Molecular phylogeny of horseshoe crab

An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) wh...

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Main Authors:	Yunus, Kamaruzzaman, John, Akbar, Kasim, Zaleha, Khan Chowdhury, Ahmed Jalal
Format:	Article
Language:	English
Published:	Knowledgia Review, Malaysia 2011
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/3986/ http://irep.iium.edu.my/3986/ http://irep.iium.edu.my/3986/ http://irep.iium.edu.my/3986/1/302-309_2.pdf

id	iium-3986
recordtype	eprints
spelling	iium-39862017-12-07T05:23:27Z http://irep.iium.edu.my/3986/ Molecular phylogeny of horseshoe crab Yunus, Kamaruzzaman John, Akbar Kasim, Zaleha Khan Chowdhury, Ahmed Jalal QL Zoology An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) which include horseshoe crabs (Class: Merostomata), beetles (Class: Insecta), common crabs (Class: Melacostraca) and scorpion (Class: Arachnida) sequences. Constructed phylogram through comprehensive dry lab methodology using advanced software predictive tools showed that beetles are closely related with horseshoe crabs than common crabs (Class: Melacostraca). It was interesting to note that terrestrial Scorpion (Class: Arachnida) were distantly related to horseshoe crabs (Class: Merostomata). Phylogram and observed Genetic Distance (GD) data were also revealed that Limulus polyphemus was distantly related to all the other horseshoe crab species. Tachypleus gigas was closely related with Carcinoscorpius rotundicauda than with Tachypleus tridentatus. The observed mean Genetic Distance (GD) value was higher in 3rd codon position in all the selected group of organisms. Among the horseshoe crabs high GC content was observed in L. polyphemus (38.32%) and lowest was observed in T. tridentatus (32.35%). We conclude that COI sequencing (barcoding) could be used in identifying and delineating evolutionary relatedness with closely related species Knowledgia Review, Malaysia 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3986/1/302-309_2.pdf Yunus, Kamaruzzaman and John, Akbar and Kasim, Zaleha and Khan Chowdhury, Ahmed Jalal (2011) Molecular phylogeny of horseshoe crab. Asian Journal of Biotechnology, 3 (3). pp. 302-309. ISSN 1996-0700 (P) http://scialert.net/abstract/?doi=ajbkr.2011.302.309 10.3923/ajbkr.2011.302.309
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QL Zoology
spellingShingle	QL Zoology Yunus, Kamaruzzaman John, Akbar Kasim, Zaleha Khan Chowdhury, Ahmed Jalal Molecular phylogeny of horseshoe crab
description	An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) which include horseshoe crabs (Class: Merostomata), beetles (Class: Insecta), common crabs (Class: Melacostraca) and scorpion (Class: Arachnida) sequences. Constructed phylogram through comprehensive dry lab methodology using advanced software predictive tools showed that beetles are closely related with horseshoe crabs than common crabs (Class: Melacostraca). It was interesting to note that terrestrial Scorpion (Class: Arachnida) were distantly related to horseshoe crabs (Class: Merostomata). Phylogram and observed Genetic Distance (GD) data were also revealed that Limulus polyphemus was distantly related to all the other horseshoe crab species. Tachypleus gigas was closely related with Carcinoscorpius rotundicauda than with Tachypleus tridentatus. The observed mean Genetic Distance (GD) value was higher in 3rd codon position in all the selected group of organisms. Among the horseshoe crabs high GC content was observed in L. polyphemus (38.32%) and lowest was observed in T. tridentatus (32.35%). We conclude that COI sequencing (barcoding) could be used in identifying and delineating evolutionary relatedness with closely related species
format	Article
author	Yunus, Kamaruzzaman John, Akbar Kasim, Zaleha Khan Chowdhury, Ahmed Jalal
author_facet	Yunus, Kamaruzzaman John, Akbar Kasim, Zaleha Khan Chowdhury, Ahmed Jalal
author_sort	Yunus, Kamaruzzaman
title	Molecular phylogeny of horseshoe crab
title_short	Molecular phylogeny of horseshoe crab
title_full	Molecular phylogeny of horseshoe crab
title_fullStr	Molecular phylogeny of horseshoe crab
title_full_unstemmed	Molecular phylogeny of horseshoe crab
title_sort	molecular phylogeny of horseshoe crab
publisher	Knowledgia Review, Malaysia
publishDate	2011
url	http://irep.iium.edu.my/3986/ http://irep.iium.edu.my/3986/ http://irep.iium.edu.my/3986/ http://irep.iium.edu.my/3986/1/302-309_2.pdf
first_indexed	2023-09-18T20:12:00Z
last_indexed	2023-09-18T20:12:00Z
_version_	1777407540226686976

Molecular phylogeny of horseshoe crab

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