Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface

The study aims to evaluate the osteogenic potential of human adipose derived stem cell (HADSC) co-culture with human osteoblast (HOB) using selected HADSC/HOB ratio of 2:1, 1:1 and 1:2, respectively. The HADSC/HOB was seeded on Titanium dioxide (TiO2) coated with or without nanofibre substrate. The...

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Main Authors:	Ismail, Rozila, Tan, A. W., Pingguan-Murphy, Belinda, Sha'ban, Munirah, Chua, Kien Hui
Format:	Article
Language:	English
Published:	Tissue Engineering and Regenerative Medicine Society of Malaysia 2014
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41424/ http://irep.iium.edu.my/41424/ http://irep.iium.edu.my/41424/1/RR-181114-041.pdf

id	iium-41424
recordtype	eprints
spelling	iium-414242015-02-12T09:11:39Z http://irep.iium.edu.my/41424/ Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface Ismail, Rozila Tan, A. W. Pingguan-Murphy, Belinda Sha'ban, Munirah Chua, Kien Hui R Medicine (General) The study aims to evaluate the osteogenic potential of human adipose derived stem cell (HADSC) co-culture with human osteoblast (HOB) using selected HADSC/HOB ratio of 2:1, 1:1 and 1:2, respectively. The HADSC/HOB was seeded on Titanium dioxide (TiO2) coated with or without nanofibre substrate. The non-coated TiO2 was used as control. The effects of TiO2 based scaffolds on cell adhesion were characterized by scanning electron microscopy (SEM). Cell viability, differentiation and mineralization were assessed by Alamar Blue, alkaline phosphatase (ALP) and Alizarin Red assays, respectively. The combination of HADSC/HOB, 2:1 ratio, seeded on nanofibrous-coated TiO2 showed better cell adhesion, viability, differentiation and mineralization than the other groups. This study offers opportunity to assess in vitro cellular development of HADSC through direct cell to cell contact with HOB. This study indicates that the co-cultured HADSC/HOB seeded on TiO2 based scaffolds may serve as a promising approach to facilitate osteogenic differentiation activity. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41424/1/RR-181114-041.pdf Ismail, Rozila and Tan, A. W. and Pingguan-Murphy, Belinda and Sha'ban, Munirah and Chua, Kien Hui (2014) Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface. Regenerative Research, 3 (2). pp. 65-66. ISSN 2232-0822 (Online) http://regres.tesma.org.my/pdf/RR-181114-041.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Ismail, Rozila Tan, A. W. Pingguan-Murphy, Belinda Sha'ban, Munirah Chua, Kien Hui Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
description	The study aims to evaluate the osteogenic potential of human adipose derived stem cell (HADSC) co-culture with human osteoblast (HOB) using selected HADSC/HOB ratio of 2:1, 1:1 and 1:2, respectively. The HADSC/HOB was seeded on Titanium dioxide (TiO2) coated with or without nanofibre substrate. The non-coated TiO2 was used as control. The effects of TiO2 based scaffolds on cell adhesion were characterized by scanning electron microscopy (SEM). Cell viability, differentiation and mineralization were assessed by Alamar Blue, alkaline phosphatase (ALP) and Alizarin Red assays, respectively. The combination of HADSC/HOB, 2:1 ratio, seeded on nanofibrous-coated TiO2 showed better cell adhesion, viability, differentiation and mineralization than the other groups. This study offers opportunity to assess in vitro cellular development of HADSC through direct cell to cell contact with HOB. This study indicates that the co-cultured HADSC/HOB seeded on TiO2 based scaffolds may serve as a promising approach to facilitate osteogenic differentiation activity.
format	Article
author	Ismail, Rozila Tan, A. W. Pingguan-Murphy, Belinda Sha'ban, Munirah Chua, Kien Hui
author_facet	Ismail, Rozila Tan, A. W. Pingguan-Murphy, Belinda Sha'ban, Munirah Chua, Kien Hui
author_sort	Ismail, Rozila
title	Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_short	Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_full	Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_fullStr	Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_full_unstemmed	Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_sort	osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
publisher	Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate	2014
url	http://irep.iium.edu.my/41424/ http://irep.iium.edu.my/41424/ http://irep.iium.edu.my/41424/1/RR-181114-041.pdf
first_indexed	2023-09-18T20:59:20Z
last_indexed	2023-09-18T20:59:20Z
_version_	1777410517673967616

Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface

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