Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis

Biofilm bacteria complicate treatment of osteomyelitis by providing resistant to antibiotics, requiring high doses of 10 to 100 times of standard antibiotic treatment. This high leads several adverse effects and toxicity. To overcome these challenges, Gentamicin and Nigella sativa, a natural produ...

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Main Authors:	Yaacob, Khairul Ikhwan, Yusof, Fatin Athirah, Mohd Yusof, Nazri, Mohamed, Farahidah, Mohd Shafri, Mohd Affendi
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	QR Microbiology R Medicine (General)
Online Access:	http://irep.iium.edu.my/39632/ http://irep.iium.edu.my/39632/ http://irep.iium.edu.my/39632/1/apccmi_poster_%28draft%292.pdf http://irep.iium.edu.my/39632/4/05-APCCMI-2014-Poster-27th-Nov-Abstract.pdf

id	iium-39632
recordtype	eprints
spelling	iium-396322015-01-14T01:10:34Z http://irep.iium.edu.my/39632/ Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis Yaacob, Khairul Ikhwan Yusof, Fatin Athirah Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi QR Microbiology R Medicine (General) Biofilm bacteria complicate treatment of osteomyelitis by providing resistant to antibiotics, requiring high doses of 10 to 100 times of standard antibiotic treatment. This high leads several adverse effects and toxicity. To overcome these challenges, Gentamicin and Nigella sativa, a natural product with antimicrobial property, are fused in an emulsion. Four emulsions (A,B,C, and D) (containing 0.1% w/v Gentamicin and N.sativa oil concentration between 32.5% v/v to 46.6% v/v) were tested against common osteomyelitic, gentamicin-resistant, biofilm bacteria namely S.aureus, S.epidermidis and P.aeruginosa. Test bacteria were isolated by sonicating clinical samples (Hospital Tengku Ampuan Afzan, Kuantan) and identified using colony morphology, biochemical test and API identification system (Bioemeriuex). Each strain was then tested for biofilm producing ability, followed by sensitivity testing to gentamicin. Antimicrobial susceptibility testing was performed using Disc Diffusion Assay by loading 20µl of test product in blank disc placed on a plate that had been spread with test bacteria. Zone of inhibition was measured after 24 hours incubation. Finally, biofilm formation inhibition was evaluated by transferring emulsions (20µl), test bacteria (100µl) and broth (80 l) per well of a 96-well culture plate and incubated for 24 hours at 37°C. After incubation, wells were decanted, washed 3 times with phosphate buffer solution, stained with 1% crystal violet before OD readings were collected using microplate reader. Results are analyzed using one way ANOVA. Emulsions were compared against N. sativa alone, Gentamicin alone, and untreated sample as controls. The result of disc diffusion, indicated by diameter of zone of inhibition, revealed that significant different are seen against Gentamicin (Tukey’s, p value < 0.05, all emulsions) and N. sativa alone (Tukey’s, p value < 0.05, emulsions A and C) against S.aureus and S.epidermidis. In inhibiting biofilm formation, both emulsions and N. sativa alone have significant activity in comparison to Gentamicin (Tukey’s, p value < 0.05). No activity was exhibited against P.aeruginosa. In conclusion, significantly improved anti-microbial and anti biofilm effects are seen against S.aureus and S.epidermidis but not P.aeruginosa, hence the emulsions have a good prospect to be used future as an effective treatment against some gentamicin-resistant, biofilm bacteria. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/39632/1/apccmi_poster_%28draft%292.pdf application/pdf en http://irep.iium.edu.my/39632/4/05-APCCMI-2014-Poster-27th-Nov-Abstract.pdf Yaacob, Khairul Ikhwan and Yusof, Fatin Athirah and Mohd Yusof, Nazri and Mohamed, Farahidah and Mohd Shafri, Mohd Affendi (2014) Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis. In: 15th Asia-Pacific Congress on Clinical Microbiology and Infection, 25-29th Dec. 2014, Sunway Convention Centre. (Unpublished) http://www.apccmi2014.org/poster-27slot
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology R Medicine (General)
spellingShingle	QR Microbiology R Medicine (General) Yaacob, Khairul Ikhwan Yusof, Fatin Athirah Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis
description	Biofilm bacteria complicate treatment of osteomyelitis by providing resistant to antibiotics, requiring high doses of 10 to 100 times of standard antibiotic treatment. This high leads several adverse effects and toxicity. To overcome these challenges, Gentamicin and Nigella sativa, a natural product with antimicrobial property, are fused in an emulsion. Four emulsions (A,B,C, and D) (containing 0.1% w/v Gentamicin and N.sativa oil concentration between 32.5% v/v to 46.6% v/v) were tested against common osteomyelitic, gentamicin-resistant, biofilm bacteria namely S.aureus, S.epidermidis and P.aeruginosa. Test bacteria were isolated by sonicating clinical samples (Hospital Tengku Ampuan Afzan, Kuantan) and identified using colony morphology, biochemical test and API identification system (Bioemeriuex). Each strain was then tested for biofilm producing ability, followed by sensitivity testing to gentamicin. Antimicrobial susceptibility testing was performed using Disc Diffusion Assay by loading 20µl of test product in blank disc placed on a plate that had been spread with test bacteria. Zone of inhibition was measured after 24 hours incubation. Finally, biofilm formation inhibition was evaluated by transferring emulsions (20µl), test bacteria (100µl) and broth (80 l) per well of a 96-well culture plate and incubated for 24 hours at 37°C. After incubation, wells were decanted, washed 3 times with phosphate buffer solution, stained with 1% crystal violet before OD readings were collected using microplate reader. Results are analyzed using one way ANOVA. Emulsions were compared against N. sativa alone, Gentamicin alone, and untreated sample as controls. The result of disc diffusion, indicated by diameter of zone of inhibition, revealed that significant different are seen against Gentamicin (Tukey’s, p value < 0.05, all emulsions) and N. sativa alone (Tukey’s, p value < 0.05, emulsions A and C) against S.aureus and S.epidermidis. In inhibiting biofilm formation, both emulsions and N. sativa alone have significant activity in comparison to Gentamicin (Tukey’s, p value < 0.05). No activity was exhibited against P.aeruginosa. In conclusion, significantly improved anti-microbial and anti biofilm effects are seen against S.aureus and S.epidermidis but not P.aeruginosa, hence the emulsions have a good prospect to be used future as an effective treatment against some gentamicin-resistant, biofilm bacteria.
format	Conference or Workshop Item
author	Yaacob, Khairul Ikhwan Yusof, Fatin Athirah Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi
author_facet	Yaacob, Khairul Ikhwan Yusof, Fatin Athirah Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi
author_sort	Yaacob, Khairul Ikhwan
title	Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis
title_short	Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis
title_full	Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis
title_fullStr	Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis
title_full_unstemmed	Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis
title_sort	synergistic effects of new gentamicin-n.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis
publishDate	2014
url	http://irep.iium.edu.my/39632/ http://irep.iium.edu.my/39632/ http://irep.iium.edu.my/39632/1/apccmi_poster_%28draft%292.pdf http://irep.iium.edu.my/39632/4/05-APCCMI-2014-Poster-27th-Nov-Abstract.pdf
first_indexed	2023-09-18T20:56:57Z
last_indexed	2023-09-18T20:56:57Z
_version_	1777410367938363392

Synergistic effects of new gentamicin-N.sativa emulsion against gentamicin-resistant, biofilm bacteria of osteomyelitis

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