Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application

An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa (N. sativa) oil in an emulsion to reduce gentamicin toxicity effect over prolonged use in osteo-infection treatment. This work aims to test the aqueous solubility and phys...

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Main Authors:	Mohd Shafri, Mohd Affendi, Mohamed, Farahidah, Yusof, Fatin Athirah, Yaacob, Khairul Ikhwan, Mohd Yusof, Nazri
Format:	Article
Language:	English English
Published:	Academic Sciences 2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/39609/ http://irep.iium.edu.my/39609/ http://irep.iium.edu.my/39609/1/dec_2014.pdf http://irep.iium.edu.my/39609/4/39609_Formulation%20and%20stability_Scopus.pdf

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recordtype	eprints
spelling	iium-396092017-09-07T08:25:52Z http://irep.iium.edu.my/39609/ Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application Mohd Shafri, Mohd Affendi Mohamed, Farahidah Yusof, Fatin Athirah Yaacob, Khairul Ikhwan Mohd Yusof, Nazri RS Pharmacy and materia medica An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa (N. sativa) oil in an emulsion to reduce gentamicin toxicity effect over prolonged use in osteo-infection treatment. This work aims to test the aqueous solubility and physicochemical properties of the emulsion. Four emulsions (emulsion A, B, C and D) had been formulated, with final concentration of gentamicin was made constant at 0.1% (w/v) whereas N. sativa oil concentration was varied between 32.5% (v/v) to 46.4% (v/v) in all formulations. Then, stability studies of all emulsion were performed by centrifugation at (5000rpm, 5 minutes), at different storage conditions (8oC, 25oC and 50oC), organoleptic characteristics, freeze-thawcycle, pH determination, particle size measurement, zeta-potential analysis, and pH titration analysis. Results showed no phase separation after centrifugation for freshly prepared emulsions. Storage at 8oC, all emulsions also showed no phase separation at all-time points. At 25oC storage condition, three formulations were stable at day 7 but phase separation was formed in all emulsions by day 14showed good stability at day 7 and all emulsions formed phase separation at day 14. No emulsions were stable in storage temperature of 50oC. The particle size of the emulsions increased with an increment ofN. sativa oil concentration. Zeta-potential analysis showed a range of -32.2 ± 0.15 mV to -48.0 ± 0.45 mV. When pH titration analysis was performed, the zeta potential indicated that the emulsion stability was affected by acidic conditions. We concluded that the use of gentamicin-N. Sativaemulsions must take into account the storage condition with preference of low temperature and fresh preparation at higher alkalinity and the lowest possibility of N. sativa oil. Academic Sciences 2014-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39609/1/dec_2014.pdf application/pdf en http://irep.iium.edu.my/39609/4/39609_Formulation%20and%20stability_Scopus.pdf Mohd Shafri, Mohd Affendi and Mohamed, Farahidah and Yusof, Fatin Athirah and Yaacob, Khairul Ikhwan and Mohd Yusof, Nazri (2014) Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (11). pp. 171-176. ISSN 0975-1491 http://innovareacademics.in/journals/index.php/ijpps/issue/view/Vol6Issue11
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohd Shafri, Mohd Affendi Mohamed, Farahidah Yusof, Fatin Athirah Yaacob, Khairul Ikhwan Mohd Yusof, Nazri Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application
description	An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa (N. sativa) oil in an emulsion to reduce gentamicin toxicity effect over prolonged use in osteo-infection treatment. This work aims to test the aqueous solubility and physicochemical properties of the emulsion. Four emulsions (emulsion A, B, C and D) had been formulated, with final concentration of gentamicin was made constant at 0.1% (w/v) whereas N. sativa oil concentration was varied between 32.5% (v/v) to 46.4% (v/v) in all formulations. Then, stability studies of all emulsion were performed by centrifugation at (5000rpm, 5 minutes), at different storage conditions (8oC, 25oC and 50oC), organoleptic characteristics, freeze-thawcycle, pH determination, particle size measurement, zeta-potential analysis, and pH titration analysis. Results showed no phase separation after centrifugation for freshly prepared emulsions. Storage at 8oC, all emulsions also showed no phase separation at all-time points. At 25oC storage condition, three formulations were stable at day 7 but phase separation was formed in all emulsions by day 14showed good stability at day 7 and all emulsions formed phase separation at day 14. No emulsions were stable in storage temperature of 50oC. The particle size of the emulsions increased with an increment ofN. sativa oil concentration. Zeta-potential analysis showed a range of -32.2 ± 0.15 mV to -48.0 ± 0.45 mV. When pH titration analysis was performed, the zeta potential indicated that the emulsion stability was affected by acidic conditions. We concluded that the use of gentamicin-N. Sativaemulsions must take into account the storage condition with preference of low temperature and fresh preparation at higher alkalinity and the lowest possibility of N. sativa oil.
format	Article
author	Mohd Shafri, Mohd Affendi Mohamed, Farahidah Yusof, Fatin Athirah Yaacob, Khairul Ikhwan Mohd Yusof, Nazri
author_facet	Mohd Shafri, Mohd Affendi Mohamed, Farahidah Yusof, Fatin Athirah Yaacob, Khairul Ikhwan Mohd Yusof, Nazri
author_sort	Mohd Shafri, Mohd Affendi
title	Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application
title_short	Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application
title_full	Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application
title_fullStr	Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application
title_full_unstemmed	Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application
title_sort	formulation and stability testing of gentamicin-n. sativa fusion emulsions for osteo-healing application
publisher	Academic Sciences
publishDate	2014
url	http://irep.iium.edu.my/39609/ http://irep.iium.edu.my/39609/ http://irep.iium.edu.my/39609/1/dec_2014.pdf http://irep.iium.edu.my/39609/4/39609_Formulation%20and%20stability_Scopus.pdf
first_indexed	2023-09-18T20:56:55Z
last_indexed	2023-09-18T20:56:55Z
_version_	1777410366079238144

Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application

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