Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells

INTRODUCTION: Lung cancer is the most common type of cancer which the mortality rate increases year by year. Therapeutic drug could not control the progression of cancer effectively and it is also contributing to the side effects. Hence, alternative strategy such as chemoprevention using natural pro...

Full description

Bibliographic Details
Main Authors:	Nik Abdul Malek, Nik Nurasyikin, Abdul Ghani, Radiah
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/40683/ http://irep.iium.edu.my/40683/ http://irep.iium.edu.my/40683/1/chemopreventive-_Radiah.pdf

id	iium-40683
recordtype	eprints
spelling	iium-406832015-02-10T02:07:06Z http://irep.iium.edu.my/40683/ Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells Nik Abdul Malek, Nik Nurasyikin Abdul Ghani, Radiah RB Pathology INTRODUCTION: Lung cancer is the most common type of cancer which the mortality rate increases year by year. Therapeutic drug could not control the progression of cancer effectively and it is also contributing to the side effects. Hence, alternative strategy such as chemoprevention using natural product should be investigated. P. granatumor pomegranate is one of the prophetic foods which has a potential to be a chemopreventive agent. It is rich in phytochemicals and has been used as medicine. This study is aimed to evaluate the anti-proliferative effect of the pomegranate on the growth of human lung carcinoma A594 cells. MATERIALS AND METHODS: The cytotoxicity test had been conducted by using MTT assay. The effect of pomegranate juice on A549 cancer cells growth and protein content were evaluated by trypan blue exclusion assay and Lowry assay, respectively. RESULTS AND DISCUSSION: The result showed that various concentrations of pomegranate juice treatment range from 0.0 to 3.0%resulted in a dose dependent inhibition of A549 cell growth. The inhibitory concentration, IC50 value was 0.8% after 48hr exposure, and 1.5% after 72hr and 96hr exposure. At the concentration of 1%, inhibition of growth was observed by decreased in cell number and protein content. From the results, the decreased of the protein content was lowered as compared to the untreated A549 cells. CONCLUSION: This preliminary study shown that, the pomegranate juice could inhibit the proliferation of A549 cancer cells. Hence, it could be a good strategy to prevent or slow the growth of cells. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/40683/1/chemopreventive-_Radiah.pdf Nik Abdul Malek, Nik Nurasyikin and Abdul Ghani, Radiah (2014) Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells. In: K.A.H.S. Research Week 2014, 17-21 Nov. 2014, KAHS IIUM, Kuantan, Pahang. http://www.iium.edu.my/kahs/events/kahs-research-week-2014
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Nik Abdul Malek, Nik Nurasyikin Abdul Ghani, Radiah Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells
description	INTRODUCTION: Lung cancer is the most common type of cancer which the mortality rate increases year by year. Therapeutic drug could not control the progression of cancer effectively and it is also contributing to the side effects. Hence, alternative strategy such as chemoprevention using natural product should be investigated. P. granatumor pomegranate is one of the prophetic foods which has a potential to be a chemopreventive agent. It is rich in phytochemicals and has been used as medicine. This study is aimed to evaluate the anti-proliferative effect of the pomegranate on the growth of human lung carcinoma A594 cells. MATERIALS AND METHODS: The cytotoxicity test had been conducted by using MTT assay. The effect of pomegranate juice on A549 cancer cells growth and protein content were evaluated by trypan blue exclusion assay and Lowry assay, respectively. RESULTS AND DISCUSSION: The result showed that various concentrations of pomegranate juice treatment range from 0.0 to 3.0%resulted in a dose dependent inhibition of A549 cell growth. The inhibitory concentration, IC50 value was 0.8% after 48hr exposure, and 1.5% after 72hr and 96hr exposure. At the concentration of 1%, inhibition of growth was observed by decreased in cell number and protein content. From the results, the decreased of the protein content was lowered as compared to the untreated A549 cells. CONCLUSION: This preliminary study shown that, the pomegranate juice could inhibit the proliferation of A549 cancer cells. Hence, it could be a good strategy to prevent or slow the growth of cells.
format	Conference or Workshop Item
author	Nik Abdul Malek, Nik Nurasyikin Abdul Ghani, Radiah
author_facet	Nik Abdul Malek, Nik Nurasyikin Abdul Ghani, Radiah
author_sort	Nik Abdul Malek, Nik Nurasyikin
title	Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells
title_short	Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells
title_full	Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells
title_fullStr	Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells
title_full_unstemmed	Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells
title_sort	chemopreventive effect of punica granatum in human lung carcinoma a549 cells
publishDate	2014
url	http://irep.iium.edu.my/40683/ http://irep.iium.edu.my/40683/ http://irep.iium.edu.my/40683/1/chemopreventive-_Radiah.pdf
first_indexed	2023-09-18T20:58:21Z
last_indexed	2023-09-18T20:58:21Z
_version_	1777410456303960064

Chemopreventive effect of Punica granatum in human lung carcinoma A549 cells

Similar Items