Biotransformation of ω fatty acids using pseudomonas aeruginosa
Biotransformation of fish oil containing ω fatty acids using Pseudomonas spp has been carried out. In the present study, Pseudomonas aeruginosa was used to transform ω fatty acids, extracted from Malaysian catfish Clarias batrachus, into useful metabolite. The fatty acids were extracted from the f...
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| Format: | Conference or Workshop Item |
| Language: | English English English English |
| Published: |
2012
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| Online Access: | http://irep.iium.edu.my/39021/ http://irep.iium.edu.my/39021/1/IBS_2012_Poster.pdf http://irep.iium.edu.my/39021/3/IBS__2012_Poster_Biotransformation_of_%CF%89_fatty_acids_using_Pseudomonas_aeruginosa.pdf http://irep.iium.edu.my/39021/2/DR_NO_Abstract_IBS_2012.pdf http://irep.iium.edu.my/39021/9/IBS2012-2.pdf |
| Summary: | Biotransformation of fish oil containing ω fatty acids using Pseudomonas spp has been carried out. In the
present study, Pseudomonas aeruginosa was used to transform ω fatty acids, extracted from Malaysian
catfish Clarias batrachus, into useful metabolite. The fatty acids were extracted from the freeze-dried
catfish flesh using a modified Folch method with the chloroform and methanol system. The crude oil was
added to the bacterial culture and incubated at 28oC for 4 days. After incubation, the biotransformed
product was extracted and analyzed by using gas chromatography and mass spectrometer (GC-MS) to
identify the fatty acids and other compounds. It was found that several fatty acids, especially ω-fatty acids
were converted to hydroxyl fatty acids. This indicates that ω-fatty acids can be used as starting materials
for other bioactive metabolites for pharmaceutical purposes. |
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