Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering

Articular cartilage tissue is known to have poor capability of self-repair. Therefore, tissue engineering has significantly emerged as an alternative solution for articular cartilage restoration. Poly(lactic-co-glycolic acid) (PLGA) and fibrin have been widely used as biocompatible scaffolds materia...

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Main Authors:	Abdul Rahman, Rozlin, A. M., Noradibah, Azeman, Nor Athirah, Mohamad Sukri, Norhamiza, Md Nazir, Norhidayah, Ahmad Radzi, Muhammad Aa'zamuddin, Mohamad, Mohd Yusof, Zulkifly, Ahmad Hafiz, Che Ahmad, Aminuddin, Abdul Rahman, Suzanah, Sha'ban, Munirah
Format:	Article
Language:	English
Published:	Tissue Engineering and Regenerative Medicine Society of Malaysia 2014
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41428/ http://irep.iium.edu.my/41428/ http://irep.iium.edu.my/41428/1/RR-181114-090.pdf

id	iium-41428
recordtype	eprints
spelling	iium-414282018-06-29T03:04:04Z http://irep.iium.edu.my/41428/ Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering Abdul Rahman, Rozlin A. M., Noradibah Azeman, Nor Athirah Mohamad Sukri, Norhamiza Md Nazir, Norhidayah Ahmad Radzi, Muhammad Aa'zamuddin Mohamad, Mohd Yusof Zulkifly, Ahmad Hafiz Che Ahmad, Aminuddin Abdul Rahman, Suzanah Sha'ban, Munirah R Medicine (General) Articular cartilage tissue is known to have poor capability of self-repair. Therefore, tissue engineering has significantly emerged as an alternative solution for articular cartilage restoration. Poly(lactic-co-glycolic acid) (PLGA) and fibrin have been widely used as biocompatible scaffolds materials to regenerate tissue. In this study, bone marrow mesenchymal stem cells (BMMSCs) were seeded onto the PLGA with (PLGA/Fibrin) or without fibrin (PLGA only). Macroscopic observation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and sulphated glycosaminoglycan (sGAG) assays were performed at week 1, 2 and 3 in vitro. Both PLGA/Fibrin and PLGA only groups showed similar hyaline-like appearance with white and smooth glistening surface. The MTT assay showed higher cell proliferation in the PLGA/Fibrin than the PLGA only group. The production of sGAG is higher in the PLGA/Fibrin group than in the PLGA only. This may suggest that PLGA/Fibrin may serve as prospective cell delivery vehicle and essential foundation for in vitro tissue engineered articular cartilage. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41428/1/RR-181114-090.pdf Abdul Rahman, Rozlin and A. M., Noradibah and Azeman, Nor Athirah and Mohamad Sukri, Norhamiza and Md Nazir, Norhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Mohamad, Mohd Yusof and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminuddin and Abdul Rahman, Suzanah and Sha'ban, Munirah (2014) Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering. Regenerative Research, 3 (2). pp. 163-164. ISSN 2232-0822 (Online) http://regres.tesma.org.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abdul Rahman, Rozlin A. M., Noradibah Azeman, Nor Athirah Mohamad Sukri, Norhamiza Md Nazir, Norhidayah Ahmad Radzi, Muhammad Aa'zamuddin Mohamad, Mohd Yusof Zulkifly, Ahmad Hafiz Che Ahmad, Aminuddin Abdul Rahman, Suzanah Sha'ban, Munirah Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering
description	Articular cartilage tissue is known to have poor capability of self-repair. Therefore, tissue engineering has significantly emerged as an alternative solution for articular cartilage restoration. Poly(lactic-co-glycolic acid) (PLGA) and fibrin have been widely used as biocompatible scaffolds materials to regenerate tissue. In this study, bone marrow mesenchymal stem cells (BMMSCs) were seeded onto the PLGA with (PLGA/Fibrin) or without fibrin (PLGA only). Macroscopic observation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and sulphated glycosaminoglycan (sGAG) assays were performed at week 1, 2 and 3 in vitro. Both PLGA/Fibrin and PLGA only groups showed similar hyaline-like appearance with white and smooth glistening surface. The MTT assay showed higher cell proliferation in the PLGA/Fibrin than the PLGA only group. The production of sGAG is higher in the PLGA/Fibrin group than in the PLGA only. This may suggest that PLGA/Fibrin may serve as prospective cell delivery vehicle and essential foundation for in vitro tissue engineered articular cartilage.
format	Article
author	Abdul Rahman, Rozlin A. M., Noradibah Azeman, Nor Athirah Mohamad Sukri, Norhamiza Md Nazir, Norhidayah Ahmad Radzi, Muhammad Aa'zamuddin Mohamad, Mohd Yusof Zulkifly, Ahmad Hafiz Che Ahmad, Aminuddin Abdul Rahman, Suzanah Sha'ban, Munirah
author_facet	Abdul Rahman, Rozlin A. M., Noradibah Azeman, Nor Athirah Mohamad Sukri, Norhamiza Md Nazir, Norhidayah Ahmad Radzi, Muhammad Aa'zamuddin Mohamad, Mohd Yusof Zulkifly, Ahmad Hafiz Che Ahmad, Aminuddin Abdul Rahman, Suzanah Sha'ban, Munirah
author_sort	Abdul Rahman, Rozlin
title	Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering
title_short	Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering
title_full	Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering
title_fullStr	Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering
title_full_unstemmed	Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering
title_sort	cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering
publisher	Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate	2014
url	http://irep.iium.edu.my/41428/ http://irep.iium.edu.my/41428/ http://irep.iium.edu.my/41428/1/RR-181114-090.pdf
first_indexed	2023-09-18T20:59:20Z
last_indexed	2023-09-18T20:59:20Z
_version_	1777410518267461632

Cell proliferation assay and sulphated glycosaminoglycan production in poly(lactic-co-glycolic acid)-based scaffolds seeded with bone marrow mesenchymal stem cells for cartilage tissue engineering

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