Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography

Ficus deltoidea (FD) is one of the native plants widely distributed in several countries in Southeast Asia. Previous studies have shown that FD leaf possess antinociceptive, wound healing and antioxidant properties. These beneficial effects have been attributed to the presence of primary and seconda...

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Main Authors:	Shafaei, Armaghan, F. F. A. Aisha, Abdalrahim, Ahmad Siddiqui, Mohammad Jamshed, Ismail, Zhari
Format:	Article
Language:	English English
Published:	Wolters Kluwer 2015
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/40620/ http://irep.iium.edu.my/40620/ http://irep.iium.edu.my/40620/1/Pharmcog_Res-2015-_Citrolline.pdf http://irep.iium.edu.my/40620/4/Pharmacognosy_research_2015_Citrolline.pdf

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recordtype	eprints
spelling	iium-406202017-11-23T08:34:26Z http://irep.iium.edu.my/40620/ Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography Shafaei, Armaghan F. F. A. Aisha, Abdalrahim Ahmad Siddiqui, Mohammad Jamshed Ismail, Zhari QD Chemistry Ficus deltoidea (FD) is one of the native plants widely distributed in several countries in Southeast Asia. Previous studies have shown that FD leaf possess antinociceptive, wound healing and antioxidant properties. These beneficial effects have been attributed to the presence of primary and secondary metabolites such as polyphenols, amino acids and flavonoids. Objective: The aim was to develop a reverse phase high-performance liquid chromatography method with ultraviolet detection that involves precolumn derivatisation with O-phthaladehyde for simultaneous analysis of two amino acids L-citrulline and L-arginine in FD leaf extracts. Materials and Methods: An isocratic elution program consisting of methanol: acetonitrile: Water at 45:45:10 v/v (solvent A) and 0.1 M phosphate buffer pH 7.5 (solvent B) at A: B v/v ratio of 80:20 on Zorbax Eclipse C18 SB-Aq column (250 × 4.6 mm, 5 μm) were used. The flow rate was set at 1 ml/min and detection was carried out at 338 nm with 30 min separation time. Results: Good linearity for L-citrulline and L-arginine was obtained in the range 0.1-1000 μg/ml at R2 ≥ 0.998. The limit of detection and limit of quantification values for both L-citrulline and L-arginine were 1 and 5 μg/ml, respectively. The average of recoveries was in the range 94.94-101.95%, with relative standard deviation (%RSD) less than 3%. Intra- and inter-day precision was in the range 96.36-102.43% with RSD less than 2%. Conclusion: All validation parameters of the developed method indicate the method is reliable and efficient for simultaneous determination of L-citrulline and L-arginine for routine analysis of FD. Wolters Kluwer 2015-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/40620/1/Pharmcog_Res-2015-_Citrolline.pdf application/pdf en http://irep.iium.edu.my/40620/4/Pharmacognosy_research_2015_Citrolline.pdf Shafaei, Armaghan and F. F. A. Aisha, Abdalrahim and Ahmad Siddiqui, Mohammad Jamshed and Ismail, Zhari (2015) Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography. Pharmacognosy Research, 7 (1). pp. 32-37. ISSN 0974-8490 DOI: 10.4103/0974-8490.147196
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Shafaei, Armaghan F. F. A. Aisha, Abdalrahim Ahmad Siddiqui, Mohammad Jamshed Ismail, Zhari Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography
description	Ficus deltoidea (FD) is one of the native plants widely distributed in several countries in Southeast Asia. Previous studies have shown that FD leaf possess antinociceptive, wound healing and antioxidant properties. These beneficial effects have been attributed to the presence of primary and secondary metabolites such as polyphenols, amino acids and flavonoids. Objective: The aim was to develop a reverse phase high-performance liquid chromatography method with ultraviolet detection that involves precolumn derivatisation with O-phthaladehyde for simultaneous analysis of two amino acids L-citrulline and L-arginine in FD leaf extracts. Materials and Methods: An isocratic elution program consisting of methanol: acetonitrile: Water at 45:45:10 v/v (solvent A) and 0.1 M phosphate buffer pH 7.5 (solvent B) at A: B v/v ratio of 80:20 on Zorbax Eclipse C18 SB-Aq column (250 × 4.6 mm, 5 μm) were used. The flow rate was set at 1 ml/min and detection was carried out at 338 nm with 30 min separation time. Results: Good linearity for L-citrulline and L-arginine was obtained in the range 0.1-1000 μg/ml at R2 ≥ 0.998. The limit of detection and limit of quantification values for both L-citrulline and L-arginine were 1 and 5 μg/ml, respectively. The average of recoveries was in the range 94.94-101.95%, with relative standard deviation (%RSD) less than 3%. Intra- and inter-day precision was in the range 96.36-102.43% with RSD less than 2%. Conclusion: All validation parameters of the developed method indicate the method is reliable and efficient for simultaneous determination of L-citrulline and L-arginine for routine analysis of FD.
format	Article
author	Shafaei, Armaghan F. F. A. Aisha, Abdalrahim Ahmad Siddiqui, Mohammad Jamshed Ismail, Zhari
author_facet	Shafaei, Armaghan F. F. A. Aisha, Abdalrahim Ahmad Siddiqui, Mohammad Jamshed Ismail, Zhari
author_sort	Shafaei, Armaghan
title	Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography
title_short	Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography
title_full	Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography
title_fullStr	Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography
title_full_unstemmed	Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography
title_sort	analysis of l-citrulline and l-arginine in ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography
publisher	Wolters Kluwer
publishDate	2015
url	http://irep.iium.edu.my/40620/ http://irep.iium.edu.my/40620/ http://irep.iium.edu.my/40620/1/Pharmcog_Res-2015-_Citrolline.pdf http://irep.iium.edu.my/40620/4/Pharmacognosy_research_2015_Citrolline.pdf
first_indexed	2023-09-18T20:58:17Z
last_indexed	2023-09-18T20:58:17Z
_version_	1777410452001652736

Analysis of L-citrulline and L-arginine in Ficus deltoidea leaf extracts by reverse phase high performance liquid chromatography

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