The growth inhibitory effect of Henna (L.inermis) Aqueous extract in human lung cancer cells, A549
Chemotherapy is the common treatment used to handle cancer cases. Since the drugs used in chemotherapy act on human body by damaging high proliferating cells, normal cell for instance bone marrow, hair follicles and gastrointestinal mucosa which undergoes high rates of cell division are most susc...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English English English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/39526/ http://irep.iium.edu.my/39526/ http://irep.iium.edu.my/39526/1/IHCI_2014_Programme_Book.pdf http://irep.iium.edu.my/39526/3/POSTER_IHCI_2014.pdf http://irep.iium.edu.my/39526/2/IHCI_2014_Abstract_Book.pdf |
| Summary: | Chemotherapy is the common treatment used to handle cancer cases. Since the drugs used in
chemotherapy act on human body by damaging high proliferating cells, normal cell for
instance bone marrow, hair follicles and gastrointestinal mucosa which undergoes high rates
of cell division are most susceptible. It also produces common side effects like neutropenia,
anorexia, diarrhea and many others. In recent study, Lawsoniainermisor henna can become
one of the potential natural therapies to treat common cancer cells (Pradhan et al., 2012).
Many studies had explored the properties of henna for its scientific components by extracting
certain compound but did not associate the usage of all components of henna in one study.
This study was carried out with the aims to observe the pattern of A549 cells growth before
and after being treated with henna and to determine the inhibitory effects of boiled henna
liquid in human lung adenocarcinoma epithelial cells (A549). To compare the A549 cell
growth before and after the addition of henna, cell number and protein content of the culture
were analyzed. Boiled liquid of henna was used so that all of the components would be
extracted in the liquid. MTT assay, trypan blue exclusion method and Lowry assay were
employed to identify the inhibitory effects of boiled henna, to obtain the standard growth
curve and to assess the protein content respectively. The result revealed that henna has the
ability to inhibit the growth of A549 cells by showing an IC50 value of 5% after 24 hours
exposure and 2% after 48 and 72 hours exposure. The growth curve obtained showed a
significant difference in inhibition of A549 cell growth after been exposed to henna
compared to untreated A549 (p<0.05). The protein content had a moderate correlation with
cell number after being treated with boiled henna (r2= 0.4966). In conclusion, the findings
revealed that henna has cytotoxic effects against A549 cell lines. This basic study will help in
providing scientific justification for the use of this prophetic medicine in treating cancer.
Keywords:Lawsoniainermis, henna, inhibitory effects, MTT assay,A549 |
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