Improvement of phytase properties by rational design intended for animal feed

For application in animal feed, a phytase of interest must have high activity in the broad range prevalent industrial condition and in digestive tract of the animal. The present investigation describes approaches to rationally engineer a phytase with better activity profiles. Computer modelling is...

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Main Authors:	Samsudin, Nurhusna, Noorbatcha, Ibrahim Ali, Amid, Azura, Mohd. Salleh, Hamzah
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/4019/ http://irep.iium.edu.my/4019/1/IRIIE_2010_Abstract_Phytase_P201.pdf

id	iium-4019
recordtype	eprints
spelling	iium-40192012-04-20T00:10:57Z http://irep.iium.edu.my/4019/ Improvement of phytase properties by rational design intended for animal feed Samsudin, Nurhusna Noorbatcha, Ibrahim Ali Amid, Azura Mohd. Salleh, Hamzah TP248.13 Biotechnology For application in animal feed, a phytase of interest must have high activity in the broad range prevalent industrial condition and in digestive tract of the animal. The present investigation describes approaches to rationally engineer a phytase with better activity profiles. Computer modelling is used to identify and examine active site of phyFAUIA1 phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the binding strength before and after mutation. From the directive results of computational studies, point mutation was introduced by site directed mutagenesis using polymerase chain reaction (PCR). Mutagenesis is achieved by two steps PCR procedure. Four primers were designed and synthesized: two primers contain mutation at the point of interest which is complement to each other and the other two primers were designed for unique restriction sites. Several numbers of single, double as well as triple mutations have been introduced in phyFAUIA1 gene, and the mutant enzymes will be characterized and compared to computational results. 2010-01-26 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/4019/1/IRIIE_2010_Abstract_Phytase_P201.pdf Samsudin, Nurhusna and Noorbatcha, Ibrahim Ali and Amid, Azura and Mohd. Salleh, Hamzah (2010) Improvement of phytase properties by rational design intended for animal feed. In: IIUM Research, Innovation & Invention Exhibition, (IRIIE 2010), 26 - 27 January 2010, IIUM.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Samsudin, Nurhusna Noorbatcha, Ibrahim Ali Amid, Azura Mohd. Salleh, Hamzah Improvement of phytase properties by rational design intended for animal feed
description	For application in animal feed, a phytase of interest must have high activity in the broad range prevalent industrial condition and in digestive tract of the animal. The present investigation describes approaches to rationally engineer a phytase with better activity profiles. Computer modelling is used to identify and examine active site of phyFAUIA1 phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the binding strength before and after mutation. From the directive results of computational studies, point mutation was introduced by site directed mutagenesis using polymerase chain reaction (PCR). Mutagenesis is achieved by two steps PCR procedure. Four primers were designed and synthesized: two primers contain mutation at the point of interest which is complement to each other and the other two primers were designed for unique restriction sites. Several numbers of single, double as well as triple mutations have been introduced in phyFAUIA1 gene, and the mutant enzymes will be characterized and compared to computational results.
format	Conference or Workshop Item
author	Samsudin, Nurhusna Noorbatcha, Ibrahim Ali Amid, Azura Mohd. Salleh, Hamzah
author_facet	Samsudin, Nurhusna Noorbatcha, Ibrahim Ali Amid, Azura Mohd. Salleh, Hamzah
author_sort	Samsudin, Nurhusna
title	Improvement of phytase properties by rational design intended for animal feed
title_short	Improvement of phytase properties by rational design intended for animal feed
title_full	Improvement of phytase properties by rational design intended for animal feed
title_fullStr	Improvement of phytase properties by rational design intended for animal feed
title_full_unstemmed	Improvement of phytase properties by rational design intended for animal feed
title_sort	improvement of phytase properties by rational design intended for animal feed
publishDate	2010
url	http://irep.iium.edu.my/4019/ http://irep.iium.edu.my/4019/1/IRIIE_2010_Abstract_Phytase_P201.pdf
first_indexed	2023-09-18T20:12:04Z
last_indexed	2023-09-18T20:12:04Z
_version_	1777407543869440000

Improvement of phytase properties by rational design intended for animal feed

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