Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold

The aim of this research is to develop biocompatible nanofibrous mats using hydroxyethyl cellulose with improved cellular adhesion profiles and stability and use these fibrous mats as potential scaffold for skin tissue engineering. Glutaraldehyde was used to treat the scaffolds water insoluble as w...

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Main Authors:	Zulkifli, Farah Hanani, Hussain, Fathima Shahitha Jahir, Abdull Rasad, Mohammad Syaiful Bahari, Mohd Yusoff, Mashitah
Format:	Article
Language:	English
Published:	SAGE Publications 2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/38237/ http://irep.iium.edu.my/38237/ http://irep.iium.edu.my/38237/ http://irep.iium.edu.my/38237/1/Improved_cellular_response_of_chemically_crosslinked_collagen_incorporated_hydroxyethyl_cellulose_alchohol_nanofibers_scaffold_JBA.pdf

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spelling	iium-382372015-05-22T08:00:40Z http://irep.iium.edu.my/38237/ Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold Zulkifli, Farah Hanani Hussain, Fathima Shahitha Jahir Abdull Rasad, Mohammad Syaiful Bahari Mohd Yusoff, Mashitah Q Science (General) The aim of this research is to develop biocompatible nanofibrous mats using hydroxyethyl cellulose with improved cellular adhesion profiles and stability and use these fibrous mats as potential scaffold for skin tissue engineering. Glutaraldehyde was used to treat the scaffolds water insoluble as well as improve their biostability for possible use in biomedical applications. Electrospinning of hydroxyethyl cellulose (5 wt%) with poly(vinyl alcohol) (15 wt%) incorporated with and without collagen was blended at (1:1:1) and (1:1) ratios, respectively, and was evaluated for optimal criteria as tissue engineering scaffolds. The nanofibrous mats were crosslinked and characterized by scanning electron microscope, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetric analysis. Scanning electron microscope images showed that the mean diameters of blend nanofibers were gradually increased after chemically crosslinking with glutaraldehyde. Fourier transform infrared spectroscopy was carried out to understand chemical interactions in the presence of aldehyde groups. Thermal characterization results showed that the stability of hydroxyethyl cellulose/poly(vinyl alcohol) and hydroxyethyl cellulose/poly(vinyl alcohol)/collagen nanofibers was increased with glutaraldehyde treatment. Studies on cell–scaffolds interaction were carried out by culturing human fibroblast (hFOB) cells on the nanofibers by assessing the growth, proliferation, and morphologies of cells. The scanning electron microscope results show that better cell proliferation and attachment appeared on hydroxyethyl cellulose/poly(vinyl alcohol)/ collagen substrates after 7 days of culturing, thus, promoting the potential of electrospun scaffolds as a promising candidate for tissue engineering applications. SAGE Publications 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38237/1/Improved_cellular_response_of_chemically_crosslinked_collagen_incorporated_hydroxyethyl_cellulose_alchohol_nanofibers_scaffold_JBA.pdf Zulkifli, Farah Hanani and Hussain, Fathima Shahitha Jahir and Abdull Rasad, Mohammad Syaiful Bahari and Mohd Yusoff, Mashitah (2014) Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold. Journal of Biomaterials Applications. pp. 1-14. ISSN 0885-3282 (P) 1530-8022 (O) http://jba.sagepub.com/content/early/2014/09/02/0885328214549818 10.1177/0885328214549818
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Zulkifli, Farah Hanani Hussain, Fathima Shahitha Jahir Abdull Rasad, Mohammad Syaiful Bahari Mohd Yusoff, Mashitah Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold
description	The aim of this research is to develop biocompatible nanofibrous mats using hydroxyethyl cellulose with improved cellular adhesion profiles and stability and use these fibrous mats as potential scaffold for skin tissue engineering. Glutaraldehyde was used to treat the scaffolds water insoluble as well as improve their biostability for possible use in biomedical applications. Electrospinning of hydroxyethyl cellulose (5 wt%) with poly(vinyl alcohol) (15 wt%) incorporated with and without collagen was blended at (1:1:1) and (1:1) ratios, respectively, and was evaluated for optimal criteria as tissue engineering scaffolds. The nanofibrous mats were crosslinked and characterized by scanning electron microscope, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetric analysis. Scanning electron microscope images showed that the mean diameters of blend nanofibers were gradually increased after chemically crosslinking with glutaraldehyde. Fourier transform infrared spectroscopy was carried out to understand chemical interactions in the presence of aldehyde groups. Thermal characterization results showed that the stability of hydroxyethyl cellulose/poly(vinyl alcohol) and hydroxyethyl cellulose/poly(vinyl alcohol)/collagen nanofibers was increased with glutaraldehyde treatment. Studies on cell–scaffolds interaction were carried out by culturing human fibroblast (hFOB) cells on the nanofibers by assessing the growth, proliferation, and morphologies of cells. The scanning electron microscope results show that better cell proliferation and attachment appeared on hydroxyethyl cellulose/poly(vinyl alcohol)/ collagen substrates after 7 days of culturing, thus, promoting the potential of electrospun scaffolds as a promising candidate for tissue engineering applications.
format	Article
author	Zulkifli, Farah Hanani Hussain, Fathima Shahitha Jahir Abdull Rasad, Mohammad Syaiful Bahari Mohd Yusoff, Mashitah
author_facet	Zulkifli, Farah Hanani Hussain, Fathima Shahitha Jahir Abdull Rasad, Mohammad Syaiful Bahari Mohd Yusoff, Mashitah
author_sort	Zulkifli, Farah Hanani
title	Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold
title_short	Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold
title_full	Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold
title_fullStr	Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold
title_full_unstemmed	Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold
title_sort	improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold
publisher	SAGE Publications
publishDate	2014
url	http://irep.iium.edu.my/38237/ http://irep.iium.edu.my/38237/ http://irep.iium.edu.my/38237/ http://irep.iium.edu.my/38237/1/Improved_cellular_response_of_chemically_crosslinked_collagen_incorporated_hydroxyethyl_cellulose_alchohol_nanofibers_scaffold_JBA.pdf
first_indexed	2023-09-18T20:54:55Z
last_indexed	2023-09-18T20:54:55Z
_version_	1777410239823347712

Improved cellular response of chemically crosslinked collagen incorporated hydroxyethyl cellulose/poly(vinyl) alcohol nanofibers scaffold

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