Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application

An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa (N. sativa) oil in an emulsion to reduce gentamicin toxicity effect over prolonged use in osteo-infection treatment. This work aims to test the aqueous solubility and phys...

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Main Authors:	Yusof, Fathin Athirah, Yaacob, Khairul Ikhwan, Mohd Yusof, Nazri, Mohamed, Farahidah, Mohd Shafri, Mohd Affendi
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/39647/ http://irep.iium.edu.my/39647/1/ICIP_-_Fathin_Athirah_%28G1313984%29_horizontal2.pdf http://irep.iium.edu.my/39647/4/formulation_and_stability.pdf

id	iium-39647
recordtype	eprints
spelling	iium-396472015-01-14T02:00:16Z http://irep.iium.edu.my/39647/ Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application Yusof, Fathin Athirah Yaacob, Khairul Ikhwan Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi RM Therapeutics. Pharmacology An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa (N. sativa) oil in an emulsion to reduce gentamicin toxicity effect over prolonged use in osteo-infection treatment. This work aims to test the aqueous solubility and physicochemical properties of the emulsion. Four emulsions (emulsion A, B, C and D) had been formulated, with final concentration of gentamicin was made constant at 0.1% (w/v) whereas N. sativa oil concentration was varied between 32.5% (v/v) to 46.4% (v/v) in all formulations. Then, stability studies of all emulsion were performed by centrifugation at (5000rpm, 5 minutes), at different storage conditions (8oC, 25oC and 50oC), organoleptic characteristics, freeze-thawcycle, pH determination, particle size measurement, zeta-potential analysis, and pH titration analysis. Results showed no phase separation after centrifugation for freshly prepared emulsions. Storage at 8oC, all emulsions also showed no phase separation at all-time points. At 25oC storage condition, three formulations were stable at day 7 but phase separation was formed in all emulsions by day 14showed good stability at day 7 and all emulsions formed phase separation at day 14. No emulsions were stable in storage temperature of 50oC. The particle size of the emulsions increased with an increment ofN. sativa oil concentration. Zeta-potential analysis showed a range of -32.2 ± 0.15 mV to -48.0 ± 0.45 mV. When pH titration analysis was performed, the zeta potential indicated that the emulsion stability was affected by acidic conditions. We concluded that the use of gentamicin-N. Sativaemulsions must take into account the storage condition with preference of low temperature and fresh preparation at higher alkalinity and the lowest possibility of N. sativa oil. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/39647/1/ICIP_-_Fathin_Athirah_%28G1313984%29_horizontal2.pdf application/pdf en http://irep.iium.edu.my/39647/4/formulation_and_stability.pdf Yusof, Fathin Athirah and Yaacob, Khairul Ikhwan and Mohd Yusof, Nazri and Mohamed, Farahidah and Mohd Shafri, Mohd Affendi (2014) Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application. In: 1st International Conference of Industrial Pharmacy (ICIP 2014), 16th-17th Aug. 2014, Kuantan, Pahang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Yusof, Fathin Athirah Yaacob, Khairul Ikhwan Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application
description	An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa (N. sativa) oil in an emulsion to reduce gentamicin toxicity effect over prolonged use in osteo-infection treatment. This work aims to test the aqueous solubility and physicochemical properties of the emulsion. Four emulsions (emulsion A, B, C and D) had been formulated, with final concentration of gentamicin was made constant at 0.1% (w/v) whereas N. sativa oil concentration was varied between 32.5% (v/v) to 46.4% (v/v) in all formulations. Then, stability studies of all emulsion were performed by centrifugation at (5000rpm, 5 minutes), at different storage conditions (8oC, 25oC and 50oC), organoleptic characteristics, freeze-thawcycle, pH determination, particle size measurement, zeta-potential analysis, and pH titration analysis. Results showed no phase separation after centrifugation for freshly prepared emulsions. Storage at 8oC, all emulsions also showed no phase separation at all-time points. At 25oC storage condition, three formulations were stable at day 7 but phase separation was formed in all emulsions by day 14showed good stability at day 7 and all emulsions formed phase separation at day 14. No emulsions were stable in storage temperature of 50oC. The particle size of the emulsions increased with an increment ofN. sativa oil concentration. Zeta-potential analysis showed a range of -32.2 ± 0.15 mV to -48.0 ± 0.45 mV. When pH titration analysis was performed, the zeta potential indicated that the emulsion stability was affected by acidic conditions. We concluded that the use of gentamicin-N. Sativaemulsions must take into account the storage condition with preference of low temperature and fresh preparation at higher alkalinity and the lowest possibility of N. sativa oil.
format	Conference or Workshop Item
author	Yusof, Fathin Athirah Yaacob, Khairul Ikhwan Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi
author_facet	Yusof, Fathin Athirah Yaacob, Khairul Ikhwan Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi
author_sort	Yusof, Fathin Athirah
title	Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application
title_short	Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application
title_full	Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application
title_fullStr	Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application
title_full_unstemmed	Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application
title_sort	formulation and stability testing of gentamicin-nigella sativa emulsions for osteo-healing application
publishDate	2014
url	http://irep.iium.edu.my/39647/ http://irep.iium.edu.my/39647/1/ICIP_-_Fathin_Athirah_%28G1313984%29_horizontal2.pdf http://irep.iium.edu.my/39647/4/formulation_and_stability.pdf
first_indexed	2023-09-18T20:56:58Z
last_indexed	2023-09-18T20:56:58Z
_version_	1777410369256423424

Formulation and stability testing of gentamicin-Nigella sativa emulsions for osteo-healing application

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