Extraction and purification of antifungal compounds from piper betle

Piper betle also known as ?sireh? has been traditionally used for medicinal purposes. It has been reported that P. betle leaves possess antifungal activity but no effort to optimize the extraction conditions to obtain high yield of antifungal compounds has been carried out yet. In this study, optimi...

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Main Authors:	Ahmad Raus, Raha, Surmi, Amira, Junoh, Hafizan, Jamal, Parveen
Format:	Article
Language:	English English
Published:	Journal of Pure and Applied Microbiology 2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/39367/ http://irep.iium.edu.my/39367/ http://irep.iium.edu.my/39367/1/Extraction_Antifungal_from_Pbetle.pdf http://irep.iium.edu.my/39367/3/39367_Extraction%20and%20purification_Scopus.pdf

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recordtype	eprints
spelling	iium-393672017-09-07T02:50:16Z http://irep.iium.edu.my/39367/ Extraction and purification of antifungal compounds from piper betle Ahmad Raus, Raha Surmi, Amira Junoh, Hafizan Jamal, Parveen Q Science (General) Piper betle also known as ?sireh? has been traditionally used for medicinal purposes. It has been reported that P. betle leaves possess antifungal activity but no effort to optimize the extraction conditions to obtain high yield of antifungal compounds has been carried out yet. In this study, optimized extraction condition was determined. It was first conducted by screening parameters that significantly affect the extraction using Fractional Factorial Analysis. This is followed by identifying the levels for those parameters by employing under Response Surface Method (RSM) FFA. Linear effects of temperature and solvent to solvent ratio as well as interactive terms between temperature and incubation time, and between temperature and solvent to solvent ratio were found to significantly (p<0.05) affect the extraction. Temperature and incubation time were selected for optimization experiment and the best condition obtained was extraction for 15 hours at 50oC. In the present study, purification of the extracted antifungal compounds was also carried out. Using column chromatography, the resulting fractions from elution with mixture of hexane and ethyl acetate at ratio 8:2 successfully purified the antifungal compounds. Thin layer chromatography (TLC) showed two distinct spots at Rf value 0.82 and 0.86. Identity of the two spots will be carried out in future research. Journal of Pure and Applied Microbiology 2014-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39367/1/Extraction_Antifungal_from_Pbetle.pdf application/pdf en http://irep.iium.edu.my/39367/3/39367_Extraction%20and%20purification_Scopus.pdf Ahmad Raus, Raha and Surmi, Amira and Junoh, Hafizan and Jamal, Parveen (2014) Extraction and purification of antifungal compounds from piper betle. Journal of Pure and Applied Microbiology, 8 Spl. pp. 839-844. ISSN 0973-7510 http://www.microbiologyjournal.org/jmabsarchive.php?vol=8&issue=May%202014%20Special%20Edition
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Ahmad Raus, Raha Surmi, Amira Junoh, Hafizan Jamal, Parveen Extraction and purification of antifungal compounds from piper betle
description	Piper betle also known as ?sireh? has been traditionally used for medicinal purposes. It has been reported that P. betle leaves possess antifungal activity but no effort to optimize the extraction conditions to obtain high yield of antifungal compounds has been carried out yet. In this study, optimized extraction condition was determined. It was first conducted by screening parameters that significantly affect the extraction using Fractional Factorial Analysis. This is followed by identifying the levels for those parameters by employing under Response Surface Method (RSM) FFA. Linear effects of temperature and solvent to solvent ratio as well as interactive terms between temperature and incubation time, and between temperature and solvent to solvent ratio were found to significantly (p<0.05) affect the extraction. Temperature and incubation time were selected for optimization experiment and the best condition obtained was extraction for 15 hours at 50oC. In the present study, purification of the extracted antifungal compounds was also carried out. Using column chromatography, the resulting fractions from elution with mixture of hexane and ethyl acetate at ratio 8:2 successfully purified the antifungal compounds. Thin layer chromatography (TLC) showed two distinct spots at Rf value 0.82 and 0.86. Identity of the two spots will be carried out in future research.
format	Article
author	Ahmad Raus, Raha Surmi, Amira Junoh, Hafizan Jamal, Parveen
author_facet	Ahmad Raus, Raha Surmi, Amira Junoh, Hafizan Jamal, Parveen
author_sort	Ahmad Raus, Raha
title	Extraction and purification of antifungal compounds from piper betle
title_short	Extraction and purification of antifungal compounds from piper betle
title_full	Extraction and purification of antifungal compounds from piper betle
title_fullStr	Extraction and purification of antifungal compounds from piper betle
title_full_unstemmed	Extraction and purification of antifungal compounds from piper betle
title_sort	extraction and purification of antifungal compounds from piper betle
publisher	Journal of Pure and Applied Microbiology
publishDate	2014
url	http://irep.iium.edu.my/39367/ http://irep.iium.edu.my/39367/ http://irep.iium.edu.my/39367/1/Extraction_Antifungal_from_Pbetle.pdf http://irep.iium.edu.my/39367/3/39367_Extraction%20and%20purification_Scopus.pdf
first_indexed	2023-09-18T20:56:34Z
last_indexed	2023-09-18T20:56:34Z
_version_	1777410343208747008

Extraction and purification of antifungal compounds from piper betle

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