Esterification for butyl butyrate formation using Candida cylindracea lipase produced from palm oil mill effluent supplemented medium
The ability of Candida cylindracea lipase produced using palm oil mill effluent (POME) as a basal medium to catal yze the esterification reaction for butyl butyrate formation was investi-gated. Butyric acid and n-butanol were used as substrates at different molar ratios. Different con-version yields...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/39799/ http://irep.iium.edu.my/39799/ http://irep.iium.edu.my/39799/ http://irep.iium.edu.my/39799/1/Paper-Arabian_j_Aliyu.pdf http://irep.iium.edu.my/39799/4/39799_Esterification%20for%20butyl%20butyrate%20formation_Scopus.pdf |
| Summary: | The ability of Candida cylindracea lipase produced using palm oil mill effluent (POME)
as a basal medium to catal yze the esterification reaction for butyl butyrate formation was investi-gated. Butyric acid and n-butanol were used as substrates at different molar ratios. Different con-version yields were observed according to the affinity of the produced lipase toward the substrates.
The n -butanol to butyric acid molar ratio of 8 and lipase concentration of 75 U/mg gave the highest
butyl butyrate formation of 63.33% based on the statistical optimization using face centered central
composite design (FCCCD) after 12 h reaction. The esterification potential of the POME based
lipase when compared with the commercial lipase from the same strain using the optimum levels
was found to show a similar pattern. It can be concluded therefore that the produced lipase pos-sesses appropriate characteristics to be used as a biocatalyst in the esterification reactions for butyl
butyrate formation |
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