Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius

A solvent screening study using four alcohols with decreasing polarity to select novel acidified extraction solvents for maximal antimicrobial peptide (AMPs) recovery from the whole body extracts of supermealworm Zophobas morio larvae was performed. 20 grams of final instar larvae were homogenized i...

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Main Authors:	Mohta, Johan Ariff, Yusof, Faridah, Ali, Najala Mahmoud Hag
Format:	Article
Language:	English
Published:	AENSI Publications 2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/39220/ http://irep.iium.edu.my/39220/1/P_45.pdf

id	iium-39220
recordtype	eprints
spelling	iium-392202017-06-13T07:23:16Z http://irep.iium.edu.my/39220/ Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius Mohta, Johan Ariff Yusof, Faridah Ali, Najala Mahmoud Hag TP248.13 Biotechnology A solvent screening study using four alcohols with decreasing polarity to select novel acidified extraction solvents for maximal antimicrobial peptide (AMPs) recovery from the whole body extracts of supermealworm Zophobas morio larvae was performed. 20 grams of final instar larvae were homogenized in four solvents at fixed concentration of 90% methanol, 90% ethanol, 90% isopropanol and 90% butanol; each acidified with trifluoroacetic acid (TFA). All samples were extracted at constant solid-solvent ratio (w/v), extraction time and temperature. The solvent extracts were tested on four bacteria in the antimicrobial susceptibility test to evaluate the inhibitory effects. Inhibition zones (mm) were recorded and the range of values was categorized as good, moderate, weak and very weak antibacterial activity. All extracts were found to exhibit significant inhibitory effects on the bacteria (p < 0.001). Isopropanol extract produced significantly (p < 0.05) the largest zone of inhibition in Staphylococcus aureus (10.67 ± 3.06 mm), Escherichia coli (8.67 ± 2.08 mm), Klebsiella pneumonia (8.00 ± 1.00 mm) and Pseudomonas aeroginosa (7.00 ± 2.65 mm) compared to other solvent extracts. The extract was considered a good antibacterial agent against S. aureus, E. coli, and K. pneumonia and as a moderate antibacterial agent against P. aeroginosa. S. aureus was shown to be the most susceptible to isopropanol extract followed by E. coli, K. pneumonia and P. aeroginosa. This finding has a significant implication to improve the extraction procedure over the use of conventional methanolic extraction for new AMP discovery from insect whole body extracts. It appears that acidified isopropanol extracted a maximum amount of AMPs compared to acidified methanol, hence, is selected as the best novel solvent in this study. AENSI Publications 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39220/1/P_45.pdf Mohta, Johan Ariff and Yusof, Faridah and Ali, Najala Mahmoud Hag (2014) Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius. Advances in Environmental Biology, 8 (3). pp. 803-809. ISSN 1995-0756 E-ISSN 1998-1066
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohta, Johan Ariff Yusof, Faridah Ali, Najala Mahmoud Hag Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
description	A solvent screening study using four alcohols with decreasing polarity to select novel acidified extraction solvents for maximal antimicrobial peptide (AMPs) recovery from the whole body extracts of supermealworm Zophobas morio larvae was performed. 20 grams of final instar larvae were homogenized in four solvents at fixed concentration of 90% methanol, 90% ethanol, 90% isopropanol and 90% butanol; each acidified with trifluoroacetic acid (TFA). All samples were extracted at constant solid-solvent ratio (w/v), extraction time and temperature. The solvent extracts were tested on four bacteria in the antimicrobial susceptibility test to evaluate the inhibitory effects. Inhibition zones (mm) were recorded and the range of values was categorized as good, moderate, weak and very weak antibacterial activity. All extracts were found to exhibit significant inhibitory effects on the bacteria (p < 0.001). Isopropanol extract produced significantly (p < 0.05) the largest zone of inhibition in Staphylococcus aureus (10.67 ± 3.06 mm), Escherichia coli (8.67 ± 2.08 mm), Klebsiella pneumonia (8.00 ± 1.00 mm) and Pseudomonas aeroginosa (7.00 ± 2.65 mm) compared to other solvent extracts. The extract was considered a good antibacterial agent against S. aureus, E. coli, and K. pneumonia and as a moderate antibacterial agent against P. aeroginosa. S. aureus was shown to be the most susceptible to isopropanol extract followed by E. coli, K. pneumonia and P. aeroginosa. This finding has a significant implication to improve the extraction procedure over the use of conventional methanolic extraction for new AMP discovery from insect whole body extracts. It appears that acidified isopropanol extracted a maximum amount of AMPs compared to acidified methanol, hence, is selected as the best novel solvent in this study.
format	Article
author	Mohta, Johan Ariff Yusof, Faridah Ali, Najala Mahmoud Hag
author_facet	Mohta, Johan Ariff Yusof, Faridah Ali, Najala Mahmoud Hag
author_sort	Mohta, Johan Ariff
title	Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_short	Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_full	Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_fullStr	Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_full_unstemmed	Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_sort	screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
publisher	AENSI Publications
publishDate	2014
url	http://irep.iium.edu.my/39220/ http://irep.iium.edu.my/39220/1/P_45.pdf
first_indexed	2023-09-18T20:56:19Z
last_indexed	2023-09-18T20:56:19Z
_version_	1777410328007540736

Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius

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