Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments

Leech Saliva Extract (LSE) has been known to possess wide range of biological activities such as antithrombin, antidiabitic and anticancer. The natural bioactive peptides and proteins found in LSE are promising active pharmaceutical ingredient to develop new therapeutic pharmaceutical formulations....

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Main Authors:	Helaluddin , Abul Bashar Mohammed, Alaama, Mohammed, Abdualkader, Abdualrahman Mohammed, Khalid , Reem S.
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/41385/ http://irep.iium.edu.my/41385/ http://irep.iium.edu.my/41385/1/Bio_monitoring_Metals.pdf

id	iium-41385
recordtype	eprints
spelling	iium-413852015-04-22T04:19:23Z http://irep.iium.edu.my/41385/ Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Abdualkader, Abdualrahman Mohammed Khalid , Reem S. QD Chemistry RS Pharmacy and materia medica Leech Saliva Extract (LSE) has been known to possess wide range of biological activities such as antithrombin, antidiabitic and anticancer. The natural bioactive peptides and proteins found in LSE are promising active pharmaceutical ingredient to develop new therapeutic pharmaceutical formulations. Those formulations can provide benefits for health and environmental safety by applying green production procedure. The traces of heavy metals were investigated in the tissues and saliva extracts of leeches (Hirudinaria manillensis) collected from Cheneh lake, Terengganu, Malaysia. Concentration of selected heavy metals such as Cadmium (Cd), Chromium (Cr), Iron (Fe), Manganese (Mn), Nickel (Ni), Lead (Pb), Selenium (Se) and Zinc (Zn) were detected using ICP-MS. Almost all heavy metal concentrations in lake water were found to be higher than the Maximum Permissible Limits (MPL) and placed under class III Interim National Water Quality Standards For Malaysia (INWQS). Furthermore, trace amounts of heavy metals were found in leeches’ tissues and LSE. Such concentrations of heavy metals can be a source of health hazard if LSE product was prepared directly from the collected leeches without previous purification procedure. In this study, we described a method to reduce the metal levels in leech saliva by adopting frequent washing procedure using non-chlorinated or distilled water for a period of three successive weeks. The described procedure significantly reduced the concentrations of tested metals (p < 0.05), especially the deduction of Cd level up to 99.7% in leech saliva and 92.38% reduction in leeches’ tissues in case of Pb concentration. We conclude that constant washing procedure would help in safe utilization of LSE in green industrial pharmacy. Key words: leeches, saliva, extract, heavy metals, ICP-MS 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/41385/1/Bio_monitoring_Metals.pdf Helaluddin , Abul Bashar Mohammed and Alaama, Mohammed and Abdualkader, Abdualrahman Mohammed and Khalid , Reem S. (2014) Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments. In: 1st International Conference on Industrial Pharmacy (ICIP) 2014, 16th-17th August 2014, Swiss Garden Resort & Spa, Kuantan, Pahang, Malaysia. http://www.iium.edu.my/icip2014/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Abdualkader, Abdualrahman Mohammed Khalid , Reem S. Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments
description	Leech Saliva Extract (LSE) has been known to possess wide range of biological activities such as antithrombin, antidiabitic and anticancer. The natural bioactive peptides and proteins found in LSE are promising active pharmaceutical ingredient to develop new therapeutic pharmaceutical formulations. Those formulations can provide benefits for health and environmental safety by applying green production procedure. The traces of heavy metals were investigated in the tissues and saliva extracts of leeches (Hirudinaria manillensis) collected from Cheneh lake, Terengganu, Malaysia. Concentration of selected heavy metals such as Cadmium (Cd), Chromium (Cr), Iron (Fe), Manganese (Mn), Nickel (Ni), Lead (Pb), Selenium (Se) and Zinc (Zn) were detected using ICP-MS. Almost all heavy metal concentrations in lake water were found to be higher than the Maximum Permissible Limits (MPL) and placed under class III Interim National Water Quality Standards For Malaysia (INWQS). Furthermore, trace amounts of heavy metals were found in leeches’ tissues and LSE. Such concentrations of heavy metals can be a source of health hazard if LSE product was prepared directly from the collected leeches without previous purification procedure. In this study, we described a method to reduce the metal levels in leech saliva by adopting frequent washing procedure using non-chlorinated or distilled water for a period of three successive weeks. The described procedure significantly reduced the concentrations of tested metals (p < 0.05), especially the deduction of Cd level up to 99.7% in leech saliva and 92.38% reduction in leeches’ tissues in case of Pb concentration. We conclude that constant washing procedure would help in safe utilization of LSE in green industrial pharmacy. Key words: leeches, saliva, extract, heavy metals, ICP-MS
format	Conference or Workshop Item
author	Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Abdualkader, Abdualrahman Mohammed Khalid , Reem S.
author_facet	Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Abdualkader, Abdualrahman Mohammed Khalid , Reem S.
author_sort	Helaluddin , Abul Bashar Mohammed
title	Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments
title_short	Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments
title_full	Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments
title_fullStr	Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments
title_full_unstemmed	Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments
title_sort	biomonitoring metals in leeches (hirudinaria manillensis): a step towards its immediate application in traditional medical treatments
publishDate	2014
url	http://irep.iium.edu.my/41385/ http://irep.iium.edu.my/41385/ http://irep.iium.edu.my/41385/1/Bio_monitoring_Metals.pdf
first_indexed	2023-09-18T20:59:16Z
last_indexed	2023-09-18T20:59:16Z
_version_	1777410513973542912

Biomonitoring metals in leeches (Hirudinaria manillensis): a step towards its immediate application in traditional medical treatments

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