Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells

Agarwood or Gaharu by definition is the resin impregnated heartwood from Aquilaria species that produces unique aromatic scent when burnt. apart from religious rituals and perfumes, agarwood has also been used as traditional medicines leading to the interest of this study which is to screen for anti...

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Main Authors:	Hashim, Yumi Zuhanis Has-Yun, Abbas, Phirdaous
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/40224/ http://irep.iium.edu.my/40224/1/Hashim_et_al_ICBWI_2014.pdf

id	iium-40224
recordtype	eprints
spelling	iium-402242015-06-18T00:33:18Z http://irep.iium.edu.my/40224/ Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells Hashim, Yumi Zuhanis Has-Yun Abbas, Phirdaous Q Science (General) Agarwood or Gaharu by definition is the resin impregnated heartwood from Aquilaria species that produces unique aromatic scent when burnt. apart from religious rituals and perfumes, agarwood has also been used as traditional medicines leading to the interest of this study which is to screen for anti-cancer properties to further support the traditional medicine claims. This present study aims to investigate the potential anto-cancer activity of un-inoculated A.sub-integra agarwood branch against MCF-7 breast cancer cells and screen for the most influential extraction parameters. Experimental design was generated using the MODDE software from SIMCA-P+ V12.0.1. Parameters included in the design were temperature °C, time (hour), igitation speed (rpm), and ratio of sample to the solvent (g/ml). Sulforhodamine B (SRB) assay was used to determine the potential cytotoxic effects of ABE against MCF-7 breast cancer cells. It was found that ethanolic-extract of agarwood branch (ABE) from experimental run 11 with 12 hours extraction time, 50 °C temperature, 100 rpm and 1:20 ratio of solid to extraction solvent gave the highest yield of 21.3% (g/g) of ABE from 3 gram of sample material. regression analysis found that agitation speed has significant negative effects. Meanwhile, run 16 (extraction time of 24hours, 50 °C extraction temperature, agitation speed of 200rpm and 1:20 solid to extraction solvent ratio) gave the lowest IC 50 of 8 ug/ml against MCF-7 cells. In conclusion, results from this study were able to confirm the anto-cancer effects of un-inoculated agarwood branch. More studies are warranted to elucidate the mechanism of agarwood extract in cancer prevention and therapeutics. This will further add value to the currently growing agarwood industry. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/40224/1/Hashim_et_al_ICBWI_2014.pdf Hashim, Yumi Zuhanis Has-Yun and Abbas, Phirdaous (2014) Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells. In: 5th International Conference on Biotechnology for the Wellness Industry, 10th-11th June 2014, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Hashim, Yumi Zuhanis Has-Yun Abbas, Phirdaous Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells
description	Agarwood or Gaharu by definition is the resin impregnated heartwood from Aquilaria species that produces unique aromatic scent when burnt. apart from religious rituals and perfumes, agarwood has also been used as traditional medicines leading to the interest of this study which is to screen for anti-cancer properties to further support the traditional medicine claims. This present study aims to investigate the potential anto-cancer activity of un-inoculated A.sub-integra agarwood branch against MCF-7 breast cancer cells and screen for the most influential extraction parameters. Experimental design was generated using the MODDE software from SIMCA-P+ V12.0.1. Parameters included in the design were temperature °C, time (hour), igitation speed (rpm), and ratio of sample to the solvent (g/ml). Sulforhodamine B (SRB) assay was used to determine the potential cytotoxic effects of ABE against MCF-7 breast cancer cells. It was found that ethanolic-extract of agarwood branch (ABE) from experimental run 11 with 12 hours extraction time, 50 °C temperature, 100 rpm and 1:20 ratio of solid to extraction solvent gave the highest yield of 21.3% (g/g) of ABE from 3 gram of sample material. regression analysis found that agitation speed has significant negative effects. Meanwhile, run 16 (extraction time of 24hours, 50 °C extraction temperature, agitation speed of 200rpm and 1:20 solid to extraction solvent ratio) gave the lowest IC 50 of 8 ug/ml against MCF-7 cells. In conclusion, results from this study were able to confirm the anto-cancer effects of un-inoculated agarwood branch. More studies are warranted to elucidate the mechanism of agarwood extract in cancer prevention and therapeutics. This will further add value to the currently growing agarwood industry.
format	Conference or Workshop Item
author	Hashim, Yumi Zuhanis Has-Yun Abbas, Phirdaous
author_facet	Hashim, Yumi Zuhanis Has-Yun Abbas, Phirdaous
author_sort	Hashim, Yumi Zuhanis Has-Yun
title	Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells
title_short	Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells
title_full	Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells
title_fullStr	Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells
title_full_unstemmed	Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells
title_sort	anti-cancer activity of un-inoculated agarwood branch against mcf-7 breast cancer cells
publishDate	2014
url	http://irep.iium.edu.my/40224/ http://irep.iium.edu.my/40224/1/Hashim_et_al_ICBWI_2014.pdf
first_indexed	2023-09-18T20:57:42Z
last_indexed	2023-09-18T20:57:42Z
_version_	1777410414516109312

Anti-cancer activity of un-inoculated agarwood branch against MCF-7 breast cancer cells

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