Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines

Naturally-occurring flavonoids have tremendous potential for producing of new therapeutic agents that provide many benefits to mankind.This study focused on the evaluation of the in vitro cytotoxic, anti-inflammatory and adipocyte differentiation effects of the selected flavonoids which were inophyl...

Full description

Bibliographic Details
Main Authors:	Aminuddin, Amnani, Bakhtiar, M. Taher
Format:	Conference or Workshop Item
Language:	English English English
Published:	2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/39555/ http://irep.iium.edu.my/39555/ http://irep.iium.edu.my/39555/2/IHCI_2014_Abstract_Book.pdf http://irep.iium.edu.my/39555/3/IHCI_2014_Programme_Book.pdf http://irep.iium.edu.my/39555/8/poster_ihci_amnani-2.pdf

id	iium-39555
recordtype	eprints
spelling	iium-395552015-01-06T10:33:46Z http://irep.iium.edu.my/39555/ Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines Aminuddin, Amnani Bakhtiar, M. Taher RS Pharmacy and materia medica Naturally-occurring flavonoids have tremendous potential for producing of new therapeutic agents that provide many benefits to mankind.This study focused on the evaluation of the in vitro cytotoxic, anti-inflammatory and adipocyte differentiation effects of the selected flavonoids which were inophyllum D, calanone, and isocordataoblongic acid from Calophyllumsymingtonianum as well as morelloflavone from Garciniaprainiana on MCF 7 human breast cancer cells, RAW 264.7 macrophages and 3T3-L1 pre-adipocytes respectively. The cytotoxicity study on MCF 7 human breast cancer cells was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation respectively. In the cytotoxicity study, inophyllum D was the only compounds that exhibited significant cytotoxic effect against MCF 7 human breast cancer with IC50 of 84 μg/mL. Further, all the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages with inhibition of nitrite concentration as compared to the positive control. Besides, all the compounds in the range of the tested concentrations also produced adipogenic effects on 3T3-L1 pre-adipocytes and this may suggest that they exhibited potential anti-hyperglycemic property which mimicking the insulin action. Thus, this study may provide significant implication in the discovery of the potential of these selected flavonoids as alternative anti-cancer, anti-inflammatory and anti-hyperglycemic drugs. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/39555/2/IHCI_2014_Abstract_Book.pdf application/pdf en http://irep.iium.edu.my/39555/3/IHCI_2014_Programme_Book.pdf application/pdf en http://irep.iium.edu.my/39555/8/poster_ihci_amnani-2.pdf Aminuddin, Amnani and Bakhtiar, M. Taher (2014) Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines. In: International Health Conference IIUM 2014, 3-4 Dec 2014, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/ihci
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Aminuddin, Amnani Bakhtiar, M. Taher Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
description	Naturally-occurring flavonoids have tremendous potential for producing of new therapeutic agents that provide many benefits to mankind.This study focused on the evaluation of the in vitro cytotoxic, anti-inflammatory and adipocyte differentiation effects of the selected flavonoids which were inophyllum D, calanone, and isocordataoblongic acid from Calophyllumsymingtonianum as well as morelloflavone from Garciniaprainiana on MCF 7 human breast cancer cells, RAW 264.7 macrophages and 3T3-L1 pre-adipocytes respectively. The cytotoxicity study on MCF 7 human breast cancer cells was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation respectively. In the cytotoxicity study, inophyllum D was the only compounds that exhibited significant cytotoxic effect against MCF 7 human breast cancer with IC50 of 84 μg/mL. Further, all the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages with inhibition of nitrite concentration as compared to the positive control. Besides, all the compounds in the range of the tested concentrations also produced adipogenic effects on 3T3-L1 pre-adipocytes and this may suggest that they exhibited potential anti-hyperglycemic property which mimicking the insulin action. Thus, this study may provide significant implication in the discovery of the potential of these selected flavonoids as alternative anti-cancer, anti-inflammatory and anti-hyperglycemic drugs.
format	Conference or Workshop Item
author	Aminuddin, Amnani Bakhtiar, M. Taher
author_facet	Aminuddin, Amnani Bakhtiar, M. Taher
author_sort	Aminuddin, Amnani
title	Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_short	Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_full	Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_fullStr	Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_full_unstemmed	Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_sort	cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
publishDate	2014
url	http://irep.iium.edu.my/39555/ http://irep.iium.edu.my/39555/ http://irep.iium.edu.my/39555/2/IHCI_2014_Abstract_Book.pdf http://irep.iium.edu.my/39555/3/IHCI_2014_Programme_Book.pdf http://irep.iium.edu.my/39555/8/poster_ihci_amnani-2.pdf
first_indexed	2023-09-18T20:56:51Z
last_indexed	2023-09-18T20:56:51Z
_version_	1777410361609158656

Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines

Similar Items