Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken

Bacteriocins produced by a variety of lactic acid bacteria (LAB) are gaining more attention for not only as alternative food preservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously...

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Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Alhaji Yusuf, Moshood, Arief Ichwan, Solachuddin Jauhari
Format:	Article
Language:	English
Published:	OMICS Group 2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/39055/ http://irep.iium.edu.my/39055/ http://irep.iium.edu.my/39055/ http://irep.iium.edu.my/39055/1/haziyamin.pdf

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recordtype	eprints
spelling	iium-390552018-06-18T15:18:47Z http://irep.iium.edu.my/39055/ Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari Q Science (General) Bacteriocins produced by a variety of lactic acid bacteria (LAB) are gaining more attention for not only as alternative food preservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously isolated probiotic from Malaysian non-broiler chicken. Based on agar diffusion assay, it showed antimicrobial activities against Methicillin resistance Staphylococcus aureus (MRSA) used as an indicator organism. Approximately 10 kDa protein was purified employing three-phase partitioning (TPP) method and it was shown to be highly thermostable, retaining activities at 121°C for 15min, and was stable in a pH range of 4-9. There was however a loss in activity after protease treatment. PCR amplification using enterocin gene primers showed that Ent C4L10 sequence is highly similar to Bacteriocin L-1077 (83% identity). In order to study its anti-proliferative potential, purified Ent C4L10 was also tested against four human cell lines; i.e., lung cancer (H1299), breast cancer (MCF 7), colon cancer (HCT116) and oral cancer (HSC3). It was found that oral cancer cell line was the most sensitive with a cytotoxic index of IC50 of 9.009µg/ml, followed by breast cancer (IC50 (11.51µg/ml), and the least sensitive was with colon cancer cell line (IC50 of 20.57µg/ml). In conclusion, putative 10 KDa Ent C4L10 is a class IIa bacteriocin isolated from coagulase negative Enterococcus mundtii strain C4L10 shown to have anti-proliferative properties. Therefore, this bacteriocin has not only great potential for use in food preservation, it future use as an antitumor agent should also be explored OMICS Group 2014-09-23 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39055/1/haziyamin.pdf Tengku Abdul Hamid, Tengku Haziyamin and Alhaji Yusuf, Moshood and Arief Ichwan, Solachuddin Jauhari (2014) Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken. Journal of Probiotics & Health, 2 (2). p. 53. ISSN 2329-8901 http://omicsonline.org/2329-8901/2329-8901.S1.015-015.pdf 10.4172/2329-8901.S1.015
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken
description	Bacteriocins produced by a variety of lactic acid bacteria (LAB) are gaining more attention for not only as alternative food preservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously isolated probiotic from Malaysian non-broiler chicken. Based on agar diffusion assay, it showed antimicrobial activities against Methicillin resistance Staphylococcus aureus (MRSA) used as an indicator organism. Approximately 10 kDa protein was purified employing three-phase partitioning (TPP) method and it was shown to be highly thermostable, retaining activities at 121°C for 15min, and was stable in a pH range of 4-9. There was however a loss in activity after protease treatment. PCR amplification using enterocin gene primers showed that Ent C4L10 sequence is highly similar to Bacteriocin L-1077 (83% identity). In order to study its anti-proliferative potential, purified Ent C4L10 was also tested against four human cell lines; i.e., lung cancer (H1299), breast cancer (MCF 7), colon cancer (HCT116) and oral cancer (HSC3). It was found that oral cancer cell line was the most sensitive with a cytotoxic index of IC50 of 9.009µg/ml, followed by breast cancer (IC50 (11.51µg/ml), and the least sensitive was with colon cancer cell line (IC50 of 20.57µg/ml). In conclusion, putative 10 KDa Ent C4L10 is a class IIa bacteriocin isolated from coagulase negative Enterococcus mundtii strain C4L10 shown to have anti-proliferative properties. Therefore, this bacteriocin has not only great potential for use in food preservation, it future use as an antitumor agent should also be explored
format	Article
author	Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari
author_facet	Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari
author_sort	Tengku Abdul Hamid, Tengku Haziyamin
title	Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken
title_short	Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken
title_full	Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken
title_fullStr	Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken
title_full_unstemmed	Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken
title_sort	anti-proliferative bacteriocins active against mrsa from coagulase negative ent. mundtii strain c4l10 isolated from non-broiler chicken
publisher	OMICS Group
publishDate	2014
url	http://irep.iium.edu.my/39055/ http://irep.iium.edu.my/39055/ http://irep.iium.edu.my/39055/ http://irep.iium.edu.my/39055/1/haziyamin.pdf
first_indexed	2023-09-18T20:56:05Z
last_indexed	2023-09-18T20:56:05Z
_version_	1777410313764732928

Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken

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