The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model

In recent years, the use of bone marrow mesenchymal stem cell (BMSC) implantation has provided an alternative treatment for osteoarthritis. The objective of this study is to determine whether or not an intraarticular injection of a single dose of autologous chondrogenic induced BMSC could retard t...

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Main Authors:	Alfaqeh, Hamoud Hussein Mohammed, Mohamad Yahya, Nor Hamdan, Hui , Cheng Chen, Saim , Aminuddin, Idrus , Ruszymah
Format:	Article
Language:	English
Published:	ELSEVIER AND SciVerse ScienceDirect 2012
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/38291/ http://irep.iium.edu.my/38291/ http://irep.iium.edu.my/38291/ http://irep.iium.edu.my/38291/4/the_potential_of_intra-articular_injection.pdf

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spelling	iium-382912015-12-18T03:32:57Z http://irep.iium.edu.my/38291/ The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model Alfaqeh, Hamoud Hussein Mohammed Mohamad Yahya, Nor Hamdan Hui , Cheng Chen Saim , Aminuddin Idrus , Ruszymah R Medicine (General) In recent years, the use of bone marrow mesenchymal stem cell (BMSC) implantation has provided an alternative treatment for osteoarthritis. The objective of this study is to determine whether or not an intraarticular injection of a single dose of autologous chondrogenic induced BMSC could retard the progressive destruction of cartilage in a surgically induced osteoarthritis in sheep. Sheep BMSCs were isolated and divided into two groups. One group was cultured in chondrogenic media containing (Ham's F12:DMEM, 1:1) FD+1% FBS+5 ng/ml TGFβ3+50 ng/ml IGF-1 (CM), and the other group was cultured in the basal media, FD+10% FBS (BM). The procedure for surgically induced osteoarthritis was performed on the donor sheep 6 weeks prior to intra-articular injection into the knee joint of a single dose of BMSC from either group, suspended in 5 ml FD at density of 2 million cells/ml. The control groups were injected with basal media, without cells. Six weeks after injection, gross evidence of retardation of cartilage destruction was seen in the osteoarthritic knee joints treated with CM as well as BM. No significant ICRS (International Cartilage Repair Society) scoring was detected between the two groups with cells. However macroscopically, meniscus repair was observed in the knee joint treated with CM. Severe osteoarthritis and meniscal injury was observed in the control group. Interestingly, histologically the CM group demonstrated good cartilage histoarchitecture, thickness and quality, comparable to normal knee joint cartilage. As a conclusion, intra-articular injection of a single dose of BMSC either chondrogenically induced or not, could retard the progression of osteoarthritis (OA) in a sheep model, but the induced cells indicated better results especially in meniscus regeneration. ELSEVIER AND SciVerse ScienceDirect 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38291/4/the_potential_of_intra-articular_injection.pdf Alfaqeh, Hamoud Hussein Mohammed and Mohamad Yahya, Nor Hamdan and Hui , Cheng Chen and Saim , Aminuddin and Idrus , Ruszymah (2012) The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. Experimental Gerontology, 47 (6 ). pp. 458-464. ISSN ISSN: 0531-5565 http://www.elsevier.com/copyright 10.1016/j.exger.2012.03.018
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Alfaqeh, Hamoud Hussein Mohammed Mohamad Yahya, Nor Hamdan Hui , Cheng Chen Saim , Aminuddin Idrus , Ruszymah The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model
description	In recent years, the use of bone marrow mesenchymal stem cell (BMSC) implantation has provided an alternative treatment for osteoarthritis. The objective of this study is to determine whether or not an intraarticular injection of a single dose of autologous chondrogenic induced BMSC could retard the progressive destruction of cartilage in a surgically induced osteoarthritis in sheep. Sheep BMSCs were isolated and divided into two groups. One group was cultured in chondrogenic media containing (Ham's F12:DMEM, 1:1) FD+1% FBS+5 ng/ml TGFβ3+50 ng/ml IGF-1 (CM), and the other group was cultured in the basal media, FD+10% FBS (BM). The procedure for surgically induced osteoarthritis was performed on the donor sheep 6 weeks prior to intra-articular injection into the knee joint of a single dose of BMSC from either group, suspended in 5 ml FD at density of 2 million cells/ml. The control groups were injected with basal media, without cells. Six weeks after injection, gross evidence of retardation of cartilage destruction was seen in the osteoarthritic knee joints treated with CM as well as BM. No significant ICRS (International Cartilage Repair Society) scoring was detected between the two groups with cells. However macroscopically, meniscus repair was observed in the knee joint treated with CM. Severe osteoarthritis and meniscal injury was observed in the control group. Interestingly, histologically the CM group demonstrated good cartilage histoarchitecture, thickness and quality, comparable to normal knee joint cartilage. As a conclusion, intra-articular injection of a single dose of BMSC either chondrogenically induced or not, could retard the progression of osteoarthritis (OA) in a sheep model, but the induced cells indicated better results especially in meniscus regeneration.
format	Article
author	Alfaqeh, Hamoud Hussein Mohammed Mohamad Yahya, Nor Hamdan Hui , Cheng Chen Saim , Aminuddin Idrus , Ruszymah
author_facet	Alfaqeh, Hamoud Hussein Mohammed Mohamad Yahya, Nor Hamdan Hui , Cheng Chen Saim , Aminuddin Idrus , Ruszymah
author_sort	Alfaqeh, Hamoud Hussein Mohammed
title	The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model
title_short	The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model
title_full	The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model
title_fullStr	The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model
title_full_unstemmed	The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model
title_sort	potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model
publisher	ELSEVIER AND SciVerse ScienceDirect
publishDate	2012
url	http://irep.iium.edu.my/38291/ http://irep.iium.edu.my/38291/ http://irep.iium.edu.my/38291/ http://irep.iium.edu.my/38291/4/the_potential_of_intra-articular_injection.pdf
first_indexed	2023-09-18T20:55:00Z
last_indexed	2023-09-18T20:55:00Z
_version_	1777410244916281344

The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model

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