Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry

Traditional/medicine (TM) has a significant contribution to the global health care system. During the past few decades the use of TM has progressively increased worldwide. Therefore, safety and quality of such products become a major concern. Some herbal medicines may contain toxic substances such a...

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Main Authors:	Helaluddin , Abul Bashar Mohammed, Khalid , Reem S., Khan, Umeed Ali, Abbas, Syed Atif
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/41417/ http://irep.iium.edu.my/41417/1/ICNP-1.pdf

id	iium-41417
recordtype	eprints
spelling	iium-414172015-04-22T09:06:00Z http://irep.iium.edu.my/41417/ Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry Helaluddin , Abul Bashar Mohammed Khalid , Reem S. Khan, Umeed Ali Abbas, Syed Atif QD Chemistry RS Pharmacy and materia medica Traditional/medicine (TM) has a significant contribution to the global health care system. During the past few decades the use of TM has progressively increased worldwide. Therefore, safety and quality of such products become a major concern. Some herbal medicines may contain toxic substances such as heavy metals. Arsenic (As), cadmium (Cd), lead (Pb) and nickel (Ni) are among the toxic heavy metals which may be present in some herbal products. Exposure to these metals can cause many adverse health effects, including cancer. Zinc (Zn) and iron (Fe) are essential metals to the human body at trace concentrations yet, they are toxic if present in higher concentration. Heavy metals content in TM products must accurately determine. Sample preparation is a critical step in spectroscopic analysis to achieve reliable and accurate results. Wet digestion methods are basically used for the dissolution of herbal products samples prior to elemental analysis. Therefore, this study has been designed to evaluate the efficiency of three acid digestion methods using different solvents individual and combination of classically used acids for wet digestion procedure to identify the optimal sample preparation method which was evaluated based on the highest analyte recovery. Five TM samples of herbal origin were selected to be digested with three different acid digestion methods namely A, B and C which represented a combination of nitric-perchloric acids HNO3 – HClO4, nitric acid HNO3 and a mixture of hydrochloric – nitric acids HNO3 – HCl respectively. The analysis of heavy metals was conducted using various techniques of atomic absorption spectrometer (AAS). Hydride generation (HGAAS) for As, graphite furnace (GFAAS) for Cd, Pb and Ni while flame (FAAS) was used for the analysis of Zn and Fe. The statistical analysis revealed that Method -C was the most efficient as it had given a significant high recovery (p<0.05) for of As, Cd, Pb, Ni, Zn, and Fe in all samples compared to Method -A and Method- B. Method- C was validated with analysis of Standard Reference Material 1515 (SRM) obtained from NIST and recovery percentage for As, Cd, Pb, Ni, Zn and Fe were 102, 96, 106, 94.5, 108 and 100 respectively. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/41417/1/ICNP-1.pdf Helaluddin , Abul Bashar Mohammed and Khalid , Reem S. and Khan, Umeed Ali and Abbas, Syed Atif (2014) Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry. In: International Conference on Natural Products 2014, 18th-19th March 2014, Palm Garden Hotel, IOI Resorts, Putrajaya, Malaysia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Helaluddin , Abul Bashar Mohammed Khalid , Reem S. Khan, Umeed Ali Abbas, Syed Atif Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry
description	Traditional/medicine (TM) has a significant contribution to the global health care system. During the past few decades the use of TM has progressively increased worldwide. Therefore, safety and quality of such products become a major concern. Some herbal medicines may contain toxic substances such as heavy metals. Arsenic (As), cadmium (Cd), lead (Pb) and nickel (Ni) are among the toxic heavy metals which may be present in some herbal products. Exposure to these metals can cause many adverse health effects, including cancer. Zinc (Zn) and iron (Fe) are essential metals to the human body at trace concentrations yet, they are toxic if present in higher concentration. Heavy metals content in TM products must accurately determine. Sample preparation is a critical step in spectroscopic analysis to achieve reliable and accurate results. Wet digestion methods are basically used for the dissolution of herbal products samples prior to elemental analysis. Therefore, this study has been designed to evaluate the efficiency of three acid digestion methods using different solvents individual and combination of classically used acids for wet digestion procedure to identify the optimal sample preparation method which was evaluated based on the highest analyte recovery. Five TM samples of herbal origin were selected to be digested with three different acid digestion methods namely A, B and C which represented a combination of nitric-perchloric acids HNO3 – HClO4, nitric acid HNO3 and a mixture of hydrochloric – nitric acids HNO3 – HCl respectively. The analysis of heavy metals was conducted using various techniques of atomic absorption spectrometer (AAS). Hydride generation (HGAAS) for As, graphite furnace (GFAAS) for Cd, Pb and Ni while flame (FAAS) was used for the analysis of Zn and Fe. The statistical analysis revealed that Method -C was the most efficient as it had given a significant high recovery (p<0.05) for of As, Cd, Pb, Ni, Zn, and Fe in all samples compared to Method -A and Method- B. Method- C was validated with analysis of Standard Reference Material 1515 (SRM) obtained from NIST and recovery percentage for As, Cd, Pb, Ni, Zn and Fe were 102, 96, 106, 94.5, 108 and 100 respectively.
format	Conference or Workshop Item
author	Helaluddin , Abul Bashar Mohammed Khalid , Reem S. Khan, Umeed Ali Abbas, Syed Atif
author_facet	Helaluddin , Abul Bashar Mohammed Khalid , Reem S. Khan, Umeed Ali Abbas, Syed Atif
author_sort	Helaluddin , Abul Bashar Mohammed
title	Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry
title_short	Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry
title_full	Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry
title_fullStr	Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry
title_full_unstemmed	Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry
title_sort	optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry
publishDate	2014
url	http://irep.iium.edu.my/41417/ http://irep.iium.edu.my/41417/1/ICNP-1.pdf
first_indexed	2023-09-18T20:59:19Z
last_indexed	2023-09-18T20:59:19Z
_version_	1777410517074182144

Optimization of acid digestion methods for the determination of heavy metals in traditional medicine samples by atomic absorption spectrometry

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