Immobilization of lipase enzyme by low cost material: a statistical approach

Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (AC) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy...

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Main Authors:	Alam, Md. Zahangir, Asih, Devi Ratna, Salleh, Md. Noor
Format:	Conference or Workshop Item
Language:	English
Published:	World Academy of Science, Engineering and Technology 2014
Subjects:	TP Chemical technology
Online Access:	http://irep.iium.edu.my/40458/ http://irep.iium.edu.my/40458/ http://irep.iium.edu.my/40458/1/Paper-Canada.pdf

id	iium-40458
recordtype	eprints
spelling	iium-404582018-06-12T07:45:09Z http://irep.iium.edu.my/40458/ Immobilization of lipase enzyme by low cost material: a statistical approach Alam, Md. Zahangir Asih, Devi Ratna Salleh, Md. Noor TP Chemical technology Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (AC) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy based on a statistical experimental design, including one-factor-at-a-time (OFAT) method was used to determine the equilibrium time. Three components influencing lipase immobilization were optimized by the response surface methodology (RSM) based on the face-centered central composite design (FCCCD). On the statistical analysis of the results, the optimum enzyme concentration loading, agitation rate and carbon active dosage were found to be 30 U/ml, 300 rpm and 8 g/L respectively, with a maximum immobilization activity of 3732.9 U/gAC after 2 hrs of immobilization. Analysis of variance (ANOVA) showed a high regression coefficient (R 2) of 0.999, which indicated a satisfactory fit of the model with the experimental data. The parameters were statistically significant at p<0.05. World Academy of Science, Engineering and Technology 2014-06 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/40458/1/Paper-Canada.pdf Alam, Md. Zahangir and Asih, Devi Ratna and Salleh, Md. Noor (2014) Immobilization of lipase enzyme by low cost material: a statistical approach. In: International Science Conference, 16-17 June 2014, Toronto, Canada. http://www.internationalscienceconference.org
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Alam, Md. Zahangir Asih, Devi Ratna Salleh, Md. Noor Immobilization of lipase enzyme by low cost material: a statistical approach
description	Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (AC) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy based on a statistical experimental design, including one-factor-at-a-time (OFAT) method was used to determine the equilibrium time. Three components influencing lipase immobilization were optimized by the response surface methodology (RSM) based on the face-centered central composite design (FCCCD). On the statistical analysis of the results, the optimum enzyme concentration loading, agitation rate and carbon active dosage were found to be 30 U/ml, 300 rpm and 8 g/L respectively, with a maximum immobilization activity of 3732.9 U/gAC after 2 hrs of immobilization. Analysis of variance (ANOVA) showed a high regression coefficient (R 2) of 0.999, which indicated a satisfactory fit of the model with the experimental data. The parameters were statistically significant at p<0.05.
format	Conference or Workshop Item
author	Alam, Md. Zahangir Asih, Devi Ratna Salleh, Md. Noor
author_facet	Alam, Md. Zahangir Asih, Devi Ratna Salleh, Md. Noor
author_sort	Alam, Md. Zahangir
title	Immobilization of lipase enzyme by low cost material: a statistical approach
title_short	Immobilization of lipase enzyme by low cost material: a statistical approach
title_full	Immobilization of lipase enzyme by low cost material: a statistical approach
title_fullStr	Immobilization of lipase enzyme by low cost material: a statistical approach
title_full_unstemmed	Immobilization of lipase enzyme by low cost material: a statistical approach
title_sort	immobilization of lipase enzyme by low cost material: a statistical approach
publisher	World Academy of Science, Engineering and Technology
publishDate	2014
url	http://irep.iium.edu.my/40458/ http://irep.iium.edu.my/40458/ http://irep.iium.edu.my/40458/1/Paper-Canada.pdf
first_indexed	2023-09-18T20:58:03Z
last_indexed	2023-09-18T20:58:03Z
_version_	1777410437252382720

Immobilization of lipase enzyme by low cost material: a statistical approach

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