was further quantified using reverse-phase HPLC. The result showed that the methanol fraction of extract contains more than 10% of asiaticoside. The fraction also exhibited antioxidant activity with IC50 value of 463.8 µg/mL as DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenger while IC50 value of 587.9 µg/mL in ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging capacities.The asiaticoside rich fraction was tested on mouse embryonic fibroblast NIH3T3 and human dermal keratinocyte HaCaT. Based on cell viability test of MTT (methylthiazol tetrazolium) colorimetric assay, both mouse embryonic fibroblast NIH3T3 and human dermal keratinocyte HaCaT showed significant stimulation against methanolic fraction of extract at the concentration of 100 µg/mL and 0.19 µg/mL. Besides, the extract showed almost no toxicity effect at concentrations tested since their IC50 could not be determined in concentration ranging from 100 µg/mL to 0.19 µg/mL. Due to all the concentration tested caused more than 80% cell viability, the concentrations chosen for the scratch assay is randomly chosen based on their concentration level of highest (100 µg/mL), medium (6 µg/mL), and lowest (0.2 µg/mL). In the scratch assay, only methanol fraction of extract with concentration 0.2 µg/mL showed significant cell migration effect on HaCaT compared to positive control (P < 0.05). 2015-01 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/41436/1/41436.pdf Abdul Aziz, Huwaida and Bakhtiar, M. Taher and Sh. Ahmad, Afnan and Darnis, Deny Susanti (2015) Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties. In: International Conference on Biomedical & Health Sciences Research (ICBHSR 2015), 25-27 Jan. 2015, The Everly Hotel, Putrajaya. http://www.icbhsr.com
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Abdul Aziz, Huwaida Bakhtiar, M. Taher Sh. Ahmad, Afnan Darnis, Deny Susanti Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties
description	The present study demonstrates the antioxidant activity and cell migration effect of a standardized extract of C. asiatica. The ethanol extract was fractionated into seven fractions via vacuum liquid chromatography. The interest compound in the fractions was qualitatively identified using thin layer chromatography and the positive fraction with asiaticoside was further quantified using reverse-phase HPLC. The result showed that the methanol fraction of extract contains more than 10% of asiaticoside. The fraction also exhibited antioxidant activity with IC50 value of 463.8 µg/mL as DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenger while IC50 value of 587.9 µg/mL in ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging capacities.The asiaticoside rich fraction was tested on mouse embryonic fibroblast NIH3T3 and human dermal keratinocyte HaCaT. Based on cell viability test of MTT (methylthiazol tetrazolium) colorimetric assay, both mouse embryonic fibroblast NIH3T3 and human dermal keratinocyte HaCaT showed significant stimulation against methanolic fraction of extract at the concentration of 100 µg/mL and 0.19 µg/mL. Besides, the extract showed almost no toxicity effect at concentrations tested since their IC50 could not be determined in concentration ranging from 100 µg/mL to 0.19 µg/mL. Due to all the concentration tested caused more than 80% cell viability, the concentrations chosen for the scratch assay is randomly chosen based on their concentration level of highest (100 µg/mL), medium (6 µg/mL), and lowest (0.2 µg/mL). In the scratch assay, only methanol fraction of extract with concentration 0.2 µg/mL showed significant cell migration effect on HaCaT compared to positive control (P < 0.05).
format	Conference or Workshop Item
author	Abdul Aziz, Huwaida Bakhtiar, M. Taher Sh. Ahmad, Afnan Darnis, Deny Susanti
author_facet	Abdul Aziz, Huwaida Bakhtiar, M. Taher Sh. Ahmad, Afnan Darnis, Deny Susanti
author_sort	Abdul Aziz, Huwaida
title	Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties
title_short	Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties
title_full	Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties
title_fullStr	Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties
title_full_unstemmed	Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties
title_sort	antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties
publishDate	2015
url	http://irep.iium.edu.my/41436/ http://irep.iium.edu.my/41436/ http://irep.iium.edu.my/41436/1/41436.pdf
first_indexed	2023-09-18T20:59:21Z
last_indexed	2023-09-18T20:59:21Z
_version_	1777410518851518464

Antioxidant activity and cell migration effect of standardized extract of centella asiatica for wound healing properties

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