Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves

Infectious diseases and cancer still become a significant health problem that causes high morbidity and mortality rate throughout the world. The resistance and adverse side effects of modern antimicrobial and anticancer drugs were still reported to date. Thus, it is highly required to develop altern...

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Main Authors:	Saflan, Farah Azwani, Bakhtiar, M. Taher, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English English English
Published:	2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/39553/ http://irep.iium.edu.my/39553/ http://irep.iium.edu.my/39553/2/IHCI_2014_Abstract_Book.pdf http://irep.iium.edu.my/39553/3/IHCI_2014_Programme_Book.pdf http://irep.iium.edu.my/39553/8/poster_ICHI_Farah_Azwani-2.pdf

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spelling	iium-395532015-01-06T07:01:09Z http://irep.iium.edu.my/39553/ Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves Saflan, Farah Azwani Bakhtiar, M. Taher Darnis, Deny Susanti RS Pharmacy and materia medica Infectious diseases and cancer still become a significant health problem that causes high morbidity and mortality rate throughout the world. The resistance and adverse side effects of modern antimicrobial and anticancer drugs were still reported to date. Thus, it is highly required to develop alternative therapeutic regimens from natural products of medicinal plants. Medicinal plants have been an important source of healthcare as they contain active substances known as phytochemicals that are useful to treat many diseases. Therefore, the objectives of this study are to investigate the antimicrobial activities of methanol extract of Anisomelesindica’s leaves against Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) and fungi(Candida albicansand Aspergillusspp), as well as cytotoxic activities on human breast adenocarcinoma (MCF 7) and human non-small cell lung carcinoma (H1299) cell lines. The antimicrobial activities were evaluatedusing agar disc-diffusion and determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) / Minimum Fungal Concentration (MFC) values. The cytotoxic activity was carried out using 3-(4,5-dimethylthiazol-2-1)-2,5-diphenyltetrazolium bromide (MTT) assay. The agar disc diffusion test showed that the crude extract inhibited S. aureus (11.3-21.0 mm) and B. cereus (9.7-18.7 mm), while P. aeruginosa, E. coli, C. albicansand Aspergillus spp. were not inhibited for the concentration ranged 50-200 mg/mL tested. The MIC and MBC values for S. aureus are 12.5 and 25 mg/mL, while for B. cereus are 25 and 50 mg/mL, respectively. Cytotoxicity screening results revealed that the plant extract shows no cytotoxic effect against MCF 7 and H1299 cell lines at concentration ranged 6.125-200 μg/mL for 24 hours treatment. In conclusion, screening of methanol extract of A. indica’s leaves shows potential antibacterial activities against S. aureus and B. cereus and no cytotoxic activities against MCF 7 and H1299 cell lines. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/39553/2/IHCI_2014_Abstract_Book.pdf application/pdf en http://irep.iium.edu.my/39553/3/IHCI_2014_Programme_Book.pdf application/pdf en http://irep.iium.edu.my/39553/8/poster_ICHI_Farah_Azwani-2.pdf Saflan, Farah Azwani and Bakhtiar, M. Taher and Darnis, Deny Susanti (2014) Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves. In: International Health Conference IIUM 2014, 3-4 Dec 2014, Kuantan. (Unpublished) http://www.iium.edu.my/ihci
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Saflan, Farah Azwani Bakhtiar, M. Taher Darnis, Deny Susanti Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves
description	Infectious diseases and cancer still become a significant health problem that causes high morbidity and mortality rate throughout the world. The resistance and adverse side effects of modern antimicrobial and anticancer drugs were still reported to date. Thus, it is highly required to develop alternative therapeutic regimens from natural products of medicinal plants. Medicinal plants have been an important source of healthcare as they contain active substances known as phytochemicals that are useful to treat many diseases. Therefore, the objectives of this study are to investigate the antimicrobial activities of methanol extract of Anisomelesindica’s leaves against Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) and fungi(Candida albicansand Aspergillusspp), as well as cytotoxic activities on human breast adenocarcinoma (MCF 7) and human non-small cell lung carcinoma (H1299) cell lines. The antimicrobial activities were evaluatedusing agar disc-diffusion and determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) / Minimum Fungal Concentration (MFC) values. The cytotoxic activity was carried out using 3-(4,5-dimethylthiazol-2-1)-2,5-diphenyltetrazolium bromide (MTT) assay. The agar disc diffusion test showed that the crude extract inhibited S. aureus (11.3-21.0 mm) and B. cereus (9.7-18.7 mm), while P. aeruginosa, E. coli, C. albicansand Aspergillus spp. were not inhibited for the concentration ranged 50-200 mg/mL tested. The MIC and MBC values for S. aureus are 12.5 and 25 mg/mL, while for B. cereus are 25 and 50 mg/mL, respectively. Cytotoxicity screening results revealed that the plant extract shows no cytotoxic effect against MCF 7 and H1299 cell lines at concentration ranged 6.125-200 μg/mL for 24 hours treatment. In conclusion, screening of methanol extract of A. indica’s leaves shows potential antibacterial activities against S. aureus and B. cereus and no cytotoxic activities against MCF 7 and H1299 cell lines.
format	Conference or Workshop Item
author	Saflan, Farah Azwani Bakhtiar, M. Taher Darnis, Deny Susanti
author_facet	Saflan, Farah Azwani Bakhtiar, M. Taher Darnis, Deny Susanti
author_sort	Saflan, Farah Azwani
title	Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves
title_short	Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves
title_full	Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves
title_fullStr	Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves
title_full_unstemmed	Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves
title_sort	antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves
publishDate	2014
url	http://irep.iium.edu.my/39553/ http://irep.iium.edu.my/39553/ http://irep.iium.edu.my/39553/2/IHCI_2014_Abstract_Book.pdf http://irep.iium.edu.my/39553/3/IHCI_2014_Programme_Book.pdf http://irep.iium.edu.my/39553/8/poster_ICHI_Farah_Azwani-2.pdf
first_indexed	2023-09-18T20:56:51Z
last_indexed	2023-09-18T20:56:51Z
_version_	1777410361320800256

Antimicrobial and cytotoxic activities of methanol extract of anisomeles indica leaves

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