Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis

Plants are important sources of natural product that are widely used as herbal medicine because it is known to be effective with less unwanted side effects. Dissochaetagracilis which also known as ‘Senduduk’ or ‘Cong Keradak was found to be pharmacologically unexplored and the research was conducted...

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Main Authors:	Abd Rahman, Azizah, Bakhtiar, M. Taher
Format:	Conference or Workshop Item
Language:	English English English
Published:	2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/39554/ http://irep.iium.edu.my/39554/ http://irep.iium.edu.my/39554/2/IHCI_2014_Abstract_Book.pdf http://irep.iium.edu.my/39554/3/IHCI_2014_Programme_Book.pdf http://irep.iium.edu.my/39554/8/poster_azizah-3.pdf

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recordtype	eprints
spelling	iium-395542018-06-25T00:50:26Z http://irep.iium.edu.my/39554/ Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis Abd Rahman, Azizah Bakhtiar, M. Taher RS Pharmacy and materia medica Plants are important sources of natural product that are widely used as herbal medicine because it is known to be effective with less unwanted side effects. Dissochaetagracilis which also known as ‘Senduduk’ or ‘Cong Keradak was found to be pharmacologically unexplored and the research was conducted as the thought it is worthwhile to explore it’s potential value. The extraction has been carried out by using soxhlet extraction method with solvent n-Hexane, dichloromethane, and methanol successively. The antimicrobial activities of plant extracts were tested against various microbes which were two Gram-positive bacteria namely Staphylococcus aureus, and Bacillus cereus, two Gram-negative bacteria namely Pseudomonas aeruginosa and Escherichia coli and two fungi consist of Candida albicans and Aspergillus spp. Based on the antimicrobial susceptibility result, the disc diffusion test showed that methanol crude extract of leaves of D. gracilis inhibit the growth of B. cereus, S. aureus, and P.aeruginosa with zone inhibition measured at 13.67±0.58, 12.33±1.53, and 10.33±0.58, respectively. Meanwhile, the n-Hexane, and DCM extracts of D. gracilis showed no antimicrobial activity against all microorganisms tested in this study. The minimum inhibitory concentration (MIC) values of methanol extract for B. cereus, S. aureus, and P. aeruginosawere 6.25, 12.5, and 25.0 mg/mL, respectively. The result obtained from minimum bactericidal concentration (MBC) showed that the methanol extract enable to kill the growth of B.cereus at 12.5 mg/mL, S. aureus at 25 mg/mL and P. aeruginosa at 50 mg/mL. In conclusion, D. gracilis have a potential to be a good antimicrobial agent against some pathogenic strains of microorganisms and thus further investigation need to be done before it can successfully be used to treat certain infectious disease that infect the human. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/39554/2/IHCI_2014_Abstract_Book.pdf application/pdf en http://irep.iium.edu.my/39554/3/IHCI_2014_Programme_Book.pdf application/pdf en http://irep.iium.edu.my/39554/8/poster_azizah-3.pdf Abd Rahman, Azizah and Bakhtiar, M. Taher (2014) Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis. In: International Health Conference IIUM 2014, 3-4 Dec 2014, Kuantan. (Unpublished) http://www.iium.edu.my/ihci
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Abd Rahman, Azizah Bakhtiar, M. Taher Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis
description	Plants are important sources of natural product that are widely used as herbal medicine because it is known to be effective with less unwanted side effects. Dissochaetagracilis which also known as ‘Senduduk’ or ‘Cong Keradak was found to be pharmacologically unexplored and the research was conducted as the thought it is worthwhile to explore it’s potential value. The extraction has been carried out by using soxhlet extraction method with solvent n-Hexane, dichloromethane, and methanol successively. The antimicrobial activities of plant extracts were tested against various microbes which were two Gram-positive bacteria namely Staphylococcus aureus, and Bacillus cereus, two Gram-negative bacteria namely Pseudomonas aeruginosa and Escherichia coli and two fungi consist of Candida albicans and Aspergillus spp. Based on the antimicrobial susceptibility result, the disc diffusion test showed that methanol crude extract of leaves of D. gracilis inhibit the growth of B. cereus, S. aureus, and P.aeruginosa with zone inhibition measured at 13.67±0.58, 12.33±1.53, and 10.33±0.58, respectively. Meanwhile, the n-Hexane, and DCM extracts of D. gracilis showed no antimicrobial activity against all microorganisms tested in this study. The minimum inhibitory concentration (MIC) values of methanol extract for B. cereus, S. aureus, and P. aeruginosawere 6.25, 12.5, and 25.0 mg/mL, respectively. The result obtained from minimum bactericidal concentration (MBC) showed that the methanol extract enable to kill the growth of B.cereus at 12.5 mg/mL, S. aureus at 25 mg/mL and P. aeruginosa at 50 mg/mL. In conclusion, D. gracilis have a potential to be a good antimicrobial agent against some pathogenic strains of microorganisms and thus further investigation need to be done before it can successfully be used to treat certain infectious disease that infect the human.
format	Conference or Workshop Item
author	Abd Rahman, Azizah Bakhtiar, M. Taher
author_facet	Abd Rahman, Azizah Bakhtiar, M. Taher
author_sort	Abd Rahman, Azizah
title	Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis
title_short	Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis
title_full	Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis
title_fullStr	Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis
title_full_unstemmed	Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis
title_sort	antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis
publishDate	2014
url	http://irep.iium.edu.my/39554/ http://irep.iium.edu.my/39554/ http://irep.iium.edu.my/39554/2/IHCI_2014_Abstract_Book.pdf http://irep.iium.edu.my/39554/3/IHCI_2014_Programme_Book.pdf http://irep.iium.edu.my/39554/8/poster_azizah-3.pdf
first_indexed	2023-09-18T20:56:51Z
last_indexed	2023-09-18T20:56:51Z
_version_	1777410361473892352

Antimicrobial and cytotoxic activities of the leaves extracts of dissochaeta gracilis

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