Quantitative protein expression studies of Malaysia broiler and organic chicken skin

Antibiotic treatment is one of the methods used in animal farming. Most of it was given to treat disease and increase growth in animal. Despite its benefit, there are concerns about the residue of antibiotic, due to the health related problems that includes antibiotic resistance and toxicity. Two ty...

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Main Authors:	Mohamad Zani, Radiayatul Akma, Muhamad Bunnori, Noraslinda
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/40037/ http://irep.iium.edu.my/40037/3/40037.pdf

id	iium-40037
recordtype	eprints
spelling	iium-400372016-10-27T02:28:34Z http://irep.iium.edu.my/40037/ Quantitative protein expression studies of Malaysia broiler and organic chicken skin Mohamad Zani, Radiayatul Akma Muhamad Bunnori, Noraslinda TP248.13 Biotechnology Antibiotic treatment is one of the methods used in animal farming. Most of it was given to treat disease and increase growth in animal. Despite its benefit, there are concerns about the residue of antibiotic, due to the health related problems that includes antibiotic resistance and toxicity. Two types of chicken sin samples, which are Broilers(B) and organic chicken (O), were examined by using proteomic strategy. The purpose of this study is to analyse and compare the protein expression of chicken skin between broiler and organic chicken. Chicken samples were homogenized in liquid nitrogen and sample buffer. The supernatant used to determine protein concentration in two types of chicken samples by Braford technique. Then the protein samples were examined by using SDS-PAGE and further resolved by using two-dimensional gel electrophoresis (2D-PAGE). The gels were viewed using GD-800Calibrated Densitometer (Bio-Rad) using Quantity One 1D Analysis software version 4.5 and PD Quest 2D Analysis Software (Bio-Rad). The protein interest spots in second dimensional gel electrophoresis matched to ExPASy SWISS-2DPAGE database as reference to determine what type of protein that were expressed. Results showed that the mean value of protein concentrations in broiler and organic chicken skin were 6.592 ug/uL and 7.296 ug/uL respectively. From the result of 2D-PAGE, protein spotted on gel near pH 4.0 and Mw 50-75 kDa and spot on pH 7.0 and Mw 10-15 kDa was found in different intensity between both samples and predicted as heat shock protein and actin respectively. In conclusion, there were various different of protein expression detected in skin sample between broilers and organic chicken. 2014-05 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/40037/3/40037.pdf Mohamad Zani, Radiayatul Akma and Muhamad Bunnori, Noraslinda (2014) Quantitative protein expression studies of Malaysia broiler and organic chicken skin. In: 5th Malaysian Symposium of Biomedical Science, 10th-11th May 2014, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohamad Zani, Radiayatul Akma Muhamad Bunnori, Noraslinda Quantitative protein expression studies of Malaysia broiler and organic chicken skin
description	Antibiotic treatment is one of the methods used in animal farming. Most of it was given to treat disease and increase growth in animal. Despite its benefit, there are concerns about the residue of antibiotic, due to the health related problems that includes antibiotic resistance and toxicity. Two types of chicken sin samples, which are Broilers(B) and organic chicken (O), were examined by using proteomic strategy. The purpose of this study is to analyse and compare the protein expression of chicken skin between broiler and organic chicken. Chicken samples were homogenized in liquid nitrogen and sample buffer. The supernatant used to determine protein concentration in two types of chicken samples by Braford technique. Then the protein samples were examined by using SDS-PAGE and further resolved by using two-dimensional gel electrophoresis (2D-PAGE). The gels were viewed using GD-800Calibrated Densitometer (Bio-Rad) using Quantity One 1D Analysis software version 4.5 and PD Quest 2D Analysis Software (Bio-Rad). The protein interest spots in second dimensional gel electrophoresis matched to ExPASy SWISS-2DPAGE database as reference to determine what type of protein that were expressed. Results showed that the mean value of protein concentrations in broiler and organic chicken skin were 6.592 ug/uL and 7.296 ug/uL respectively. From the result of 2D-PAGE, protein spotted on gel near pH 4.0 and Mw 50-75 kDa and spot on pH 7.0 and Mw 10-15 kDa was found in different intensity between both samples and predicted as heat shock protein and actin respectively. In conclusion, there were various different of protein expression detected in skin sample between broilers and organic chicken.
format	Conference or Workshop Item
author	Mohamad Zani, Radiayatul Akma Muhamad Bunnori, Noraslinda
author_facet	Mohamad Zani, Radiayatul Akma Muhamad Bunnori, Noraslinda
author_sort	Mohamad Zani, Radiayatul Akma
title	Quantitative protein expression studies of Malaysia broiler and organic chicken skin
title_short	Quantitative protein expression studies of Malaysia broiler and organic chicken skin
title_full	Quantitative protein expression studies of Malaysia broiler and organic chicken skin
title_fullStr	Quantitative protein expression studies of Malaysia broiler and organic chicken skin
title_full_unstemmed	Quantitative protein expression studies of Malaysia broiler and organic chicken skin
title_sort	quantitative protein expression studies of malaysia broiler and organic chicken skin
publishDate	2014
url	http://irep.iium.edu.my/40037/ http://irep.iium.edu.my/40037/3/40037.pdf
first_indexed	2023-09-18T20:57:28Z
last_indexed	2023-09-18T20:57:28Z
_version_	1777410400427442176

Quantitative protein expression studies of Malaysia broiler and organic chicken skin

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