Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera

Cross linked enzyme aggregates technology is one of the latest methods in immobilization of enzymes. This technique provides biocatalysts with higher activity, stability and reusability in industrial applications. In this research, lipase was extracted from the viscera of river channel catfish (Ictu...

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Main Authors:	Mahmod, Safa Senan, Yusof, Faridah, Jami, Mohammed Saedi
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/39224/ http://irep.iium.edu.my/39224/ http://irep.iium.edu.my/39224/1/39224.pdf

id	iium-39224
recordtype	eprints
spelling	iium-392242015-04-10T01:45:26Z http://irep.iium.edu.my/39224/ Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera Mahmod, Safa Senan Yusof, Faridah Jami, Mohammed Saedi TP248.13 Biotechnology Cross linked enzyme aggregates technology is one of the latest methods in immobilization of enzymes. This technique provides biocatalysts with higher activity, stability and reusability in industrial applications. In this research, lipase was extracted from the viscera of river channel catfish (Ictularus punctatus) and was immobilized based on cross-liked enzyme aggregate methodology. Glutaraldehyde was used as a cross-linker, acetone as precipitant and bovine serum albumin as an additive to improve the formation of CLEA-lipase. Experimental design was carried out by Response Surface Methodology using central composite design to find the optimum conditions for immobilization which was 50 mM of glutaraldehyde, 60% (v/v) Acetone and 0.11 mM of BSA. Finally, CLEA-lipase’s specific activity was 1.713 U/mg that is 20.43% higher than the free lipase activity. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/39224/1/39224.pdf Mahmod, Safa Senan and Yusof, Faridah and Jami, Mohammed Saedi (2014) Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera. In: International Conference on Biological, Chemical and Environmental Sciences (BCES-2014), 14-15 Jun 2014, Penang. http://iicbe.org/siteadmin/upload/7773C614017.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mahmod, Safa Senan Yusof, Faridah Jami, Mohammed Saedi Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
description	Cross linked enzyme aggregates technology is one of the latest methods in immobilization of enzymes. This technique provides biocatalysts with higher activity, stability and reusability in industrial applications. In this research, lipase was extracted from the viscera of river channel catfish (Ictularus punctatus) and was immobilized based on cross-liked enzyme aggregate methodology. Glutaraldehyde was used as a cross-linker, acetone as precipitant and bovine serum albumin as an additive to improve the formation of CLEA-lipase. Experimental design was carried out by Response Surface Methodology using central composite design to find the optimum conditions for immobilization which was 50 mM of glutaraldehyde, 60% (v/v) Acetone and 0.11 mM of BSA. Finally, CLEA-lipase’s specific activity was 1.713 U/mg that is 20.43% higher than the free lipase activity.
format	Conference or Workshop Item
author	Mahmod, Safa Senan Yusof, Faridah Jami, Mohammed Saedi
author_facet	Mahmod, Safa Senan Yusof, Faridah Jami, Mohammed Saedi
author_sort	Mahmod, Safa Senan
title	Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_short	Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_full	Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_fullStr	Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_full_unstemmed	Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_sort	production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
publishDate	2014
url	http://irep.iium.edu.my/39224/ http://irep.iium.edu.my/39224/ http://irep.iium.edu.my/39224/1/39224.pdf
first_indexed	2023-09-18T20:56:20Z
last_indexed	2023-09-18T20:56:20Z
_version_	1777410328593694720

Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera

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