In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae)

In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae)

Objective: To screen the antioxidant, cholinesterase and tyrosinase enzymatic inhibition activities of the leaves and heartwood of Calophyllum symingtonianum (C. symingtonianum), and the bark of Calophyllum depressinervosum (C. depressinervosum). Methods: Samples of leaves and heartwood of C. symin...

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Main Authors: Aminudin, Nurul Iman, Ahmad, Farediah, Bakhtiar, M. Taher
Format: Article
Language:English
Published: Coastal Life Medicine Media co., Limited 2015
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/40630/
http://irep.iium.edu.my/40630/
http://irep.iium.edu.my/40630/
http://irep.iium.edu.my/40630/1/JLCM_Nurul_Iman.pdf
id iium-40630
recordtype eprints
spelling iium-406302015-01-29T08:35:05Z http://irep.iium.edu.my/40630/ In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae) Aminudin, Nurul Iman Ahmad, Farediah Bakhtiar, M. Taher RS Pharmacy and materia medica Objective: To screen the antioxidant, cholinesterase and tyrosinase enzymatic inhibition activities of the leaves and heartwood of Calophyllum symingtonianum (C. symingtonianum), and the bark of Calophyllum depressinervosum (C. depressinervosum). Methods: Samples of leaves and heartwood of C. symingtonianum and bark of C. depressinervosum were tested for their total phenolic content and in vitro antioxidant assay by 2,2-diphenyl-1-picrylhydrazyl radical scavenging and β-carotene bleaching. Cholinesterase inhibition by Ellman’s method and tyrosinase inhibition using L-3,4-dihydroxyphenylalanine as a substrate were also tested. Results: All methanol extracts were found to exhibit strong 2,2-diphenyl-1-picrylhydrazyl radical scavenging effects. Extracts from the heartwood of C. symingtonianum gave a low IC50 (5.17±0.04) μg/mL followed by bark of C. depressinervosum [(7.30±0.14) μg/mL] and C. symingtonianum leaves [(15.70±1.43) μg/mL]. The methanol extract of C. depressinervosum bark showed 95.08% inhibition of β-carotene bleaching. All extracts showed moderate inhibition towards tyrosinase activity with an IC50 of more than 100 μg/mL. The methanol extract of C. depressinervosum stem bark showed the highest inhibition (78.46%) against butyrylcholinesterase. Conclusions: These results showed that both Calophyllum species are potential sources of antioxidant and cholinesterase inhibitors. Further study is needed for the isolation and characterization of the active metabolites responsible for both activities. Coastal Life Medicine Media co., Limited 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/40630/1/JLCM_Nurul_Iman.pdf Aminudin, Nurul Iman and Ahmad, Farediah and Bakhtiar, M. Taher (2015) In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae). Journal of Coastal Life Medicine, 3 (2). pp. 126-131. ISSN 2309-6152 (O), 2309-5288 (P) http://www.jclmm.com/zz/20152.htm 10.12980/JCLM.3.2015JCLM-2014-0115
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Aminudin, Nurul Iman
Ahmad, Farediah
Bakhtiar, M. Taher
In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae)
description Objective: To screen the antioxidant, cholinesterase and tyrosinase enzymatic inhibition activities of the leaves and heartwood of Calophyllum symingtonianum (C. symingtonianum), and the bark of Calophyllum depressinervosum (C. depressinervosum). Methods: Samples of leaves and heartwood of C. symingtonianum and bark of C. depressinervosum were tested for their total phenolic content and in vitro antioxidant assay by 2,2-diphenyl-1-picrylhydrazyl radical scavenging and β-carotene bleaching. Cholinesterase inhibition by Ellman’s method and tyrosinase inhibition using L-3,4-dihydroxyphenylalanine as a substrate were also tested. Results: All methanol extracts were found to exhibit strong 2,2-diphenyl-1-picrylhydrazyl radical scavenging effects. Extracts from the heartwood of C. symingtonianum gave a low IC50 (5.17±0.04) μg/mL followed by bark of C. depressinervosum [(7.30±0.14) μg/mL] and C. symingtonianum leaves [(15.70±1.43) μg/mL]. The methanol extract of C. depressinervosum bark showed 95.08% inhibition of β-carotene bleaching. All extracts showed moderate inhibition towards tyrosinase activity with an IC50 of more than 100 μg/mL. The methanol extract of C. depressinervosum stem bark showed the highest inhibition (78.46%) against butyrylcholinesterase. Conclusions: These results showed that both Calophyllum species are potential sources of antioxidant and cholinesterase inhibitors. Further study is needed for the isolation and characterization of the active metabolites responsible for both activities.
format Article
author Aminudin, Nurul Iman
Ahmad, Farediah
Bakhtiar, M. Taher
author_facet Aminudin, Nurul Iman
Ahmad, Farediah
Bakhtiar, M. Taher
author_sort Aminudin, Nurul Iman
title In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae)
title_short In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae)
title_full In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae)
title_fullStr In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae)
title_full_unstemmed In vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae)
title_sort in vitro antioxidant, cholinesterase and tyrosinase inhibitory activities of calophyllum symingtonianum and calophyllum depressinervosum (guttiferae)
publisher Coastal Life Medicine Media co., Limited
publishDate 2015
url http://irep.iium.edu.my/40630/
http://irep.iium.edu.my/40630/
http://irep.iium.edu.my/40630/
http://irep.iium.edu.my/40630/1/JLCM_Nurul_Iman.pdf
first_indexed 2023-09-18T20:58:18Z
last_indexed 2023-09-18T20:58:18Z
_version_ 1777410452911816704

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