Antimicrobial and cytotoxicity activities of sterculia parviflora

In this modern world, plants and natural resources are being so significant in various pharmacological and drug research. The search for new antimicrobial and anticancer remedies is among the most prominent research fields nowadays. The aims of the present study are to evaluate the antimicrobial and...

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Main Authors:	Rozmi, Muhammad Syamil, Bakhtiar, M. Taher, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English English English
Published:	2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/39551/ http://irep.iium.edu.my/39551/ http://irep.iium.edu.my/39551/2/IHCI_2014_Abstract_Book.pdf http://irep.iium.edu.my/39551/3/IHCI_2014_Programme_Book.pdf http://irep.iium.edu.my/39551/8/Poster_Presentation_zoey.pdf

id	iium-39551
recordtype	eprints
spelling	iium-395512018-05-24T04:08:50Z http://irep.iium.edu.my/39551/ Antimicrobial and cytotoxicity activities of sterculia parviflora Rozmi, Muhammad Syamil Bakhtiar, M. Taher Darnis, Deny Susanti RS Pharmacy and materia medica In this modern world, plants and natural resources are being so significant in various pharmacological and drug research. The search for new antimicrobial and anticancer remedies is among the most prominent research fields nowadays. The aims of the present study are to evaluate the antimicrobial and cytotoxic activity of Sterculiaparviflora against the selected microorganisms and breast cancer cell line (MCF-7) respectively. S. parviflora’s leaves were extracted with n-hexane, ethyl acetate and methanol by using Soxhlet apparatus. At first, the extracts were analyzed for their phytochemical constituents such as alkaloids, flavonoids, saponins, steroids, terpenoids and phenolic. In the antimicrobial screening, the crude extracts were evaluated through disc diffusion and micro dilution methods against two Gram-positive bacteria (S. aureus and B. cereus), two Gram-negative bacteria (P. aeruginosa and E. coli) and two fungi (C. albicanand Aspergillus spp.). In disc diffusion method, the methanol extract exhibited antimicrobial potency against S. aureus and B. cereus which ranged within 8 to 10.7 mm of inhibition zone while ethyl acetate only inhibited B. cereus ranged within 14.7 to 26.3 mm. The Minimum Inhibitory Concentration (MIC) values of methanol extract against both S. aureus and B. cereus is 25 mg/mL while 50 mg/mL against B. cereus for ethyl acetate. The Minimum Bactericidal Concentration (MBC) values which indicated completely inhibition without visible growth of bacteria is at concentration of 50 mg/mL for methanol extract against both S. aureus and B. cereus while 100 mg/mL for ethyl acetate extract against B. cereus. In cytotoxicity study, MTT assay and tryphan blue exclusion methods were done to assess the percentage of viable cells of MCF-7 cell line after being treated with S. parviflora extracts. The results showed that all extracts did not exhibit significant cytotoxic effect on MCF-7 cancer cell line at concentrations of 100, 50, 25, 12.5, 6.25 and 3.125 mg/mL after 24 hours incubation. Moreover, the IC50 values of each extracts also would not be determined due to inability of extracts to reduce the viable cells percentage to be lower than 50%. In conclusion, the S. parviflora extracts exhibited potential antimicrobial activity against S. aureus and B. cereus while possessed no cytotoxic potency against MCF-7 breast cancer cell line. 2014-12 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/39551/2/IHCI_2014_Abstract_Book.pdf application/pdf en http://irep.iium.edu.my/39551/3/IHCI_2014_Programme_Book.pdf application/pdf en http://irep.iium.edu.my/39551/8/Poster_Presentation_zoey.pdf Rozmi, Muhammad Syamil and Bakhtiar, M. Taher and Darnis, Deny Susanti (2014) Antimicrobial and cytotoxicity activities of sterculia parviflora. In: International Health Conference IIUM 2014, 3-4 Dec 2014, Kuantan. (Unpublished) http://www.iium.edu.my/ihci
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Rozmi, Muhammad Syamil Bakhtiar, M. Taher Darnis, Deny Susanti Antimicrobial and cytotoxicity activities of sterculia parviflora
description	In this modern world, plants and natural resources are being so significant in various pharmacological and drug research. The search for new antimicrobial and anticancer remedies is among the most prominent research fields nowadays. The aims of the present study are to evaluate the antimicrobial and cytotoxic activity of Sterculiaparviflora against the selected microorganisms and breast cancer cell line (MCF-7) respectively. S. parviflora’s leaves were extracted with n-hexane, ethyl acetate and methanol by using Soxhlet apparatus. At first, the extracts were analyzed for their phytochemical constituents such as alkaloids, flavonoids, saponins, steroids, terpenoids and phenolic. In the antimicrobial screening, the crude extracts were evaluated through disc diffusion and micro dilution methods against two Gram-positive bacteria (S. aureus and B. cereus), two Gram-negative bacteria (P. aeruginosa and E. coli) and two fungi (C. albicanand Aspergillus spp.). In disc diffusion method, the methanol extract exhibited antimicrobial potency against S. aureus and B. cereus which ranged within 8 to 10.7 mm of inhibition zone while ethyl acetate only inhibited B. cereus ranged within 14.7 to 26.3 mm. The Minimum Inhibitory Concentration (MIC) values of methanol extract against both S. aureus and B. cereus is 25 mg/mL while 50 mg/mL against B. cereus for ethyl acetate. The Minimum Bactericidal Concentration (MBC) values which indicated completely inhibition without visible growth of bacteria is at concentration of 50 mg/mL for methanol extract against both S. aureus and B. cereus while 100 mg/mL for ethyl acetate extract against B. cereus. In cytotoxicity study, MTT assay and tryphan blue exclusion methods were done to assess the percentage of viable cells of MCF-7 cell line after being treated with S. parviflora extracts. The results showed that all extracts did not exhibit significant cytotoxic effect on MCF-7 cancer cell line at concentrations of 100, 50, 25, 12.5, 6.25 and 3.125 mg/mL after 24 hours incubation. Moreover, the IC50 values of each extracts also would not be determined due to inability of extracts to reduce the viable cells percentage to be lower than 50%. In conclusion, the S. parviflora extracts exhibited potential antimicrobial activity against S. aureus and B. cereus while possessed no cytotoxic potency against MCF-7 breast cancer cell line.
format	Conference or Workshop Item
author	Rozmi, Muhammad Syamil Bakhtiar, M. Taher Darnis, Deny Susanti
author_facet	Rozmi, Muhammad Syamil Bakhtiar, M. Taher Darnis, Deny Susanti
author_sort	Rozmi, Muhammad Syamil
title	Antimicrobial and cytotoxicity activities of sterculia parviflora
title_short	Antimicrobial and cytotoxicity activities of sterculia parviflora
title_full	Antimicrobial and cytotoxicity activities of sterculia parviflora
title_fullStr	Antimicrobial and cytotoxicity activities of sterculia parviflora
title_full_unstemmed	Antimicrobial and cytotoxicity activities of sterculia parviflora
title_sort	antimicrobial and cytotoxicity activities of sterculia parviflora
publishDate	2014
url	http://irep.iium.edu.my/39551/ http://irep.iium.edu.my/39551/ http://irep.iium.edu.my/39551/2/IHCI_2014_Abstract_Book.pdf http://irep.iium.edu.my/39551/3/IHCI_2014_Programme_Book.pdf http://irep.iium.edu.my/39551/8/Poster_Presentation_zoey.pdf
first_indexed	2023-09-18T20:56:51Z
last_indexed	2023-09-18T20:56:51Z
_version_	1777410361041879040

Antimicrobial and cytotoxicity activities of sterculia parviflora

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