Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain

Bromelain, a naturally available therapeutic protease enzyme from pineapple stem, was expressed in Escherichia coli using autoinduction-based fermentation medium. There are numerous recombinant proteins functionally expressed in E. coli using this autoinduction approach. Preliminary media trials...

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Main Authors:	Jamaluddin, Mohd Jamil Aizat, Amid, Azura, Azmi, Azlin Suhaida, Othman, Muhd. Ezza Faiez
Format:	Article
Language:	English English
Published:	Journal of Pure and Applied Microbiology 2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/38448/ http://irep.iium.edu.my/38448/ http://irep.iium.edu.my/38448/1/May_Jamil_JPAM.pdf http://irep.iium.edu.my/38448/3/38448_Screening%20of%20important_scopus.pdf

id	iium-38448
recordtype	eprints
spelling	iium-384482017-09-25T10:41:44Z http://irep.iium.edu.my/38448/ Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain Jamaluddin, Mohd Jamil Aizat Amid, Azura Azmi, Azlin Suhaida Othman, Muhd. Ezza Faiez TP248.13 Biotechnology Bromelain, a naturally available therapeutic protease enzyme from pineapple stem, was expressed in Escherichia coli using autoinduction-based fermentation medium. There are numerous recombinant proteins functionally expressed in E. coli using this autoinduction approach. Preliminary media trials have shown that this medium is capable of producing a high cell density batch culture as claimed. Since the level of soluble expression is both protein and host/strain specific, further screening to identify significant media components affecting biomass production is therefore required. Currently, screening of full autoinduction medium components has yet to be examined elsewhere in the literature due to its tedious nature. Hence, the application of a fractional factorial design for identifying significant components in the autoinduction formulation was reported. Statistical analysis showed that glucose, glycerol and L-arabinose were most significant components influencing the biomass production. However, their effects were demoting rather than promoting the biomass production at elevated concentrations in shake flask culture. The highest biomass production (7.3 g/l) was achieved at low levels (-1). This represents 0.05 % (w/v) glucose, 0.5 % (w/v) glycerol, without the need of additional L-arabinose inducer. For a low number of experimental runs, this statistical approach has been proven efficient for screening vital medium components in comparison to conventional method. Journal of Pure and Applied Microbiology 2014-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38448/1/May_Jamil_JPAM.pdf application/pdf en http://irep.iium.edu.my/38448/3/38448_Screening%20of%20important_scopus.pdf Jamaluddin, Mohd Jamil Aizat and Amid, Azura and Azmi, Azlin Suhaida and Othman, Muhd. Ezza Faiez (2014) Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain. Journal of Pure and Applied Microbiology, 8 (SPEC. ISS. 1). pp. 741-750. ISSN 0973-7510 http://www.microbiologyjournal.org/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Jamaluddin, Mohd Jamil Aizat Amid, Azura Azmi, Azlin Suhaida Othman, Muhd. Ezza Faiez Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
description	Bromelain, a naturally available therapeutic protease enzyme from pineapple stem, was expressed in Escherichia coli using autoinduction-based fermentation medium. There are numerous recombinant proteins functionally expressed in E. coli using this autoinduction approach. Preliminary media trials have shown that this medium is capable of producing a high cell density batch culture as claimed. Since the level of soluble expression is both protein and host/strain specific, further screening to identify significant media components affecting biomass production is therefore required. Currently, screening of full autoinduction medium components has yet to be examined elsewhere in the literature due to its tedious nature. Hence, the application of a fractional factorial design for identifying significant components in the autoinduction formulation was reported. Statistical analysis showed that glucose, glycerol and L-arabinose were most significant components influencing the biomass production. However, their effects were demoting rather than promoting the biomass production at elevated concentrations in shake flask culture. The highest biomass production (7.3 g/l) was achieved at low levels (-1). This represents 0.05 % (w/v) glucose, 0.5 % (w/v) glycerol, without the need of additional L-arabinose inducer. For a low number of experimental runs, this statistical approach has been proven efficient for screening vital medium components in comparison to conventional method.
format	Article
author	Jamaluddin, Mohd Jamil Aizat Amid, Azura Azmi, Azlin Suhaida Othman, Muhd. Ezza Faiez
author_facet	Jamaluddin, Mohd Jamil Aizat Amid, Azura Azmi, Azlin Suhaida Othman, Muhd. Ezza Faiez
author_sort	Jamaluddin, Mohd Jamil Aizat
title	Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
title_short	Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
title_full	Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
title_fullStr	Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
title_full_unstemmed	Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
title_sort	screening of important autoinduction medium composition for high biaomass production of e. coli expressing recombinant bromelain
publisher	Journal of Pure and Applied Microbiology
publishDate	2014
url	http://irep.iium.edu.my/38448/ http://irep.iium.edu.my/38448/ http://irep.iium.edu.my/38448/1/May_Jamil_JPAM.pdf http://irep.iium.edu.my/38448/3/38448_Screening%20of%20important_scopus.pdf
first_indexed	2023-09-18T20:55:15Z
last_indexed	2023-09-18T20:55:15Z
_version_	1777410260496023552

Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain

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