Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun)

The leaves of Coleus amboinicus from Indonesia (CAL-I) and from Malaysia, Coleus aromaticus (CAT-M), Pogostemon cablin (PC-M), Coleus blumei–red leaves (CBR-M), Coleus amboinicus– (CAL-M) Coleus blumei –purple leaves (CBP-M) were collected from different localities, freeze dried and extracted with a...

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Main Authors:	Khattak, Muhammad Muzaffar Ali Khan, Taher, Muhammad
Format:	Monograph
Language:	English
Published:	Submitted IIUM RMC 2011
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/3985/ http://irep.iium.edu.my/3985/1/DETAIL_END_OF_THE_PROJECT_REPORT_Torbungun.pdf

id	iium-3985
recordtype	eprints
spelling	iium-39852013-01-16T06:34:48Z http://irep.iium.edu.my/3985/ Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun) Khattak, Muhammad Muzaffar Ali Khan Taher, Muhammad TX341 Nutrition. Foods and food supply The leaves of Coleus amboinicus from Indonesia (CAL-I) and from Malaysia, Coleus aromaticus (CAT-M), Pogostemon cablin (PC-M), Coleus blumei–red leaves (CBR-M), Coleus amboinicus– (CAL-M) Coleus blumei –purple leaves (CBP-M) were collected from different localities, freeze dried and extracted with aqueous methanol. The biological activity in vitro, especially in relation to total phenolic & flavonoid contents, antioxidant and antimicrobial activities were studied. Total phenolic content was determined according to the Folin-Ciocalteu method whilst antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The antimicrobial activity of the extract was determined by making use of macro dilution and disc diffusion methods on two gram positive bacteria, two gram negative bacteria and on fungus as well. Furthermore, the toxicity was also assessed for the extracts by performing acute toxicity test. The phenolic content among the six Lamiaceae leaves extract showed significant difference (p<0.05) in result ranging from 55.21 - 95.17 mg GAE/g of dried samples. Pogostemon cablin (PC-M) had the highest content of phenolic followed by C. blumei (CBP-M). There were significant differences (p<0.05) of IC50 value of six Lamiaceae leaves extract ranging from 10.5 – 34.1 µg/ml. Among the species studied, Coleus amboinicus (CAL-M) and Pogostemon cablin (PC-M) showed higher antioxidant activity compared to the other leaves extracts. All leaves extracts showed activity at least against one strain of bacteria and result showed significant difference (p<0.05) between activities on the microorganism studied. On the contrary, all of the leaves extracts were not effective against C. albicans. The minimum inhibitory concentration (MIC) of all leaves extracts ranged from 1.0–2.0 mg/ml in inhibiting the growth of S. aureus, E. Coli, P. aeruginosa and B.subtilis. The acute toxicity test using C. Blumei leaves extract showed that there was no mortality of animals recorded at the highest dose of 5000 mg/kg body weight. This study shows that the extracts can be used as antioxidant and antimicrobial agent without having the toxic effect. Submitted IIUM RMC 2011 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/3985/1/DETAIL_END_OF_THE_PROJECT_REPORT_Torbungun.pdf Khattak, Muhammad Muzaffar Ali Khan and Taher, Muhammad (2011) Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun). Technical Report. Submitted IIUM RMC. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Khattak, Muhammad Muzaffar Ali Khan Taher, Muhammad Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun)
description	The leaves of Coleus amboinicus from Indonesia (CAL-I) and from Malaysia, Coleus aromaticus (CAT-M), Pogostemon cablin (PC-M), Coleus blumei–red leaves (CBR-M), Coleus amboinicus– (CAL-M) Coleus blumei –purple leaves (CBP-M) were collected from different localities, freeze dried and extracted with aqueous methanol. The biological activity in vitro, especially in relation to total phenolic & flavonoid contents, antioxidant and antimicrobial activities were studied. Total phenolic content was determined according to the Folin-Ciocalteu method whilst antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The antimicrobial activity of the extract was determined by making use of macro dilution and disc diffusion methods on two gram positive bacteria, two gram negative bacteria and on fungus as well. Furthermore, the toxicity was also assessed for the extracts by performing acute toxicity test. The phenolic content among the six Lamiaceae leaves extract showed significant difference (p<0.05) in result ranging from 55.21 - 95.17 mg GAE/g of dried samples. Pogostemon cablin (PC-M) had the highest content of phenolic followed by C. blumei (CBP-M). There were significant differences (p<0.05) of IC50 value of six Lamiaceae leaves extract ranging from 10.5 – 34.1 µg/ml. Among the species studied, Coleus amboinicus (CAL-M) and Pogostemon cablin (PC-M) showed higher antioxidant activity compared to the other leaves extracts. All leaves extracts showed activity at least against one strain of bacteria and result showed significant difference (p<0.05) between activities on the microorganism studied. On the contrary, all of the leaves extracts were not effective against C. albicans. The minimum inhibitory concentration (MIC) of all leaves extracts ranged from 1.0–2.0 mg/ml in inhibiting the growth of S. aureus, E. Coli, P. aeruginosa and B.subtilis. The acute toxicity test using C. Blumei leaves extract showed that there was no mortality of animals recorded at the highest dose of 5000 mg/kg body weight. This study shows that the extracts can be used as antioxidant and antimicrobial agent without having the toxic effect.
format	Monograph
author	Khattak, Muhammad Muzaffar Ali Khan Taher, Muhammad
author_facet	Khattak, Muhammad Muzaffar Ali Khan Taher, Muhammad
author_sort	Khattak, Muhammad Muzaffar Ali Khan
title	Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun)
title_short	Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun)
title_full	Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun)
title_fullStr	Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun)
title_full_unstemmed	Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun)
title_sort	bioactivity -guided isolation of antimicrobial agent from coleus amboinicus lour (torbangun)
publisher	Submitted IIUM RMC
publishDate	2011
url	http://irep.iium.edu.my/3985/ http://irep.iium.edu.my/3985/1/DETAIL_END_OF_THE_PROJECT_REPORT_Torbungun.pdf
first_indexed	2023-09-18T20:12:00Z
last_indexed	2023-09-18T20:12:00Z
_version_	1777407540068352000

Bioactivity -guided isolation of antimicrobial agent from Coleus amboinicus Lour (Torbangun)

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