Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds

Tissue engineering and regenerative medicine has the potential of improving the quality of life for many people. One particular area that presents an exciting challenge is the regeneration of intervertebral discs (IVD). To achieve this, it is necessary to identify suitable cells, biomaterial scaffol...

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Main Authors:	Md Nazir, Noorhidayah, T. M., Tengku Nuramirah, Razak, Syahidah, Mohamad, Mohd Yusof, Abdul Rahman, Rozlin, Mohamad Syukri, Norhamiza, Ahmad Radzi, Muhammad Aa'zamuddin, Zulkifly, Ahmad Hafiz, Sha'ban, Munirah
Format:	Article
Language:	English
Published:	Tissue Engineering and Regenerative Medicine Society of Malaysia 2014
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41420/ http://irep.iium.edu.my/41420/ http://irep.iium.edu.my/41420/3/RR-181114-032.pdf

id	iium-41420
recordtype	eprints
spelling	iium-414202015-02-12T09:07:29Z http://irep.iium.edu.my/41420/ Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds Md Nazir, Noorhidayah T. M., Tengku Nuramirah Razak, Syahidah Mohamad, Mohd Yusof Abdul Rahman, Rozlin Mohamad Syukri, Norhamiza Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Sha'ban, Munirah R Medicine (General) Tissue engineering and regenerative medicine has the potential of improving the quality of life for many people. One particular area that presents an exciting challenge is the regeneration of intervertebral discs (IVD). To achieve this, it is necessary to identify suitable cells, biomaterial scaffolds and biologically active signalling factors for in vitro and in vivo IVD generation. This present study aimed to evaluate the cartilaginous properties of nucleus pulposus (NP) cells seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin scaffolds by measuring sulphated glycosaminoglycan (sGAG) content and collagen type II (COL-II) gene expression. The analyses were performed at week 1, 2, and 3 in vitro. The sGAG content was higher in the PLGA/Fibrin than in the PLGA only group. The expression of COL-II was only detected at week-2 culture in PLGA/Fibrin group. This study indicates that PLGA-based scaffolds may have the potential for intervertebral discs tissue engineering. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41420/3/RR-181114-032.pdf Md Nazir, Noorhidayah and T. M., Tengku Nuramirah and Razak, Syahidah and Mohamad, Mohd Yusof and Abdul Rahman, Rozlin and Mohamad Syukri, Norhamiza and Ahmad Radzi, Muhammad Aa'zamuddin and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2014) Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds. Regenerative Research, 3 (2). pp. 46-47. ISSN 2232-0822 (Online) http://regres.tesma.org.my/pdf/RR-181114-032.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Md Nazir, Noorhidayah T. M., Tengku Nuramirah Razak, Syahidah Mohamad, Mohd Yusof Abdul Rahman, Rozlin Mohamad Syukri, Norhamiza Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Sha'ban, Munirah Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds
description	Tissue engineering and regenerative medicine has the potential of improving the quality of life for many people. One particular area that presents an exciting challenge is the regeneration of intervertebral discs (IVD). To achieve this, it is necessary to identify suitable cells, biomaterial scaffolds and biologically active signalling factors for in vitro and in vivo IVD generation. This present study aimed to evaluate the cartilaginous properties of nucleus pulposus (NP) cells seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin scaffolds by measuring sulphated glycosaminoglycan (sGAG) content and collagen type II (COL-II) gene expression. The analyses were performed at week 1, 2, and 3 in vitro. The sGAG content was higher in the PLGA/Fibrin than in the PLGA only group. The expression of COL-II was only detected at week-2 culture in PLGA/Fibrin group. This study indicates that PLGA-based scaffolds may have the potential for intervertebral discs tissue engineering.
format	Article
author	Md Nazir, Noorhidayah T. M., Tengku Nuramirah Razak, Syahidah Mohamad, Mohd Yusof Abdul Rahman, Rozlin Mohamad Syukri, Norhamiza Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Sha'ban, Munirah
author_facet	Md Nazir, Noorhidayah T. M., Tengku Nuramirah Razak, Syahidah Mohamad, Mohd Yusof Abdul Rahman, Rozlin Mohamad Syukri, Norhamiza Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Sha'ban, Munirah
author_sort	Md Nazir, Noorhidayah
title	Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds
title_short	Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds
title_full	Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds
title_fullStr	Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds
title_full_unstemmed	Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds
title_sort	cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds
publisher	Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate	2014
url	http://irep.iium.edu.my/41420/ http://irep.iium.edu.my/41420/ http://irep.iium.edu.my/41420/3/RR-181114-032.pdf
first_indexed	2023-09-18T20:59:20Z
last_indexed	2023-09-18T20:59:20Z
_version_	1777410517520875520

Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds

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