Comparison of alcohol free GSH production by ultrasonic and homogenizing method

Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the methods and the pH of the medium on the product...

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Main Authors:	Mohamed Salleh, Mohamad Ramlan, Jamal, Parveen, Gad, Abd. Elaziem Farouk, Hashim, Yumi Zuhanis Has-Yun, Salleh, Rohani, Hasim, Haslinda
Format:	Article
Language:	English
Published:	Iranian Journal of Chemistry and Chemical Engineering 2008
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/4108/ http://irep.iium.edu.my/4108/ http://irep.iium.edu.my/4108/1/200827414.pdf

id	iium-4108
recordtype	eprints
spelling	iium-41082013-07-19T03:00:48Z http://irep.iium.edu.my/4108/ Comparison of alcohol free GSH production by ultrasonic and homogenizing method Mohamed Salleh, Mohamad Ramlan Jamal, Parveen Gad, Abd. Elaziem Farouk Hashim, Yumi Zuhanis Has-Yun Salleh, Rohani Hasim, Haslinda TP248.13 Biotechnology Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the methods and the pH of the medium on the production of the GSH. The cell disruptions were run at a wide range of those parameters at the suitable common parameters of the methods. The disrupted cells suspension was then centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the absorbance (A) value of the solution by spectrophotometer at 412 nm. The results obtained showed that the production of GSH was affected by the adjusted specific parameters of each method and the yield of production by the homogenizing and ultrasonic method was found 3.3 and 4.1 times, respectively, better than the yield of production by the unadjusted parameters. Ultrasonic method which produced 23.2 mg/L product was found 1.3 times better than the homogenizing method (18.5 mg/L) in producing the GSH. The result obtained from the lab scale study of the above mentioned mechanical methods showed that the implementation of them in the large scale production of the alcohol free GSH is possible. Iranian Journal of Chemistry and Chemical Engineering 2008-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/4108/1/200827414.pdf Mohamed Salleh, Mohamad Ramlan and Jamal, Parveen and Gad, Abd. Elaziem Farouk and Hashim, Yumi Zuhanis Has-Yun and Salleh, Rohani and Hasim, Haslinda (2008) Comparison of alcohol free GSH production by ultrasonic and homogenizing method. Iranian Journal of Chemistry & Chemical Engineering, 27 (4). pp. 107-113. ISSN 1021-9986 http://www.ijcce.ac.ir/IJCCE/DetailArticleP.asp?Volume=27&Number=4&Ordered=14
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohamed Salleh, Mohamad Ramlan Jamal, Parveen Gad, Abd. Elaziem Farouk Hashim, Yumi Zuhanis Has-Yun Salleh, Rohani Hasim, Haslinda Comparison of alcohol free GSH production by ultrasonic and homogenizing method
description	Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the methods and the pH of the medium on the production of the GSH. The cell disruptions were run at a wide range of those parameters at the suitable common parameters of the methods. The disrupted cells suspension was then centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the absorbance (A) value of the solution by spectrophotometer at 412 nm. The results obtained showed that the production of GSH was affected by the adjusted specific parameters of each method and the yield of production by the homogenizing and ultrasonic method was found 3.3 and 4.1 times, respectively, better than the yield of production by the unadjusted parameters. Ultrasonic method which produced 23.2 mg/L product was found 1.3 times better than the homogenizing method (18.5 mg/L) in producing the GSH. The result obtained from the lab scale study of the above mentioned mechanical methods showed that the implementation of them in the large scale production of the alcohol free GSH is possible.
format	Article
author	Mohamed Salleh, Mohamad Ramlan Jamal, Parveen Gad, Abd. Elaziem Farouk Hashim, Yumi Zuhanis Has-Yun Salleh, Rohani Hasim, Haslinda
author_facet	Mohamed Salleh, Mohamad Ramlan Jamal, Parveen Gad, Abd. Elaziem Farouk Hashim, Yumi Zuhanis Has-Yun Salleh, Rohani Hasim, Haslinda
author_sort	Mohamed Salleh, Mohamad Ramlan
title	Comparison of alcohol free GSH production by ultrasonic and homogenizing method
title_short	Comparison of alcohol free GSH production by ultrasonic and homogenizing method
title_full	Comparison of alcohol free GSH production by ultrasonic and homogenizing method
title_fullStr	Comparison of alcohol free GSH production by ultrasonic and homogenizing method
title_full_unstemmed	Comparison of alcohol free GSH production by ultrasonic and homogenizing method
title_sort	comparison of alcohol free gsh production by ultrasonic and homogenizing method
publisher	Iranian Journal of Chemistry and Chemical Engineering
publishDate	2008
url	http://irep.iium.edu.my/4108/ http://irep.iium.edu.my/4108/ http://irep.iium.edu.my/4108/1/200827414.pdf
first_indexed	2023-09-18T20:12:12Z
last_indexed	2023-09-18T20:12:12Z
_version_	1777407552615612416

Comparison of alcohol free GSH production by ultrasonic and homogenizing method

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