Production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors

The present work was aimed towards the evaluation of effects of the physical and chemical factors on laccase production by white rot fungus, Resinicium bicolor (ATCC 64897) under submerged condition in shake flask using Plackett-Burman design. R.bicolor potentially detoxifies ground tire rubber (GTR...

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Main Authors: Shah, Harmen, Yusof, Faridah, Alam, Md Zahangir
Format: Article
Language:English
Published: Seek Digital Library 2014
Subjects:
Online Access:http://irep.iium.edu.my/39222/
http://irep.iium.edu.my/39222/
http://irep.iium.edu.my/39222/1/P_47.pdf
id iium-39222
recordtype eprints
spelling iium-392222017-08-10T07:41:07Z http://irep.iium.edu.my/39222/ Production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors Shah, Harmen Yusof, Faridah Alam, Md Zahangir TP248.13 Biotechnology The present work was aimed towards the evaluation of effects of the physical and chemical factors on laccase production by white rot fungus, Resinicium bicolor (ATCC 64897) under submerged condition in shake flask using Plackett-Burman design. R.bicolor potentially detoxifies ground tire rubber (GTR) and facilitates growth of Thiobacillus ferrooxidans, a bacterium that performs devulcanization of GTR but is sensitive towards rubber additives present in GTR. In this experiment out of 11 factors screened, copper sulphate and yellow flame bark powder were found to be the most significant factors affecting the laccase production. The study indicated that the screened factors can be optimized for enhanced laccase production and can potentially be utilized for attaining maximum detoxification of GTR and hence further devulcanization. Seek Digital Library 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39222/1/P_47.pdf Shah, Harmen and Yusof, Faridah and Alam, Md Zahangir (2014) Production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors. International Journal of Environmental Engineering, 1 (2). pp. 146-150. ISSN 1756-8463 E-ISSN 1756-8471 http://www.seekdl.org/nm.php?id=3211
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Shah, Harmen
Yusof, Faridah
Alam, Md Zahangir
Production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors
description The present work was aimed towards the evaluation of effects of the physical and chemical factors on laccase production by white rot fungus, Resinicium bicolor (ATCC 64897) under submerged condition in shake flask using Plackett-Burman design. R.bicolor potentially detoxifies ground tire rubber (GTR) and facilitates growth of Thiobacillus ferrooxidans, a bacterium that performs devulcanization of GTR but is sensitive towards rubber additives present in GTR. In this experiment out of 11 factors screened, copper sulphate and yellow flame bark powder were found to be the most significant factors affecting the laccase production. The study indicated that the screened factors can be optimized for enhanced laccase production and can potentially be utilized for attaining maximum detoxification of GTR and hence further devulcanization.
format Article
author Shah, Harmen
Yusof, Faridah
Alam, Md Zahangir
author_facet Shah, Harmen
Yusof, Faridah
Alam, Md Zahangir
author_sort Shah, Harmen
title Production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors
title_short Production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors
title_full Production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors
title_fullStr Production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors
title_full_unstemmed Production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors
title_sort production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors
publisher Seek Digital Library
publishDate 2014
url http://irep.iium.edu.my/39222/
http://irep.iium.edu.my/39222/
http://irep.iium.edu.my/39222/1/P_47.pdf
first_indexed 2023-09-18T20:56:19Z
last_indexed 2023-09-18T20:56:19Z
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