Thermostable lipases: an overview of production, purification and characterization

Thermostable lipases occupy a prominent position in aqueous and non-aqueous biocatalysis. Isolation of wild strains with novel properties has been on-going and recombinant strains are being constructed so as to meet the biotechnological applications of lipases. Different purification methods prove...

Full description

Bibliographic Details
Main Authors: Salihu, Aliyu, Alam, Md Zahangir
Format: Article
Language:English
English
Published: Oriental Scientific Publishing Company 2014
Subjects:
Online Access:http://irep.iium.edu.my/40933/
http://irep.iium.edu.my/40933/
http://irep.iium.edu.my/40933/
http://irep.iium.edu.my/40933/1/Paper-aliyu.pdf
http://irep.iium.edu.my/40933/4/40933-Thermostable%20lipases_SCOPUS.pdf
id iium-40933
recordtype eprints
spelling iium-409332017-08-10T07:49:02Z http://irep.iium.edu.my/40933/ Thermostable lipases: an overview of production, purification and characterization Salihu, Aliyu Alam, Md Zahangir TP248.13 Biotechnology Thermostable lipases occupy a prominent position in aqueous and non-aqueous biocatalysis. Isolation of wild strains with novel properties has been on-going and recombinant strains are being constructed so as to meet the biotechnological applications of lipases. Different purification methods prove effective and the purity of the enzyme is dependent on the intended applications. Unlike medical and pharmaceutical applications where highly pure preparations are needed, partial purification can be sufficient for general applications. Thus, characterization associated with enzyme activity, specificity, thermostability, enatioselectivity and tolerance to various solvent systems are the prerequisite for thermostable lipase selection which make them highly demanding in lipase catalyzed reactions. This article intends to give an insight on thermostable lipases so as to stimulate researchers to explore other unique properties from wild and recombinant strains that could be of benefit for wider industrial applications. Oriental Scientific Publishing Company 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/40933/1/Paper-aliyu.pdf application/pdf en http://irep.iium.edu.my/40933/4/40933-Thermostable%20lipases_SCOPUS.pdf Salihu, Aliyu and Alam, Md Zahangir (2014) Thermostable lipases: an overview of production, purification and characterization. Biosciences Biotechnology Research Asia, 11 (3). pp. 1095-1107. ISSN 0973-1245 http://www.biotech-asia.org/index.php 10.13005/bbra/1494
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Salihu, Aliyu
Alam, Md Zahangir
Thermostable lipases: an overview of production, purification and characterization
description Thermostable lipases occupy a prominent position in aqueous and non-aqueous biocatalysis. Isolation of wild strains with novel properties has been on-going and recombinant strains are being constructed so as to meet the biotechnological applications of lipases. Different purification methods prove effective and the purity of the enzyme is dependent on the intended applications. Unlike medical and pharmaceutical applications where highly pure preparations are needed, partial purification can be sufficient for general applications. Thus, characterization associated with enzyme activity, specificity, thermostability, enatioselectivity and tolerance to various solvent systems are the prerequisite for thermostable lipase selection which make them highly demanding in lipase catalyzed reactions. This article intends to give an insight on thermostable lipases so as to stimulate researchers to explore other unique properties from wild and recombinant strains that could be of benefit for wider industrial applications.
format Article
author Salihu, Aliyu
Alam, Md Zahangir
author_facet Salihu, Aliyu
Alam, Md Zahangir
author_sort Salihu, Aliyu
title Thermostable lipases: an overview of production, purification and characterization
title_short Thermostable lipases: an overview of production, purification and characterization
title_full Thermostable lipases: an overview of production, purification and characterization
title_fullStr Thermostable lipases: an overview of production, purification and characterization
title_full_unstemmed Thermostable lipases: an overview of production, purification and characterization
title_sort thermostable lipases: an overview of production, purification and characterization
publisher Oriental Scientific Publishing Company
publishDate 2014
url http://irep.iium.edu.my/40933/
http://irep.iium.edu.my/40933/
http://irep.iium.edu.my/40933/
http://irep.iium.edu.my/40933/1/Paper-aliyu.pdf
http://irep.iium.edu.my/40933/4/40933-Thermostable%20lipases_SCOPUS.pdf
first_indexed 2023-09-18T20:58:39Z
last_indexed 2023-09-18T20:58:39Z
_version_ 1777410474629922816